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RP-HPLC analysis of methanol extract of Viscum articulatum - A plant from Ayurveda.

Abstract

BACKGROUND

Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. In ayurveda, the plant parts are used in "Kapha", "Vata", diseases of the blood, ulcer, and epilepsy. The plant parts are also used in urinary tract infection and wound infection. The plant contains five triterpenoids such as α-amyrin, lupeol, betulin, betulinic acid and oleanolic acid, exhibiting several pharmacological activities including antimicrobial, anti-HIV, antitumor, antiviral activity.

OBJECTIVE

To ensure the content of uniformity of oleanolic acid, a RP-HPLC method has been developed for estimation of oleanolic acid in V. articulatum aerial part.

MATERIAL AND METHODS

The RP-HPLC method was carried out in reverse phase C18 column, using methanol and water as mobile phase in the ratio of 95:5 (v/v), at the flow rate of 1 mL/min. The pH of aqueous phase was adjusted 3.2 with 1% (v/v) glacial acetic acid. The λmax was set at 210 nm.

RESULTS

The retention time of oleanolic acid was found at 21.5 ± 0.05 min. The linearity of the response was found to be 10-800 μg/mL. The coefficient of determinants of oleanolic acid was found to be (r2) 0.995 and equation Y = 19462X + 16,172. The LOD and LOQ were found to be for oleanolic acid (1.96% w/w) 0.197 ± 0.63 and 0.623 ± 0.87 μg/mL, respectively. The developed method was accurate, specific, precise and reproducible.

CONCLUSION

This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product.

Authors+Show Affiliations

School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India. Electronic address: naturalproductm@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30638917

Citation

Nag, Mrinmoy, et al. "RP-HPLC Analysis of Methanol Extract of Viscum Articulatum - a Plant From Ayurveda." Journal of Ayurveda and Integrative Medicine, 2019.
Nag M, Kar A, Chanda J, et al. RP-HPLC analysis of methanol extract of Viscum articulatum - A plant from Ayurveda. J Ayurveda Integr Med. 2019.
Nag, M., Kar, A., Chanda, J., & Mukherjee, P. K. (2019). RP-HPLC analysis of methanol extract of Viscum articulatum - A plant from Ayurveda. Journal of Ayurveda and Integrative Medicine, doi:10.1016/j.jaim.2018.02.135.
Nag M, et al. RP-HPLC Analysis of Methanol Extract of Viscum Articulatum - a Plant From Ayurveda. J Ayurveda Integr Med. 2019 Jan 10; PubMed PMID: 30638917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RP-HPLC analysis of methanol extract of Viscum articulatum - A plant from Ayurveda. AU - Nag,Mrinmoy, AU - Kar,Amit, AU - Chanda,Joydeb, AU - Mukherjee,Pulok K, Y1 - 2019/01/10/ PY - 2017/08/10/received PY - 2018/01/31/revised PY - 2018/02/21/accepted PY - 2019/1/15/entrez KW - Ayurveda KW - Method validation KW - Oleanolic acid KW - RP-HPLC KW - Viscum articulatum Burm JF - Journal of Ayurveda and integrative medicine JO - J Ayurveda Integr Med N2 - BACKGROUND: Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. In ayurveda, the plant parts are used in "Kapha", "Vata", diseases of the blood, ulcer, and epilepsy. The plant parts are also used in urinary tract infection and wound infection. The plant contains five triterpenoids such as α-amyrin, lupeol, betulin, betulinic acid and oleanolic acid, exhibiting several pharmacological activities including antimicrobial, anti-HIV, antitumor, antiviral activity. OBJECTIVE: To ensure the content of uniformity of oleanolic acid, a RP-HPLC method has been developed for estimation of oleanolic acid in V. articulatum aerial part. MATERIAL AND METHODS: The RP-HPLC method was carried out in reverse phase C18 column, using methanol and water as mobile phase in the ratio of 95:5 (v/v), at the flow rate of 1 mL/min. The pH of aqueous phase was adjusted 3.2 with 1% (v/v) glacial acetic acid. The λmax was set at 210 nm. RESULTS: The retention time of oleanolic acid was found at 21.5 ± 0.05 min. The linearity of the response was found to be 10-800 μg/mL. The coefficient of determinants of oleanolic acid was found to be (r2) 0.995 and equation Y = 19462X + 16,172. The LOD and LOQ were found to be for oleanolic acid (1.96% w/w) 0.197 ± 0.63 and 0.623 ± 0.87 μg/mL, respectively. The developed method was accurate, specific, precise and reproducible. CONCLUSION: This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product. SN - 0975-9476 UR - https://www.unboundmedicine.com/medline/citation/30638917/RP-HPLC_analysis_of_methanol_extract_of_Viscum_articulatum_-_A_plant_from_Ayurveda L2 - https://linkinghub.elsevier.com/retrieve/pii/S0975-9476(17)30476-X DB - PRIME DP - Unbound Medicine ER -