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Circulating Leptospira species identified in cattle of the Brazilian Amazon.
Acta Trop 2019; 191:212-216AT

Abstract

The present study aimed to detect the most prevalent serogroups and circulating Leptospira species in cows from Brazilian Amazon. Samples of blood serum, urine and kidney of 208 animals were collected at a municipal slaughterhouse in the Baixo Tocantins region of Pará State, Northern Brazil. The tests used were microscopic agglutination test (MAT), bacteriological isolation, polymerase chain reaction (PCR) and DNA sequencing. The frequency of MAT-reactive cows was 46.6% (97/208) with titers ranging from 100 to 3200, being Sejroe serogroup the most prevalent. There was no Leptospira isolation, but the DNA of bacterium was detected in 5.8% (12/208) of the kidney and in 14.9% (31/208) of the urine samples. DNA sequencing was performed directly from PCR products of 30 samples (3 kidneys and 27 urines), with identification of four different species: L. borgpetersenii with 56.7% (17/30), followed by L. kirschneri with 13.3% (4/30), L. interrogans with 6.7% (2/30), L. santarosai with 3.3% (1/30), and 20.0% (6/30) of samples were identified only at the genus level. These results reveal a diversity and peculiarity for bovine leptospirosis in the Amazon region, mainly due to the low frequency of L. santarosai and more surprising, the presence of L. kirschneri, differently of what is observed in other regions of Brazil.

Authors+Show Affiliations

Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.Instituto Federal de Educação Ciência e Tecnologia do Estado do Pará, Cametá, PA, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.Curso de Medicina Veterinária, Universidade Santo Amaro, UNISA, São Paulo, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: marcosbryan@usp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30639452

Citation

Guedes, Israel Barbosa, et al. "Circulating Leptospira Species Identified in Cattle of the Brazilian Amazon." Acta Tropica, vol. 191, 2019, pp. 212-216.
Guedes IB, Araújo SAA, de Souza GO, et al. Circulating Leptospira species identified in cattle of the Brazilian Amazon. Acta Trop. 2019;191:212-216.
Guedes, I. B., Araújo, S. A. A., de Souza, G. O., de Souza Silva, S. O., Taniwaki, S. A., Cortez, A., ... Heinemann, M. B. (2019). Circulating Leptospira species identified in cattle of the Brazilian Amazon. Acta Tropica, 191, pp. 212-216. doi:10.1016/j.actatropica.2019.01.011.
Guedes IB, et al. Circulating Leptospira Species Identified in Cattle of the Brazilian Amazon. Acta Trop. 2019;191:212-216. PubMed PMID: 30639452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating Leptospira species identified in cattle of the Brazilian Amazon. AU - Guedes,Israel Barbosa, AU - Araújo,Simone Aparecida de Almeida, AU - de Souza,Gisele Oliveira, AU - de Souza Silva,Sheila Oliveira, AU - Taniwaki,Sueli Akemi, AU - Cortez,Adriana, AU - Brandão,Paulo Eduardo, AU - Heinemann,Marcos Bryan, Y1 - 2019/01/11/ PY - 2018/11/13/received PY - 2019/01/09/revised PY - 2019/01/09/accepted PY - 2019/1/15/pubmed PY - 2019/4/10/medline PY - 2019/1/15/entrez KW - Amazonia KW - Bovine leptospirosis KW - DNA sequencing KW - Isolation KW - PCR SP - 212 EP - 216 JF - Acta tropica JO - Acta Trop. VL - 191 N2 - The present study aimed to detect the most prevalent serogroups and circulating Leptospira species in cows from Brazilian Amazon. Samples of blood serum, urine and kidney of 208 animals were collected at a municipal slaughterhouse in the Baixo Tocantins region of Pará State, Northern Brazil. The tests used were microscopic agglutination test (MAT), bacteriological isolation, polymerase chain reaction (PCR) and DNA sequencing. The frequency of MAT-reactive cows was 46.6% (97/208) with titers ranging from 100 to 3200, being Sejroe serogroup the most prevalent. There was no Leptospira isolation, but the DNA of bacterium was detected in 5.8% (12/208) of the kidney and in 14.9% (31/208) of the urine samples. DNA sequencing was performed directly from PCR products of 30 samples (3 kidneys and 27 urines), with identification of four different species: L. borgpetersenii with 56.7% (17/30), followed by L. kirschneri with 13.3% (4/30), L. interrogans with 6.7% (2/30), L. santarosai with 3.3% (1/30), and 20.0% (6/30) of samples were identified only at the genus level. These results reveal a diversity and peculiarity for bovine leptospirosis in the Amazon region, mainly due to the low frequency of L. santarosai and more surprising, the presence of L. kirschneri, differently of what is observed in other regions of Brazil. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/30639452/Circulating_Leptospira_species_identified_in_cattle_of_the_Brazilian_Amazon_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(18)31440-2 DB - PRIME DP - Unbound Medicine ER -