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The Effects of Diabetes and High-Fat Diet on Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium.
Invest Ophthalmol Vis Sci. 2019 01 02; 60(1):209-217.IO

Abstract

Purpose

There is a substantial body of evidence indicating that corneal sensory innervation is affected by pathology in a range of diseases. However, there are no published studies that have directly assessed whether the nerve fiber density of the different subpopulations of corneal sensory neurons are differentially affected. The present study explored the possibility that the intraepithelial nerve fiber density of corneal polymodal nociceptors and cold thermoreceptors are differentially affected in mice fed with a high-fat high cholesterol (HFHC; 21% fat, 2% cholesterol) diet and in those that also have diabetes.

Methods

The mice were fed the HFHC diet for the duration of the experiment (up to 40 weeks). Mice in the diabetes group had hyperglycaemia induced with streptozotocin after 15 weeks on the HFHC diet. Age-matched control animals were fed a standard diet. All corneal nerve fibers were labeled with a pan neuronal antibody (antiprotein gene product 9.5), and polymodal nociceptors and cold thermoreceptors were labeled with antibodies directed against transient receptor potential cation channel, subfamily V, member 1 and transient receptor potential cation channel subfamily M member 8, respectively.

Results

The mice fed a HFHC diet and those that in addition have hyperglycemia have similar reductions in corneal nerve fiber density consistent with small fiber neuropathy. Importantly, both treatments more markedly affected the intraepithelial axons of cold thermoreceptors than those of polymodal nociceptors.

Conclusions

The results provide evidence that distinct subpopulations of corneal sensory neurons can be differentially affected by pathology.

Authors+Show Affiliations

Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia.Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia.Department of Medicine, The University of Melbourne, Austin Health, Heidelberg, Victoria, Australia.Department of Medicine, The University of Melbourne, Austin Health, Heidelberg, Victoria, Australia.Department of Gastroenterology, Austin Health, Heidelberg, Victoria, Australia.Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30641549

Citation

Alamri, Abdulhakeem S., et al. "The Effects of Diabetes and High-Fat Diet On Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium." Investigative Ophthalmology & Visual Science, vol. 60, no. 1, 2019, pp. 209-217.
Alamri AS, Brock JA, Herath CB, et al. The Effects of Diabetes and High-Fat Diet on Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium. Invest Ophthalmol Vis Sci. 2019;60(1):209-217.
Alamri, A. S., Brock, J. A., Herath, C. B., Rajapaksha, I. G., Angus, P. W., & Ivanusic, J. J. (2019). The Effects of Diabetes and High-Fat Diet on Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium. Investigative Ophthalmology & Visual Science, 60(1), 209-217. https://doi.org/10.1167/iovs.18-25788
Alamri AS, et al. The Effects of Diabetes and High-Fat Diet On Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium. Invest Ophthalmol Vis Sci. 2019 01 2;60(1):209-217. PubMed PMID: 30641549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Effects of Diabetes and High-Fat Diet on Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium. AU - Alamri,Abdulhakeem S, AU - Brock,James A, AU - Herath,Chandana B, AU - Rajapaksha,Indu G, AU - Angus,Peter W, AU - Ivanusic,Jason J, PY - 2019/1/15/entrez PY - 2019/1/15/pubmed PY - 2019/6/25/medline SP - 209 EP - 217 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 60 IS - 1 N2 - Purpose: There is a substantial body of evidence indicating that corneal sensory innervation is affected by pathology in a range of diseases. However, there are no published studies that have directly assessed whether the nerve fiber density of the different subpopulations of corneal sensory neurons are differentially affected. The present study explored the possibility that the intraepithelial nerve fiber density of corneal polymodal nociceptors and cold thermoreceptors are differentially affected in mice fed with a high-fat high cholesterol (HFHC; 21% fat, 2% cholesterol) diet and in those that also have diabetes. Methods: The mice were fed the HFHC diet for the duration of the experiment (up to 40 weeks). Mice in the diabetes group had hyperglycaemia induced with streptozotocin after 15 weeks on the HFHC diet. Age-matched control animals were fed a standard diet. All corneal nerve fibers were labeled with a pan neuronal antibody (antiprotein gene product 9.5), and polymodal nociceptors and cold thermoreceptors were labeled with antibodies directed against transient receptor potential cation channel, subfamily V, member 1 and transient receptor potential cation channel subfamily M member 8, respectively. Results: The mice fed a HFHC diet and those that in addition have hyperglycemia have similar reductions in corneal nerve fiber density consistent with small fiber neuropathy. Importantly, both treatments more markedly affected the intraepithelial axons of cold thermoreceptors than those of polymodal nociceptors. Conclusions: The results provide evidence that distinct subpopulations of corneal sensory neurons can be differentially affected by pathology. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/30641549/The_Effects_of_Diabetes_and_High_Fat_Diet_on_Polymodal_Nociceptor_and_Cold_Thermoreceptor_Nerve_Terminal_Endings_in_the_Corneal_Epithelium_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.18-25788 DB - PRIME DP - Unbound Medicine ER -