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LoiA directly represses lon gene expression to activate the expression of Salmonella pathogenicity island-1 genes.
Res Microbiol. 2019 Apr - May; 170(3):131-137.RM

Abstract

Salmonella enterica serovar Typhimurium uses virulence factors encoded by Salmonella pathogenicity island 1 (SPI-1) to invade the intestinal epithelium. The low oxygen-induced transcriptional regulator LoiA is established to be a positive regulator of SPI-1 genes and can also repress the expression of the ATP-dependent Lon protease, a negative regulator of SPI-1 genes. Whether the repression of lon by LoiA is associated with its regulation of SPI-1 genes, as well as the regulatory mechanism involved, is unknown. In this study, we showed that LoiA directly represses the expression of the lon gene during invasion by binding to the promoter region of lon. The activation of S. Typhimurium SPI-1 gene expression by LoiA is associated with its repression of lon. Moreover, through invasion and mouse infection assays, we found that the repression of lon by LoiA contributes to S. Typhimurium invasion of intestinal epithelial cells and virulence in mice. However, LoiA does not influence lon expression during S. Typhimurium growth in macrophages, which may be associated with the low expression level of loiA in macrophages. Collectively, these findings establish the direct regulation of lon by LoiA and describe a novel mechanism by which LoiA regulates SPI-1 gene expression.

Authors+Show Affiliations

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China. Electronic address: jianglingyan@nankai.edu.cn.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China. Electronic address: lxmxiaomin0316@163.com.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China. Electronic address: lvrunxia@mail.nankai.edu.cn.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China. Electronic address: fenglu63@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30648612

Citation

Jiang, Lingyan, et al. "LoiA Directly Represses Lon Gene Expression to Activate the Expression of Salmonella Pathogenicity Island-1 Genes." Research in Microbiology, vol. 170, no. 3, 2019, pp. 131-137.
Jiang L, Li X, Lv R, et al. LoiA directly represses lon gene expression to activate the expression of Salmonella pathogenicity island-1 genes. Res Microbiol. 2019;170(3):131-137.
Jiang, L., Li, X., Lv, R., & Feng, L. (2019). LoiA directly represses lon gene expression to activate the expression of Salmonella pathogenicity island-1 genes. Research in Microbiology, 170(3), 131-137. https://doi.org/10.1016/j.resmic.2019.01.001
Jiang L, et al. LoiA Directly Represses Lon Gene Expression to Activate the Expression of Salmonella Pathogenicity Island-1 Genes. Res Microbiol. 2019 Apr - May;170(3):131-137. PubMed PMID: 30648612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LoiA directly represses lon gene expression to activate the expression of Salmonella pathogenicity island-1 genes. AU - Jiang,Lingyan, AU - Li,Xiaomin, AU - Lv,Runxia, AU - Feng,Lu, Y1 - 2019/01/12/ PY - 2018/10/09/received PY - 2019/01/02/revised PY - 2019/01/08/accepted PY - 2019/1/17/pubmed PY - 2019/8/28/medline PY - 2019/1/17/entrez KW - Invasion KW - Salmonella Typhimurium KW - Virulence SP - 131 EP - 137 JF - Research in microbiology JO - Res Microbiol VL - 170 IS - 3 N2 - Salmonella enterica serovar Typhimurium uses virulence factors encoded by Salmonella pathogenicity island 1 (SPI-1) to invade the intestinal epithelium. The low oxygen-induced transcriptional regulator LoiA is established to be a positive regulator of SPI-1 genes and can also repress the expression of the ATP-dependent Lon protease, a negative regulator of SPI-1 genes. Whether the repression of lon by LoiA is associated with its regulation of SPI-1 genes, as well as the regulatory mechanism involved, is unknown. In this study, we showed that LoiA directly represses the expression of the lon gene during invasion by binding to the promoter region of lon. The activation of S. Typhimurium SPI-1 gene expression by LoiA is associated with its repression of lon. Moreover, through invasion and mouse infection assays, we found that the repression of lon by LoiA contributes to S. Typhimurium invasion of intestinal epithelial cells and virulence in mice. However, LoiA does not influence lon expression during S. Typhimurium growth in macrophages, which may be associated with the low expression level of loiA in macrophages. Collectively, these findings establish the direct regulation of lon by LoiA and describe a novel mechanism by which LoiA regulates SPI-1 gene expression. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/30648612/LoiA_directly_represses_lon_gene_expression_to_activate_the_expression_of_Salmonella_pathogenicity_island_1_genes_ DB - PRIME DP - Unbound Medicine ER -