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Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus.
Int J Biol Macromol 2019; 127:406-414IJ

Abstract

Bromelain, a protease from pineapple plant can be applied as oral drug for the treatment of inflammation and certain diseases. Unlike most conventional supports, immobilization on edible support will make the enzyme suitable for therapeutic use. In this study, spores of probiotic Bacillus sp was used for the adsorption of bromelain. Effect of pH, temperature and enzyme concentration on bromelain immobilization was studied, followed by characterization of the enzymes. Maximum bromelain coupling (%) (50.607 ± 4.194) was obtained when immobilization was carried out at pH 6.0, 24 °C for 150 min. The immobilized enzyme showed optimum activity at pH 8 and 80 °C, while the free enzyme had 6 and 60 °C as its optimum pH and temperature, respectively. Bromelain Vmax increased after immobilization while Km decreased. Activation energy, Ea was 26.513 kJ/mol and 20.942 kJ/mol for the free and immobilized enzymes, respectively. The immobilized bromelain also showed significantly greater storage and thermal stability than the free bromelain. At 80 °C, the free bromelain lost all its activity after 50 min while the immobilized enzyme lost only 46.89% activity. Bromelain was successfully immobilized on Bacillus spores with improved catalytic and non-catalytic properties and this holds great potential for its growing therapeutic applications.

Authors+Show Affiliations

Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Electronic address: tochukwu.nwagu@unn.edu.ng.Department of Microbiology, Faculty of Biological Sciences, University of Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30654039

Citation

Nwagu, Tochukwu Nwamaka, and Chika Jude Ugwuodo. "Stabilizing Bromelain for Therapeutic Applications By Adsorption Immobilization On Spores of Probiotic Bacillus." International Journal of Biological Macromolecules, vol. 127, 2019, pp. 406-414.
Nwagu TN, Ugwuodo CJ. Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus. Int J Biol Macromol. 2019;127:406-414.
Nwagu, T. N., & Ugwuodo, C. J. (2019). Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus. International Journal of Biological Macromolecules, 127, pp. 406-414. doi:10.1016/j.ijbiomac.2019.01.061.
Nwagu TN, Ugwuodo CJ. Stabilizing Bromelain for Therapeutic Applications By Adsorption Immobilization On Spores of Probiotic Bacillus. Int J Biol Macromol. 2019 Apr 15;127:406-414. PubMed PMID: 30654039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus. AU - Nwagu,Tochukwu Nwamaka, AU - Ugwuodo,Chika Jude, Y1 - 2019/01/15/ PY - 2018/07/24/received PY - 2019/01/03/revised PY - 2019/01/14/accepted PY - 2019/1/18/pubmed PY - 2019/6/21/medline PY - 2019/1/18/entrez SP - 406 EP - 414 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 127 N2 - Bromelain, a protease from pineapple plant can be applied as oral drug for the treatment of inflammation and certain diseases. Unlike most conventional supports, immobilization on edible support will make the enzyme suitable for therapeutic use. In this study, spores of probiotic Bacillus sp was used for the adsorption of bromelain. Effect of pH, temperature and enzyme concentration on bromelain immobilization was studied, followed by characterization of the enzymes. Maximum bromelain coupling (%) (50.607 ± 4.194) was obtained when immobilization was carried out at pH 6.0, 24 °C for 150 min. The immobilized enzyme showed optimum activity at pH 8 and 80 °C, while the free enzyme had 6 and 60 °C as its optimum pH and temperature, respectively. Bromelain Vmax increased after immobilization while Km decreased. Activation energy, Ea was 26.513 kJ/mol and 20.942 kJ/mol for the free and immobilized enzymes, respectively. The immobilized bromelain also showed significantly greater storage and thermal stability than the free bromelain. At 80 °C, the free bromelain lost all its activity after 50 min while the immobilized enzyme lost only 46.89% activity. Bromelain was successfully immobilized on Bacillus spores with improved catalytic and non-catalytic properties and this holds great potential for its growing therapeutic applications. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30654039/Stabilizing_bromelain_for_therapeutic_applications_by_adsorption_immobilization_on_spores_of_probiotic_Bacillus L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)33833-9 DB - PRIME DP - Unbound Medicine ER -