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Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots.
World J Microbiol Biotechnol. 2019 Jan 17; 35(2):20.WJ

Abstract

Endophytes have been reported from all plant species from different parts of tissue including root, stem and leaves. Here we report, three fungal endophytes, Aspergillus terreus strain 2aWF (2aWF), Penicillium oxalicum strain 5aWF (5aWF), and Sarocladium kiliense strain 10aWF (10aWF) from Withania somnifera, which could enhance withanolides content in leaf and root. Upon treatment with the above endophytes to 4 weeks old plants in field conditions, W. somnifera elicited withanolide A content (97 to 100%) in leaves without considerable changes in withaferin A content. Furthermore, withanolide A content in roots of 5aWF and 10aWF endophyte treated W. somnifera plants increased up to 52% and 65% respectively. Incidentally, expression profile of withanolide and sterol biosynthetic pathway genes HMGR, DXR, FPPS, SQS, SQE, CAS, SMT1, STE1 and CYP710A1 were significantly upregulated in 2aWF, 5aWF and 10aWF fungal endophyte treated plants. Besides, modulation of withanolide biosynthetic pathway genes, fungal endophytes also induce a host resistant related gene, NPR1 resulting in 2, 4 and 16 fold expression levels in 2aWF, 10aWF and 5aWF endophyte treatments respectively, compared to control plants. Overall, our results illustrate that application of native-fungal endophytes 2aWF (96.60%), 5aWF (95%) and 10aWF (147%) enhances plant biomass in addition to withanolide content.

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Authors+Show Affiliations

Kushwaha RK
Microbial Technology Laboratory, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore, 56065, India. Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India.
Singh S
Microbial Technology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India. Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India.
Pandey SS
Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India.
Kalra A
Microbial Technology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India. Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India.
Babu CSV
Microbial Technology Laboratory, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore, 56065, India. vivekbabu.cs@cimap.res.in. Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India. vivekbabu.cs@cimap.res.in.

MeSH

AspergillusBiosynthetic PathwaysEndophytesGene Expression Regulation, PlantGene Regulatory NetworksHypocrealesPenicilliumPlant LeavesPlant ProteinsPlant RootsPlant StemsWithaniaWithanolides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30656434

Citation

Kushwaha, Ramesh Kumar, et al. "Fungal Endophytes Attune Withanolide Biosynthesis in Withania Somnifera, Prime to Enhanced Withanolide a Content in Leaves and Roots." World Journal of Microbiology & Biotechnology, vol. 35, no. 2, 2019, p. 20.
Kushwaha RK, Singh S, Pandey SS, et al. Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots. World J Microbiol Biotechnol. 2019;35(2):20.
Kushwaha, R. K., Singh, S., Pandey, S. S., Kalra, A., & Babu, C. S. V. (2019). Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots. World Journal of Microbiology & Biotechnology, 35(2), 20. https://doi.org/10.1007/s11274-019-2593-1
Kushwaha RK, et al. Fungal Endophytes Attune Withanolide Biosynthesis in Withania Somnifera, Prime to Enhanced Withanolide a Content in Leaves and Roots. World J Microbiol Biotechnol. 2019 Jan 17;35(2):20. PubMed PMID: 30656434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots. AU - Kushwaha,Ramesh Kumar, AU - Singh,Sucheta, AU - Pandey,Shiv Shanker, AU - Kalra,Alok, AU - Babu,C S Vivek, Y1 - 2019/01/17/ PY - 2018/09/19/received PY - 2019/01/11/accepted PY - 2019/1/19/entrez PY - 2019/1/19/pubmed PY - 2019/1/27/medline KW - Aspergillus terreus KW - Endophytes KW - Penicillium oxalicum KW - Sarocladium kiliense KW - Withaferin A and Withanolide A KW - Withania somnifera SP - 20 EP - 20 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 35 IS - 2 N2 - Endophytes have been reported from all plant species from different parts of tissue including root, stem and leaves. Here we report, three fungal endophytes, Aspergillus terreus strain 2aWF (2aWF), Penicillium oxalicum strain 5aWF (5aWF), and Sarocladium kiliense strain 10aWF (10aWF) from Withania somnifera, which could enhance withanolides content in leaf and root. Upon treatment with the above endophytes to 4 weeks old plants in field conditions, W. somnifera elicited withanolide A content (97 to 100%) in leaves without considerable changes in withaferin A content. Furthermore, withanolide A content in roots of 5aWF and 10aWF endophyte treated W. somnifera plants increased up to 52% and 65% respectively. Incidentally, expression profile of withanolide and sterol biosynthetic pathway genes HMGR, DXR, FPPS, SQS, SQE, CAS, SMT1, STE1 and CYP710A1 were significantly upregulated in 2aWF, 5aWF and 10aWF fungal endophyte treated plants. Besides, modulation of withanolide biosynthetic pathway genes, fungal endophytes also induce a host resistant related gene, NPR1 resulting in 2, 4 and 16 fold expression levels in 2aWF, 10aWF and 5aWF endophyte treatments respectively, compared to control plants. Overall, our results illustrate that application of native-fungal endophytes 2aWF (96.60%), 5aWF (95%) and 10aWF (147%) enhances plant biomass in addition to withanolide content. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/30656434/Fungal_endophytes_attune_withanolide_biosynthesis_in_Withania_somnifera_prime_to_enhanced_withanolide_A_content_in_leaves_and_roots_ DB - PRIME DP - Unbound Medicine ER -