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First look into the venom of Roatan Island's critically endangered coral snake Micrurus ruatanus: Proteomic characterization, toxicity, immunorecognition and neutralization by an antivenom.
J Proteomics. 2019 04 30; 198:177-185.JP

Abstract

A proteomic and toxicological study of the venom from one specimen of Micrurus ruatanus, a critically endangered coral snake species endemic to Roatan Island, Honduras, was carried out. Immunorecognition and neutralization of venom lethality by an anticoral antivenom was also evaluated. Forty peaks were collected from RP-HPLC fractionation of the venom. After SDS-PAGE analysis, fifty-eight bands were examined by MALDI-TOF/TOF mass spectrometry. Micrurus ruatanus venom displayed a three-finger toxin (3FTx)-rich venom phenotype, as well as a significant amount of phospholipases A2 (PLA2s). Various other proteins were identified, including Kunitz-type inhibitor proteins, L-amino acid oxidases, C-type lectin/lectin-like, metalloproteinases, serine proteinases, vespryn/ohanin, 5'-nucleotidases, glutathione peroxidases, and phosphodiesterases. Micrurus ruatanus venom displayed significant PLA2 activity in vitro and myotoxicity in vivo. The venom showed high lethal potency in mice, being one of the most lethal in Central America. The anticoral antivenom (SAC-ICP) produced by Instituto Clodomiro Picado neutralized the lethal activity of the venom. Major fractions with relevant lethal activity were also identified by a screening analysis.

SIGNIFICANCE:

The proteomic characterization, toxicity, immunorecognition and neutralization of Micrurus ruatanus venom have been determined for the first time. This coral snake is endemic to Roatan Island and contains a three-finger toxin-rich venom that displayed a potent lethal activity in mice. The anticoral antivenom produced by Instituto Clodomiro Picado neutralized the lethal activity of this venom in vivo, and therefore should be effective in the treatment of envenomings by this snake.

Authors+Show Affiliations

Department of Human Genetics, University of Debrecen, Debrecen, Hungary.Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Hungary.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: julian.fernandezulate@ucr.ac.cr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30659935

Citation

Lippa, Enikő, et al. "First Look Into the Venom of Roatan Island's Critically Endangered Coral Snake Micrurus Ruatanus: Proteomic Characterization, Toxicity, Immunorecognition and Neutralization By an Antivenom." Journal of Proteomics, vol. 198, 2019, pp. 177-185.
Lippa E, Török F, Gómez A, et al. First look into the venom of Roatan Island's critically endangered coral snake Micrurus ruatanus: Proteomic characterization, toxicity, immunorecognition and neutralization by an antivenom. J Proteomics. 2019;198:177-185.
Lippa, E., Török, F., Gómez, A., Corrales, G., Chacón, D., Sasa, M., Gutiérrez, J. M., Lomonte, B., & Fernández, J. (2019). First look into the venom of Roatan Island's critically endangered coral snake Micrurus ruatanus: Proteomic characterization, toxicity, immunorecognition and neutralization by an antivenom. Journal of Proteomics, 198, 177-185. https://doi.org/10.1016/j.jprot.2019.01.007
Lippa E, et al. First Look Into the Venom of Roatan Island's Critically Endangered Coral Snake Micrurus Ruatanus: Proteomic Characterization, Toxicity, Immunorecognition and Neutralization By an Antivenom. J Proteomics. 2019 04 30;198:177-185. PubMed PMID: 30659935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First look into the venom of Roatan Island's critically endangered coral snake Micrurus ruatanus: Proteomic characterization, toxicity, immunorecognition and neutralization by an antivenom. AU - Lippa,Enikő, AU - Török,Ferenc, AU - Gómez,Aarón, AU - Corrales,Greivin, AU - Chacón,Danilo, AU - Sasa,Mahmood, AU - Gutiérrez,José María, AU - Lomonte,Bruno, AU - Fernández,Julián, Y1 - 2019/01/17/ PY - 2018/12/16/received PY - 2019/01/11/revised PY - 2019/01/14/accepted PY - 2019/1/20/pubmed PY - 2020/7/9/medline PY - 2019/1/20/entrez KW - Coral snake KW - Micrurus ruatanus KW - Venomics SP - 177 EP - 185 JF - Journal of proteomics JO - J Proteomics VL - 198 N2 - A proteomic and toxicological study of the venom from one specimen of Micrurus ruatanus, a critically endangered coral snake species endemic to Roatan Island, Honduras, was carried out. Immunorecognition and neutralization of venom lethality by an anticoral antivenom was also evaluated. Forty peaks were collected from RP-HPLC fractionation of the venom. After SDS-PAGE analysis, fifty-eight bands were examined by MALDI-TOF/TOF mass spectrometry. Micrurus ruatanus venom displayed a three-finger toxin (3FTx)-rich venom phenotype, as well as a significant amount of phospholipases A2 (PLA2s). Various other proteins were identified, including Kunitz-type inhibitor proteins, L-amino acid oxidases, C-type lectin/lectin-like, metalloproteinases, serine proteinases, vespryn/ohanin, 5'-nucleotidases, glutathione peroxidases, and phosphodiesterases. Micrurus ruatanus venom displayed significant PLA2 activity in vitro and myotoxicity in vivo. The venom showed high lethal potency in mice, being one of the most lethal in Central America. The anticoral antivenom (SAC-ICP) produced by Instituto Clodomiro Picado neutralized the lethal activity of the venom. Major fractions with relevant lethal activity were also identified by a screening analysis. SIGNIFICANCE: The proteomic characterization, toxicity, immunorecognition and neutralization of Micrurus ruatanus venom have been determined for the first time. This coral snake is endemic to Roatan Island and contains a three-finger toxin-rich venom that displayed a potent lethal activity in mice. The anticoral antivenom produced by Instituto Clodomiro Picado neutralized the lethal activity of this venom in vivo, and therefore should be effective in the treatment of envenomings by this snake. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/30659935/First_look_into_the_venom_of_Roatan_Island's_critically_endangered_coral_snake_Micrurus_ruatanus:_Proteomic_characterization_toxicity_immunorecognition_and_neutralization_by_an_antivenom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(19)30015-6 DB - PRIME DP - Unbound Medicine ER -