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Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency.
Biol Trace Elem Res. 2019 Oct; 191(2):276-285.BT

Abstract

The objective of this study is to investigate changes in the expression of enzymes involved in chondroitin sulfate (CS) sulfation in distal articular surface of proximal interphalangeal joint isolated from school-age children patients with Kashin-Beck disease (KBD), using normal children as controls. Articular cartilage samples were collected from four normal and four KBD children (7-12 years old), and these children were assigned to control and KBD groups. Hematoxylin and eosin (H&E), toluidine blue (TB), and immunohistochemical (IHC) stainings were utilized to evaluate changes in joint pathology and expression of enzymes involved in CS sulfation, including carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), and uronyl 2-O-sulfotransferase (UST). The correspondence results were examined by semi-quantitative analysis. Compared with the control group, the KBD group showed the following: a significant decrease of total chondrocytes in superficial, middle, and deep layers and deposition of sulfated glycosaminoglycans in extracellular matrix of KBD cartilage were observed; positive staining chondrocytes of CHST-12, CHST-13, and UST were significantly less in superficial zone of KBD cartilage; and CHST-13 positive staining chondrocytes was reduced in deep zone of KBD cartilage. In contrast, the positive staining rates of CHST-12, CHST-13, and UST in KBD were significantly higher than those in the control group. The decreased expression of these enzymes and the physiologic compensatory reaction may be the signs of early-stage KBD. The alterations of CS structure modifying sulfotransferases in finger articular cartilage might play an important role in the onset and pathogenesis of school-age KBD children.

Authors+Show Affiliations

Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Department of Ophthalmology, the First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Department of Integrative Medical Biology, Umeå University, 90187, Umeå, Sweden.Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. College of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.Key laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. bbbishop@126.com. College of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China. bbbishop@126.com. Shenzhen Institute, Xi'an Jiaotong University, Shenzhen, 518057, Guangzhou, People's Republic of China. bbbishop@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30661165

Citation

Guo, Yijie, et al. "Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children With Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused By Selenium Deficiency." Biological Trace Element Research, vol. 191, no. 2, 2019, pp. 276-285.
Guo Y, Zhou Y, Yan S, et al. Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency. Biol Trace Elem Res. 2019;191(2):276-285.
Guo, Y., Zhou, Y., Yan, S., Qu, C., Wang, L., Guo, X., & Han, J. (2019). Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency. Biological Trace Element Research, 191(2), 276-285. https://doi.org/10.1007/s12011-019-1642-9
Guo Y, et al. Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children With Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused By Selenium Deficiency. Biol Trace Elem Res. 2019;191(2):276-285. PubMed PMID: 30661165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency. AU - Guo,Yijie, AU - Zhou,Yuan, AU - Yan,Siqi, AU - Qu,Chengjuan, AU - Wang,Liyun, AU - Guo,Xiong, AU - Han,Jing, Y1 - 2019/01/19/ PY - 2018/10/12/received PY - 2019/01/14/accepted PY - 2019/1/21/pubmed PY - 2020/1/21/medline PY - 2019/1/21/entrez KW - Child cartilage KW - Chondroitin sulfate KW - Kashin–Beck disease KW - Selenium deficiency KW - Sulfation KW - Sulfotransferases SP - 276 EP - 285 JF - Biological trace element research JO - Biol Trace Elem Res VL - 191 IS - 2 N2 - The objective of this study is to investigate changes in the expression of enzymes involved in chondroitin sulfate (CS) sulfation in distal articular surface of proximal interphalangeal joint isolated from school-age children patients with Kashin-Beck disease (KBD), using normal children as controls. Articular cartilage samples were collected from four normal and four KBD children (7-12 years old), and these children were assigned to control and KBD groups. Hematoxylin and eosin (H&E), toluidine blue (TB), and immunohistochemical (IHC) stainings were utilized to evaluate changes in joint pathology and expression of enzymes involved in CS sulfation, including carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), and uronyl 2-O-sulfotransferase (UST). The correspondence results were examined by semi-quantitative analysis. Compared with the control group, the KBD group showed the following: a significant decrease of total chondrocytes in superficial, middle, and deep layers and deposition of sulfated glycosaminoglycans in extracellular matrix of KBD cartilage were observed; positive staining chondrocytes of CHST-12, CHST-13, and UST were significantly less in superficial zone of KBD cartilage; and CHST-13 positive staining chondrocytes was reduced in deep zone of KBD cartilage. In contrast, the positive staining rates of CHST-12, CHST-13, and UST in KBD were significantly higher than those in the control group. The decreased expression of these enzymes and the physiologic compensatory reaction may be the signs of early-stage KBD. The alterations of CS structure modifying sulfotransferases in finger articular cartilage might play an important role in the onset and pathogenesis of school-age KBD children. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/30661165/Decreased_Expression_of_CHST_12_CHST_13_and_UST_in_the_Proximal_Interphalangeal_Joint_Cartilage_of_School_Age_Children_with_Kashin_Beck_Disease:_an_Endemic_Osteoarthritis_in_China_Caused_by_Selenium_Deficiency_ L2 - https://dx.doi.org/10.1007/s12011-019-1642-9 DB - PRIME DP - Unbound Medicine ER -