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Protective Effect of Baicalein on Oxidative Stress-induced DNA Damage and Apoptosis in RT4-D6P2T Schwann Cells.
Int J Med Sci. 2019; 16(1):8-16.IJ

Abstract

Background:

Due to its high antioxidant activity, baicalein, a kind of flavonoid present in Radical Scutellariae, has various pharmacological effects. However, the protective effect against oxidative stress in Schwann cells, which plays an important role in peripheral neuropathy, has not yet been studied. In this study, the effects of baicalein on hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in RT4-D6P2T Schwann cells were evaluated.

Methods:

Cell viability assay was performed using MTT assay and colony formation assay. Apoptosis was assessed by flow cytometry analysis and DNA fragmentation assay. The effects on DNA damage and ATP content were analyzed by comet method and luminometer. In addition, changes in protein expression were observed by Western blotting.

Results:

Our results show that baicalein significantly inhibits H2O2-induced cytotoxicity through blocking reactive oxygen species (ROS) generation. We also demonstrate that baicalein is to block H2O2-induced DNA damage as evidenced by inhibition of DNA tail formation and γH2AX phosphorylation. Moreover, baicalein significantly attenuated H2O2-induced apoptosis and mitochondrial dysfunction, and restored inhibition of ATP production. The suppression of apoptosis by baicalein in H2O2-stimulated cells was associated with reduction of increased Bax/Bcl-2 ratio, activation of caspase-9 and -3, and degradation of poly (ADP-ribose) polymerase.

Conclusions:

These results demonstrate that baicalein eliminates H2O2-induced apoptosis through conservation of mitochondrial function by the removal of ROS. Therefore, it is suggested that baicalein protects Schwann cells from oxidative stress, and may be beneficial for the prevention and treatment of peripheral neuropathy induced by oxidative stress.

Authors+Show Affiliations

Department of Molecular Biology, College of Natural Sciences, Dong-eui University, Busan 47340, Republic of Korea.Anti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea. Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, Republic of Korea.Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.Department of Food and Nutrition, College of Nursing, Healthcare Sciences & Human Ecology, Dong-eui University, Busan 47340, Republic of Korea.Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University, Busan 47340, Republic of Korea.Department of Anatomy and Cell Biology, Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, Busan 49201, Republic of Korea.Department of Physiology, Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, Busan 49201, Republic of Korea.Anti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea. Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30662323

Citation

Park, Cheol, et al. "Protective Effect of Baicalein On Oxidative Stress-induced DNA Damage and Apoptosis in RT4-D6P2T Schwann Cells." International Journal of Medical Sciences, vol. 16, no. 1, 2019, pp. 8-16.
Park C, Choi EO, Kim GY, et al. Protective Effect of Baicalein on Oxidative Stress-induced DNA Damage and Apoptosis in RT4-D6P2T Schwann Cells. Int J Med Sci. 2019;16(1):8-16.
Park, C., Choi, E. O., Kim, G. Y., Hwang, H. J., Kim, B. W., Yoo, Y. H., Park, H. T., & Choi, Y. H. (2019). Protective Effect of Baicalein on Oxidative Stress-induced DNA Damage and Apoptosis in RT4-D6P2T Schwann Cells. International Journal of Medical Sciences, 16(1), 8-16. https://doi.org/10.7150/ijms.29692
Park C, et al. Protective Effect of Baicalein On Oxidative Stress-induced DNA Damage and Apoptosis in RT4-D6P2T Schwann Cells. Int J Med Sci. 2019;16(1):8-16. PubMed PMID: 30662323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Baicalein on Oxidative Stress-induced DNA Damage and Apoptosis in RT4-D6P2T Schwann Cells. AU - Park,Cheol, AU - Choi,Eun Ok, AU - Kim,Gi-Young, AU - Hwang,Hye-Jin, AU - Kim,Byung Woo, AU - Yoo,Young Hyun, AU - Park,Hwan Tae, AU - Choi,Yung Hyun, Y1 - 2019/01/01/ PY - 2018/09/04/received PY - 2018/10/31/accepted PY - 2019/1/22/entrez PY - 2019/1/22/pubmed PY - 2019/4/26/medline KW - Baicalein KW - DNA damage KW - Schwann cells KW - apoptosis KW - oxidative stress SP - 8 EP - 16 JF - International journal of medical sciences JO - Int J Med Sci VL - 16 IS - 1 N2 - Background: Due to its high antioxidant activity, baicalein, a kind of flavonoid present in Radical Scutellariae, has various pharmacological effects. However, the protective effect against oxidative stress in Schwann cells, which plays an important role in peripheral neuropathy, has not yet been studied. In this study, the effects of baicalein on hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in RT4-D6P2T Schwann cells were evaluated. Methods: Cell viability assay was performed using MTT assay and colony formation assay. Apoptosis was assessed by flow cytometry analysis and DNA fragmentation assay. The effects on DNA damage and ATP content were analyzed by comet method and luminometer. In addition, changes in protein expression were observed by Western blotting. Results: Our results show that baicalein significantly inhibits H2O2-induced cytotoxicity through blocking reactive oxygen species (ROS) generation. We also demonstrate that baicalein is to block H2O2-induced DNA damage as evidenced by inhibition of DNA tail formation and γH2AX phosphorylation. Moreover, baicalein significantly attenuated H2O2-induced apoptosis and mitochondrial dysfunction, and restored inhibition of ATP production. The suppression of apoptosis by baicalein in H2O2-stimulated cells was associated with reduction of increased Bax/Bcl-2 ratio, activation of caspase-9 and -3, and degradation of poly (ADP-ribose) polymerase. Conclusions: These results demonstrate that baicalein eliminates H2O2-induced apoptosis through conservation of mitochondrial function by the removal of ROS. Therefore, it is suggested that baicalein protects Schwann cells from oxidative stress, and may be beneficial for the prevention and treatment of peripheral neuropathy induced by oxidative stress. SN - 1449-1907 UR - https://www.unboundmedicine.com/medline/citation/30662323/Protective_Effect_of_Baicalein_on_Oxidative_Stress_induced_DNA_Damage_and_Apoptosis_in_RT4_D6P2T_Schwann_Cells_ L2 - https://www.medsci.org/v16p0008.htm DB - PRIME DP - Unbound Medicine ER -