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A Novel Model for Studying Voltage-Gated Ion Channel Gene Expression during Reversible Ischemic Stroke.
Int J Med Sci. 2019; 16(1):60-67.IJ

Abstract

The dysfunction of voltage-gated ion channels contributes to the pathology of ischemic stroke. In this study, we developed rat models of transient ischemic attack (TIA) and reversible ischemic neurological deficit (RIND) that was induced via the injection of artificial embolic particles during full consciousness, that allow us to monitor the neurologic deficit and positron emission tomography (PET) scans in real-time. We then evaluated the infarction volume of brain tissue was confirmed by 2,3,5-triphenyl tetrazolium chloride (TTC) staining, and gene expressions were evaluated by quantitative real-time PCR (qPCR). We found that rats with TIA or RIND exhibited neurological deficits as determined by negative TTC and PET findings. However, the expression of voltage-gated sodium channels in the hippocampus was significantly up-regulated in the qPCR array study. Furthermore, an altered expression of sodium channel β-subunits and potassium channels, were observed in RIND compared to TIA groups. In conclusion, to our knowledge, this is the first report of the successful evaluation of voltage-gated ion channel gene expression in TIA and RIND animal models. This model will aid future studies in investigating pathophysiological mechanisms, and in developing new therapeutic compounds for the treatment of TIA and RIND.

Authors+Show Affiliations

Department of Surgery, E-Da Hospital, Kaohsiung, Taiwan.Department of Orthopedic Surgery, E-Da Hospital, Kaohsiung, Taiwan.Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, MD, USA.School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan. Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan.School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.Department of Neurology, China Medical University Hospital, Taichung, Taiwan. School of Medicine, China Medical University, Taichung, Taiwan. Department of Neurology, China Medical University, An-Nan Hospital, Tainan, Taiwan.School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan. Department of Medical Research, E-Da Hospital/ E-Da Cancer Hospital, Kaohsiung, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30662329

Citation

Tai, Yun-Shen, et al. "A Novel Model for Studying Voltage-Gated Ion Channel Gene Expression During Reversible Ischemic Stroke." International Journal of Medical Sciences, vol. 16, no. 1, 2019, pp. 60-67.
Tai YS, Yang SC, Hsieh YC, et al. A Novel Model for Studying Voltage-Gated Ion Channel Gene Expression during Reversible Ischemic Stroke. Int J Med Sci. 2019;16(1):60-67.
Tai, Y. S., Yang, S. C., Hsieh, Y. C., Huang, Y. B., Wu, P. C., Tsai, M. J., Tsai, Y. H., & Lin, M. W. (2019). A Novel Model for Studying Voltage-Gated Ion Channel Gene Expression during Reversible Ischemic Stroke. International Journal of Medical Sciences, 16(1), 60-67. https://doi.org/10.7150/ijms.27442
Tai YS, et al. A Novel Model for Studying Voltage-Gated Ion Channel Gene Expression During Reversible Ischemic Stroke. Int J Med Sci. 2019;16(1):60-67. PubMed PMID: 30662329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Novel Model for Studying Voltage-Gated Ion Channel Gene Expression during Reversible Ischemic Stroke. AU - Tai,Yun-Shen, AU - Yang,Shih-Chieh, AU - Hsieh,Yi-Chun, AU - Huang,Yaw-Bin, AU - Wu,Pao-Chu, AU - Tsai,Ming-Jun, AU - Tsai,Yi-Hung, AU - Lin,Ming-Wei, Y1 - 2019/01/01/ PY - 2018/05/23/received PY - 2018/10/31/accepted PY - 2019/1/22/entrez PY - 2019/1/22/pubmed PY - 2019/4/26/medline KW - animal model KW - embolic stroke KW - reversible ischemic neurological deficit KW - transient ischemic attack KW - voltage-gated ion channels SP - 60 EP - 67 JF - International journal of medical sciences JO - Int J Med Sci VL - 16 IS - 1 N2 - The dysfunction of voltage-gated ion channels contributes to the pathology of ischemic stroke. In this study, we developed rat models of transient ischemic attack (TIA) and reversible ischemic neurological deficit (RIND) that was induced via the injection of artificial embolic particles during full consciousness, that allow us to monitor the neurologic deficit and positron emission tomography (PET) scans in real-time. We then evaluated the infarction volume of brain tissue was confirmed by 2,3,5-triphenyl tetrazolium chloride (TTC) staining, and gene expressions were evaluated by quantitative real-time PCR (qPCR). We found that rats with TIA or RIND exhibited neurological deficits as determined by negative TTC and PET findings. However, the expression of voltage-gated sodium channels in the hippocampus was significantly up-regulated in the qPCR array study. Furthermore, an altered expression of sodium channel β-subunits and potassium channels, were observed in RIND compared to TIA groups. In conclusion, to our knowledge, this is the first report of the successful evaluation of voltage-gated ion channel gene expression in TIA and RIND animal models. This model will aid future studies in investigating pathophysiological mechanisms, and in developing new therapeutic compounds for the treatment of TIA and RIND. SN - 1449-1907 UR - https://www.unboundmedicine.com/medline/citation/30662329/A_Novel_Model_for_Studying_Voltage-Gated_Ion_Channel_Gene_Expression_during_Reversible_Ischemic_Stroke. L2 - https://www.medsci.org/v16p0060.htm DB - PRIME DP - Unbound Medicine ER -