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Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube.
Andrology 2019; 7(2):228-234A

Abstract

BACKGROUND

Toll-like receptors play a crucial role in the immunological interaction between the spermatozoa and fallopian tube and contribute to the ovulation, sperm capacitation, fertilization, and pregnancy.

OBJECTIVES

To investigate the expression of toll-like receptors and their adaptor molecules and cytokines under the effect of spermatozoa with high DNA fragmentation (high DF) in human fallopian tube cell line (OE-E6/E7) and compare to those in normal spermatozoa.

MATERIALS AND METHODS

Fresh semen samples were obtained from 10 unexplained infertile males with high DF (more than 20%) and from 10 healthy donors with a DF less than 3%. After sperm preparation, samples were co-cultured with OE-E6/E7. Toll-like receptors, myeloid differentiation factor 88 (MyD88), TIR domain-containing adapter protein (TIRAP), TIR domain-containing adapter-inducing IFN-b (TRIF), TRIF-related adapter molecule as well as IL-6, IL-8, IFN-β, and TNFα mRNA expression were evaluated by quantitative real-time PCR. Protein levels of these cytokines and chemokines were measured using ELISA method.

RESULTS

TLR 1-6 mRNA expression in OE-E6/E7 was significantly higher under the effect of spermatozoa with high DF compared to the spermatozoa with low DF. Furthermore, significantly increased mRNA expression of MyD88, TIRAP, and TRIF was observed in the high DF group compared to the low DF group, except TRIF-related adapter molecule. Moreover, the expression of IL-6 and IL-8 in the high DF group was significantly higher than low DF group, although there was no significant difference in IFN-β and TNFα expression between the groups.

DISCUSSION AND CONCLUSION

Damage-associated molecular patterns from DNA damage activate TLR signaling pathway in human fallopian tubes and result in the upregulation of inflammatory cytokines and chemokines. This situation may provide pathologic environment for capacitation, fertilization, embryo development, and implantation in female reproductive tract and can be one of the mechanisms of infertility in men with high DF.

Authors+Show Affiliations

Cellular and Molecular Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Shahid Akbar Abadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences, Tehran, Iran.School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30663256

Citation

Zandieh, Z, et al. "Human Sperm DNA Damage Has an Effect On Immunological Interaction Between Spermatozoa and Fallopian Tube." Andrology, vol. 7, no. 2, 2019, pp. 228-234.
Zandieh Z, Ashrafi M, Aflatoonian K, et al. Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube. Andrology. 2019;7(2):228-234.
Zandieh, Z., Ashrafi, M., Aflatoonian, K., & Aflatoonian, R. (2019). Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube. Andrology, 7(2), pp. 228-234. doi:10.1111/andr.12574.
Zandieh Z, et al. Human Sperm DNA Damage Has an Effect On Immunological Interaction Between Spermatozoa and Fallopian Tube. Andrology. 2019;7(2):228-234. PubMed PMID: 30663256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube. AU - Zandieh,Z, AU - Ashrafi,M, AU - Aflatoonian,K, AU - Aflatoonian,R, Y1 - 2019/01/20/ PY - 2018/04/15/received PY - 2018/10/24/revised PY - 2018/11/07/accepted PY - 2019/1/22/pubmed PY - 2019/1/22/medline PY - 2019/1/22/entrez KW - fallopian tube KW - innate immunity KW - sperm DNA damage KW - toll-like receptors SP - 228 EP - 234 JF - Andrology JO - Andrology VL - 7 IS - 2 N2 - BACKGROUND: Toll-like receptors play a crucial role in the immunological interaction between the spermatozoa and fallopian tube and contribute to the ovulation, sperm capacitation, fertilization, and pregnancy. OBJECTIVES: To investigate the expression of toll-like receptors and their adaptor molecules and cytokines under the effect of spermatozoa with high DNA fragmentation (high DF) in human fallopian tube cell line (OE-E6/E7) and compare to those in normal spermatozoa. MATERIALS AND METHODS: Fresh semen samples were obtained from 10 unexplained infertile males with high DF (more than 20%) and from 10 healthy donors with a DF less than 3%. After sperm preparation, samples were co-cultured with OE-E6/E7. Toll-like receptors, myeloid differentiation factor 88 (MyD88), TIR domain-containing adapter protein (TIRAP), TIR domain-containing adapter-inducing IFN-b (TRIF), TRIF-related adapter molecule as well as IL-6, IL-8, IFN-β, and TNFα mRNA expression were evaluated by quantitative real-time PCR. Protein levels of these cytokines and chemokines were measured using ELISA method. RESULTS: TLR 1-6 mRNA expression in OE-E6/E7 was significantly higher under the effect of spermatozoa with high DF compared to the spermatozoa with low DF. Furthermore, significantly increased mRNA expression of MyD88, TIRAP, and TRIF was observed in the high DF group compared to the low DF group, except TRIF-related adapter molecule. Moreover, the expression of IL-6 and IL-8 in the high DF group was significantly higher than low DF group, although there was no significant difference in IFN-β and TNFα expression between the groups. DISCUSSION AND CONCLUSION: Damage-associated molecular patterns from DNA damage activate TLR signaling pathway in human fallopian tubes and result in the upregulation of inflammatory cytokines and chemokines. This situation may provide pathologic environment for capacitation, fertilization, embryo development, and implantation in female reproductive tract and can be one of the mechanisms of infertility in men with high DF. SN - 2047-2927 UR - https://www.unboundmedicine.com/medline/citation/30663256/Human_sperm_DNA_damage_has_an_effect_on_immunological_interaction_between_spermatozoa_and_fallopian_tube L2 - https://doi.org/10.1111/andr.12574 DB - PRIME DP - Unbound Medicine ER -