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A fluorescent probe composed of quantum dot labeled aptamer and graphene oxide for the determination of the lipopolysaccharide endotoxin.
Mikrochim Acta. 2019 01 21; 186(2):122.MA

Abstract

Endotoxins are complex lipopolysaccharides (LPS) and key components of the outer cell membrane of Gram-negative bacteria. The authors report on a fluorescent aptamer-based probe for the determination of LPS of Gram-negative bacteria. An aptamer against LPS was fluorescently labeled with CdSe/ZnS quantum dots. Its emission is quenched on addition of graphene oxide (GO). On addition of LPS, the aptamer binds LPS and GO is released. This results in the recovery of fluorescence, typically measured at excitation/emission wavelengths of 495/543 nm. The probe responds to LPS in the 10-500 ng·mL-1 concentration range, and the detection limit is 8.7 ng·mL-1. It can be used for selective detection of LPS from different Gram-negative bacteria, in the presence of biological interferents. Graphical abstract Schematic presentation of a green fluorescent probe comprised of an aptamer labelled with CdSe/ZnS quantum dots and of graphene oxide. Lipopolysaccharides bind to the aptamer and release graphene oxide to result in fluorescence recovery, which is measured at an emission wavelength 543 nm.

Authors+Show Affiliations

Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing University, Chongqing, 400030, China.Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. ljjcqu@126.com. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China. ljjcqu@126.com. Collaborative Innovation Center for Brain Science, Chongqing University, Chongqing, 400030, People's Republic of China. ljjcqu@126.com.Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing University, Chongqing, 400030, China.Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing University, Chongqing, 400030, China.Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing University, Chongqing, 400030, China.Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. xuyibbd@sina.com. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China. xuyibbd@sina.com. National Center for International Research of Micro/Nano-System and New Material Technology, Chongqing University, Chongqing, 400030, China. xuyibbd@sina.com. Collaborative Innovation Center for Brain Science, Chongqing University, Chongqing, 400030, People's Republic of China. xuyibbd@sina.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30666423

Citation

Wen, Lu-Xin, et al. "A Fluorescent Probe Composed of Quantum Dot Labeled Aptamer and Graphene Oxide for the Determination of the Lipopolysaccharide Endotoxin." Mikrochimica Acta, vol. 186, no. 2, 2019, p. 122.
Wen LX, Lv JJ, Chen L, et al. A fluorescent probe composed of quantum dot labeled aptamer and graphene oxide for the determination of the lipopolysaccharide endotoxin. Mikrochim Acta. 2019;186(2):122.
Wen, L. X., Lv, J. J., Chen, L., Li, S. B., Mou, X. J., & Xu, Y. (2019). A fluorescent probe composed of quantum dot labeled aptamer and graphene oxide for the determination of the lipopolysaccharide endotoxin. Mikrochimica Acta, 186(2), 122. https://doi.org/10.1007/s00604-018-3218-3
Wen LX, et al. A Fluorescent Probe Composed of Quantum Dot Labeled Aptamer and Graphene Oxide for the Determination of the Lipopolysaccharide Endotoxin. Mikrochim Acta. 2019 01 21;186(2):122. PubMed PMID: 30666423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fluorescent probe composed of quantum dot labeled aptamer and graphene oxide for the determination of the lipopolysaccharide endotoxin. AU - Wen,Lu-Xin, AU - Lv,Jun-Jiang, AU - Chen,Li, AU - Li,Shun-Bo, AU - Mou,Xiao-Jing, AU - Xu,Yi, Y1 - 2019/01/21/ PY - 2018/09/23/received PY - 2018/12/28/accepted PY - 2019/1/23/entrez PY - 2019/1/23/pubmed PY - 2019/2/5/medline KW - Biosensor KW - CdSe/ZnS quantum dots KW - DNA KW - Fluorescence quenching KW - Fluorescence turn-on KW - Gram-negative bacteria KW - Receptor KW - Resonance energy transfer SP - 122 EP - 122 JF - Mikrochimica acta JO - Mikrochim Acta VL - 186 IS - 2 N2 - Endotoxins are complex lipopolysaccharides (LPS) and key components of the outer cell membrane of Gram-negative bacteria. The authors report on a fluorescent aptamer-based probe for the determination of LPS of Gram-negative bacteria. An aptamer against LPS was fluorescently labeled with CdSe/ZnS quantum dots. Its emission is quenched on addition of graphene oxide (GO). On addition of LPS, the aptamer binds LPS and GO is released. This results in the recovery of fluorescence, typically measured at excitation/emission wavelengths of 495/543 nm. The probe responds to LPS in the 10-500 ng·mL-1 concentration range, and the detection limit is 8.7 ng·mL-1. It can be used for selective detection of LPS from different Gram-negative bacteria, in the presence of biological interferents. Graphical abstract Schematic presentation of a green fluorescent probe comprised of an aptamer labelled with CdSe/ZnS quantum dots and of graphene oxide. Lipopolysaccharides bind to the aptamer and release graphene oxide to result in fluorescence recovery, which is measured at an emission wavelength 543 nm. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30666423/A_fluorescent_probe_composed_of_quantum_dot_labeled_aptamer_and_graphene_oxide_for_the_determination_of_the_lipopolysaccharide_endotoxin_ DB - PRIME DP - Unbound Medicine ER -