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Comparative anti-arthritic investigation of iridoid glycosides and crocetin derivatives from Gardenia jasminoides Ellis in Freund's complete adjuvant-induced arthritis in rats.
Phytomedicine. 2019 Feb; 53:223-233.P

Abstract

BACKGROUND

Discovering novel compounds with higher activities is a key aim of natural products research. Gardenia jasminoides Ellis is a herb with anti-inflammatory properties. Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in this herb and are considered its active ingredients. However, which components are responsible for the anti-inflammatory properties of gardenia have remained to be investigated.

PURPOSE

Here, we prepared total iridoid glycocides (TIG) and total crocins (TC) from G. jasminoides Ellis, determined their main chemical constituents, and performed animal studies to evaluate their anti-adjuvant arthritis activities, thus, proposing a reasonable mechenism to explain the anti-inflammatory activities of the active components in this herbal remedy.

STUDY DESIGN

TIG and TC were prepared by using HPD-100 macroporous resin, and characterized by UHPLC-DAD-MS and UV-Vis spectrophotometer. Then, freund's complete adjuvant-injected rats underwent drug treatments with TIG (160 mg/kg) and TC (160 mg/kg) for 14 days, and their ankle diameters were measured. Moreover, X-ray radiographs of the adjuvant injected hind paws were evaluated. Finally, histopathological examinations of the ankle joints, spleens and thymus were carried out to evaluate inflammatory reactions, and immunohistochemical measurements were conducted to evaluate TNF-α and TGF-β1 expression in the ankle joint of the rats.

RESULTS

The chemical composition determination of the current study showed that TIG was mainly composed of geniposide and TC was a fraction predominantly with crocin-1, crocin-2 and crocin-3. Calculation of results showed that TIG and TC contained 58.2% total iridoid glycosides and 54.7% total crocins, respectively. Our study suggested TIG and TC treatments markedly decreased paw swelling and ankle diameters of AA rats (both p < 0.05). The radiological analysis showed that administration of TIG and TC ameliorated bone destruction, and reduced the radiological bone destruction scores (TIG p < 0.05, TC p>0.05). Moreover, data from histological assessment demonstrated considerable mitigation of inflammation in the joints (both p < 0.01), spleen and thymus of AA rats treated with TIG and TC. TNF-α and TGF-β1 protein expression according to immunohistochemistry staining also supported the anti-arthritis activities of TIG and TC (TNF-α: TIG p < 0.01 and TC p < 0.05, TGF-β1: TIG p < 0.01 and TC p>0.05).

CONCLUSION

In the current study, fractionation of gardenia prior to further in vivo investigation has for the first time provided reasonable explanation for the anti-inflammatory activity of this herbal remedy. Our study showed that both TIG and TC from gardenia have anti-inflammatory properties. Overall, these experimental findings suggest that gardenia could be regarded as a potential therapeutic target for arthritis. However, as geniposide has a higher content than crocins in this herbal drug, TIG (mainly geniposide) seems to be primarily responsible for the anti-inflammatory properties of gardenia. Taken together, this maiden attempt demonstrated that TIG (mainly geniposide) is more important in evaluating the anti-inflammatory activity of G. jasminoides Ellis.

Authors+Show Affiliations

Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China.West China School of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu, Sichuan, China.School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University Chenggong New City, Kunming, China. Electronic address: dengliangkmmc@163.com.Department of Biopharmaceuticals and Materials Engineering, Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine, Zunyi Medical University Zhuhai Campus, Zhuhai, China. Electronic address: chenyang197732@hotmail.com.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30668402

Citation

Hu, Yaya, et al. "Comparative Anti-arthritic Investigation of Iridoid Glycosides and Crocetin Derivatives From Gardenia Jasminoides Ellis in Freund's Complete Adjuvant-induced Arthritis in Rats." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 53, 2019, pp. 223-233.
Hu Y, Liu X, Xia Q, et al. Comparative anti-arthritic investigation of iridoid glycosides and crocetin derivatives from Gardenia jasminoides Ellis in Freund's complete adjuvant-induced arthritis in rats. Phytomedicine. 2019;53:223-233.
Hu, Y., Liu, X., Xia, Q., Yin, T., Bai, C., Wang, Z., Du, L., Li, X., Wang, W., Sun, L., Liu, Y., Zhang, H., Deng, L., & Chen, Y. (2019). Comparative anti-arthritic investigation of iridoid glycosides and crocetin derivatives from Gardenia jasminoides Ellis in Freund's complete adjuvant-induced arthritis in rats. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 53, 223-233. https://doi.org/10.1016/j.phymed.2018.07.005
Hu Y, et al. Comparative Anti-arthritic Investigation of Iridoid Glycosides and Crocetin Derivatives From Gardenia Jasminoides Ellis in Freund's Complete Adjuvant-induced Arthritis in Rats. Phytomedicine. 2019;53:223-233. PubMed PMID: 30668402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative anti-arthritic investigation of iridoid glycosides and crocetin derivatives from Gardenia jasminoides Ellis in Freund's complete adjuvant-induced arthritis in rats. AU - Hu,Yaya, AU - Liu,Xin, AU - Xia,Qiang, AU - Yin,Tianpeng, AU - Bai,Chuan, AU - Wang,Ze, AU - Du,Lianfeng, AU - Li,Xiaobo, AU - Wang,Wenjun, AU - Sun,Lin, AU - Liu,Yinhua, AU - Zhang,Hao, AU - Deng,Liang, AU - Chen,Yang, Y1 - 2018/07/25/ PY - 2017/11/12/received PY - 2018/05/08/revised PY - 2018/07/16/accepted PY - 2019/1/23/pubmed PY - 2019/7/16/medline PY - 2019/1/23/entrez KW - Adjuvant arthritic KW - Anti-inflammatory KW - Crocetin derivatives KW - Gardenia jasminoides Ellis KW - Geniposide KW - Iridoid glycosides SP - 223 EP - 233 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 53 N2 - BACKGROUND: Discovering novel compounds with higher activities is a key aim of natural products research. Gardenia jasminoides Ellis is a herb with anti-inflammatory properties. Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in this herb and are considered its active ingredients. However, which components are responsible for the anti-inflammatory properties of gardenia have remained to be investigated. PURPOSE: Here, we prepared total iridoid glycocides (TIG) and total crocins (TC) from G. jasminoides Ellis, determined their main chemical constituents, and performed animal studies to evaluate their anti-adjuvant arthritis activities, thus, proposing a reasonable mechenism to explain the anti-inflammatory activities of the active components in this herbal remedy. STUDY DESIGN: TIG and TC were prepared by using HPD-100 macroporous resin, and characterized by UHPLC-DAD-MS and UV-Vis spectrophotometer. Then, freund's complete adjuvant-injected rats underwent drug treatments with TIG (160 mg/kg) and TC (160 mg/kg) for 14 days, and their ankle diameters were measured. Moreover, X-ray radiographs of the adjuvant injected hind paws were evaluated. Finally, histopathological examinations of the ankle joints, spleens and thymus were carried out to evaluate inflammatory reactions, and immunohistochemical measurements were conducted to evaluate TNF-α and TGF-β1 expression in the ankle joint of the rats. RESULTS: The chemical composition determination of the current study showed that TIG was mainly composed of geniposide and TC was a fraction predominantly with crocin-1, crocin-2 and crocin-3. Calculation of results showed that TIG and TC contained 58.2% total iridoid glycosides and 54.7% total crocins, respectively. Our study suggested TIG and TC treatments markedly decreased paw swelling and ankle diameters of AA rats (both p < 0.05). The radiological analysis showed that administration of TIG and TC ameliorated bone destruction, and reduced the radiological bone destruction scores (TIG p < 0.05, TC p>0.05). Moreover, data from histological assessment demonstrated considerable mitigation of inflammation in the joints (both p < 0.01), spleen and thymus of AA rats treated with TIG and TC. TNF-α and TGF-β1 protein expression according to immunohistochemistry staining also supported the anti-arthritis activities of TIG and TC (TNF-α: TIG p < 0.01 and TC p < 0.05, TGF-β1: TIG p < 0.01 and TC p>0.05). CONCLUSION: In the current study, fractionation of gardenia prior to further in vivo investigation has for the first time provided reasonable explanation for the anti-inflammatory activity of this herbal remedy. Our study showed that both TIG and TC from gardenia have anti-inflammatory properties. Overall, these experimental findings suggest that gardenia could be regarded as a potential therapeutic target for arthritis. However, as geniposide has a higher content than crocins in this herbal drug, TIG (mainly geniposide) seems to be primarily responsible for the anti-inflammatory properties of gardenia. Taken together, this maiden attempt demonstrated that TIG (mainly geniposide) is more important in evaluating the anti-inflammatory activity of G. jasminoides Ellis. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/30668402/Comparative_anti_arthritic_investigation_of_iridoid_glycosides_and_crocetin_derivatives_from_Gardenia_jasminoides_Ellis_in_Freund's_complete_adjuvant_induced_arthritis_in_rats_ DB - PRIME DP - Unbound Medicine ER -