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Isoliquiritigenin exerts antioxidant activity in Caenorhabditis elegans via insulin-like signaling pathway and SKN-1.
Phytomedicine. 2019 Mar 01; 55:119-124.P

Abstract

BACKGROUND

Glycyrrhiza uralensis is a well-known medicinal plant. Different therapeutic effects have been reported for its secondary metabolites, including neuroprotective activity. Antioxidant properties have also been documented for some of its compounds and it could be a possible mechanism of neuroprotection.

PURPOSE

The present study was conducted to investigate the antioxidant effect and underlying pathways of G. uralensis and its main compounds.

METHODS

The experiments were conducted with Caenorhabditis elegans, a simple in vivo model, widely used in this context. The methanol extract of G. uralensis and its main compounds isoliquiritigenin, liquiritigenin, glycyrrhizic acid, and glycyrrhetinic acid were tested for their effects on heat shock protein expression under mild oxidative stress and survival rate under lethal oxidative stress. To clarify the underlying pathways, the effect on the transcription factors DAF-16, SKN-1, and HSF-1 was tested.

RESULTS

Isoliquiritigenin was the most potent compound in both assays, leading to a 31% decrease in expression of the stress marker heat shock protein and an 87% increase in survival rate. It significantly activated DAF-16 and SKN-1, but not HSF-1.

CONCLUSION

The present study identified isoliquiritigenin as the most active antioxidant compound in G. uralensis. It exerts its effect by activating the transcription factors DAF-16/FOXO and SKN-1/Nrf2 which regulate many genes, including those which code for proteins of antioxidative response. This implicates isoliquiritigenin as a possible supplement drug against oxidative stress especially in neurodegenerative diseases.

Authors+Show Affiliations

Department of Biology, Institute of Pharmacy and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg, Germany. Electronic address: Link@uni-heidelberg.de.Department of Biology, Institute of Pharmacy and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg, Germany. Electronic address: wink@uni-heidelberg.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30668421

Citation

Link, Pille, and Michael Wink. "Isoliquiritigenin Exerts Antioxidant Activity in Caenorhabditis Elegans Via Insulin-like Signaling Pathway and SKN-1." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 55, 2019, pp. 119-124.
Link P, Wink M. Isoliquiritigenin exerts antioxidant activity in Caenorhabditis elegans via insulin-like signaling pathway and SKN-1. Phytomedicine. 2019;55:119-124.
Link, P., & Wink, M. (2019). Isoliquiritigenin exerts antioxidant activity in Caenorhabditis elegans via insulin-like signaling pathway and SKN-1. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 55, 119-124. https://doi.org/10.1016/j.phymed.2018.07.004
Link P, Wink M. Isoliquiritigenin Exerts Antioxidant Activity in Caenorhabditis Elegans Via Insulin-like Signaling Pathway and SKN-1. Phytomedicine. 2019 Mar 1;55:119-124. PubMed PMID: 30668421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoliquiritigenin exerts antioxidant activity in Caenorhabditis elegans via insulin-like signaling pathway and SKN-1. AU - Link,Pille, AU - Wink,Michael, Y1 - 2018/07/20/ PY - 2017/11/07/received PY - 2018/06/05/revised PY - 2018/07/14/accepted PY - 2019/1/23/pubmed PY - 2019/6/25/medline PY - 2019/1/23/entrez KW - Antioxidant activity KW - Caenorhabditis elegans KW - DAF-16/FOXO KW - Glycyrrhiza uralensis KW - Isoliquiritigenin KW - SKN-1/Nrf2 SP - 119 EP - 124 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 55 N2 - BACKGROUND: Glycyrrhiza uralensis is a well-known medicinal plant. Different therapeutic effects have been reported for its secondary metabolites, including neuroprotective activity. Antioxidant properties have also been documented for some of its compounds and it could be a possible mechanism of neuroprotection. PURPOSE: The present study was conducted to investigate the antioxidant effect and underlying pathways of G. uralensis and its main compounds. METHODS: The experiments were conducted with Caenorhabditis elegans, a simple in vivo model, widely used in this context. The methanol extract of G. uralensis and its main compounds isoliquiritigenin, liquiritigenin, glycyrrhizic acid, and glycyrrhetinic acid were tested for their effects on heat shock protein expression under mild oxidative stress and survival rate under lethal oxidative stress. To clarify the underlying pathways, the effect on the transcription factors DAF-16, SKN-1, and HSF-1 was tested. RESULTS: Isoliquiritigenin was the most potent compound in both assays, leading to a 31% decrease in expression of the stress marker heat shock protein and an 87% increase in survival rate. It significantly activated DAF-16 and SKN-1, but not HSF-1. CONCLUSION: The present study identified isoliquiritigenin as the most active antioxidant compound in G. uralensis. It exerts its effect by activating the transcription factors DAF-16/FOXO and SKN-1/Nrf2 which regulate many genes, including those which code for proteins of antioxidative response. This implicates isoliquiritigenin as a possible supplement drug against oxidative stress especially in neurodegenerative diseases. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/30668421/Isoliquiritigenin_exerts_antioxidant_activity_in_Caenorhabditis_elegans_via_insulin_like_signaling_pathway_and_SKN_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(18)30256-3 DB - PRIME DP - Unbound Medicine ER -