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Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose.
Dev Comp Immunol. 2019 05; 94:59-65.DC

Abstract

The present study was conducted to evaluate the effects of dietary Pediococcus acidilactici (PA) and raffinose (RF) alone or in combination on growth performance, mucosal immune factors and immune related genes expression in common carp (Cyprinus carpio) juveniles. Fish with initial weight of 10.0 ± 2.5 g were fed the following experimental diets for 60 days: control (without supplementation), prebiotic (2 g RF kg-1 diet), probiotic (6 × 108 CFU g-1PA) and synbiotic (2 g RF kg-1+ 6 × 108 CFU g-1PA). Carp fed synbiotic and probiotic diets had the highest (19.53 ± 0.16) and the lowest (18.05 ± 0.65) final body weight, respectively and the other experimental groups showed intermediate values. Singular administration of PA or in combination with 2 g RF kg-1 significantly increased skin mucus total immunoglobulin (Ig) and protein compared other groups, meanwhile, values of skin mucus protease activity enhanced by dietary immunostimulants administration in comparison with the control (P < 0.05). The expression of gene encoding lysozyme in skin pronouncedly increased by supplementing diets with singular or mixture of PA and RF; however, the expression of intestinal lysozyme gene as well as tumor necrosis factor-α genes expression in skin and intestine were not affected by administrating different immunostimulants (P > 0.05). The highest growth performance was noticed in fish fed synbiotic (P < 0.05). Overall, the combination of 2 g RF kg-1 with 6 × 108 CFU g-1PA is recommended for improving immunological responses of C. carpio juveniles.

Authors+Show Affiliations

Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Veterinary Medicine, Peoples' Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow 117198, Russian Federation.Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: hien.d@cmu.ac.th.South Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Ahwaz, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30668960

Citation

Hoseinifar, Seyed Hossein, et al. "Enhanced Mucosal Immune Responses, Immune Related Genes and Growth Performance in Common Carp (Cyprinus Carpio) Juveniles Fed Dietary Pediococcus Acidilactici MA18/5M and Raffinose." Developmental and Comparative Immunology, vol. 94, 2019, pp. 59-65.
Hoseinifar SH, Hosseini M, Paknejad H, et al. Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose. Dev Comp Immunol. 2019;94:59-65.
Hoseinifar, S. H., Hosseini, M., Paknejad, H., Safari, R., Jafar, A., Yousefi, M., Van Doan, H., & Torfi Mozanzadeh, M. (2019). Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose. Developmental and Comparative Immunology, 94, 59-65. https://doi.org/10.1016/j.dci.2019.01.009
Hoseinifar SH, et al. Enhanced Mucosal Immune Responses, Immune Related Genes and Growth Performance in Common Carp (Cyprinus Carpio) Juveniles Fed Dietary Pediococcus Acidilactici MA18/5M and Raffinose. Dev Comp Immunol. 2019;94:59-65. PubMed PMID: 30668960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose. AU - Hoseinifar,Seyed Hossein, AU - Hosseini,Marjan, AU - Paknejad,Hamed, AU - Safari,Roghieh, AU - Jafar,Ali, AU - Yousefi,Morteza, AU - Van Doan,Hien, AU - Torfi Mozanzadeh,Mansour, Y1 - 2019/01/19/ PY - 2018/12/07/received PY - 2019/01/18/revised PY - 2019/01/18/accepted PY - 2019/1/23/pubmed PY - 2019/8/14/medline PY - 2019/1/23/entrez KW - Common carp KW - Cytokines KW - Lactic acid bacteria KW - Raffinose KW - Skin mucosal immunity KW - Synbiotics SP - 59 EP - 65 JF - Developmental and comparative immunology JO - Dev Comp Immunol VL - 94 N2 - The present study was conducted to evaluate the effects of dietary Pediococcus acidilactici (PA) and raffinose (RF) alone or in combination on growth performance, mucosal immune factors and immune related genes expression in common carp (Cyprinus carpio) juveniles. Fish with initial weight of 10.0 ± 2.5 g were fed the following experimental diets for 60 days: control (without supplementation), prebiotic (2 g RF kg-1 diet), probiotic (6 × 108 CFU g-1PA) and synbiotic (2 g RF kg-1+ 6 × 108 CFU g-1PA). Carp fed synbiotic and probiotic diets had the highest (19.53 ± 0.16) and the lowest (18.05 ± 0.65) final body weight, respectively and the other experimental groups showed intermediate values. Singular administration of PA or in combination with 2 g RF kg-1 significantly increased skin mucus total immunoglobulin (Ig) and protein compared other groups, meanwhile, values of skin mucus protease activity enhanced by dietary immunostimulants administration in comparison with the control (P < 0.05). The expression of gene encoding lysozyme in skin pronouncedly increased by supplementing diets with singular or mixture of PA and RF; however, the expression of intestinal lysozyme gene as well as tumor necrosis factor-α genes expression in skin and intestine were not affected by administrating different immunostimulants (P > 0.05). The highest growth performance was noticed in fish fed synbiotic (P < 0.05). Overall, the combination of 2 g RF kg-1 with 6 × 108 CFU g-1PA is recommended for improving immunological responses of C. carpio juveniles. SN - 1879-0089 UR - https://www.unboundmedicine.com/medline/citation/30668960/Enhanced_mucosal_immune_responses_immune_related_genes_and_growth_performance_in_common_carp__Cyprinus_carpio__juveniles_fed_dietary_Pediococcus_acidilactici_MA18/5M_and_raffinose_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(18)30586-X DB - PRIME DP - Unbound Medicine ER -