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Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus.
Phytother Res. 2019 Apr; 33(4):1010-1018.PR

Abstract

Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.

Authors+Show Affiliations

Department of Microbiology and Biotechnology, Szent István University, Budapest, Hungary.Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.Department of Microbiology and Biotechnology, Szent István University, Budapest, Hungary.Department of Microbiology and Biotechnology, Szent István University, Budapest, Hungary.Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30672036

Citation

Mouwakeh, Ahmad, et al. "Nigella Sativa Essential Oil and Its Bioactive Compounds as Resistance Modifiers Against Staphylococcus Aureus." Phytotherapy Research : PTR, vol. 33, no. 4, 2019, pp. 1010-1018.
Mouwakeh A, Kincses A, Nové M, et al. Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. Phytother Res. 2019;33(4):1010-1018.
Mouwakeh, A., Kincses, A., Nové, M., Mosolygó, T., Mohácsi-Farkas, C., Kiskó, G., & Spengler, G. (2019). Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. Phytotherapy Research : PTR, 33(4), 1010-1018. https://doi.org/10.1002/ptr.6294
Mouwakeh A, et al. Nigella Sativa Essential Oil and Its Bioactive Compounds as Resistance Modifiers Against Staphylococcus Aureus. Phytother Res. 2019;33(4):1010-1018. PubMed PMID: 30672036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. AU - Mouwakeh,Ahmad, AU - Kincses,Annamária, AU - Nové,Márta, AU - Mosolygó,Tímea, AU - Mohácsi-Farkas,Csilla, AU - Kiskó,Gabriella, AU - Spengler,Gabriella, Y1 - 2019/01/23/ PY - 2018/07/23/received PY - 2018/12/04/revised PY - 2018/12/31/accepted PY - 2019/1/24/pubmed PY - 2019/6/14/medline PY - 2019/1/24/entrez KW - Nigella sativa essential oil KW - Staphylococcus aureus KW - antibacterial KW - biofilm KW - efflux pump inhibitor (EPI) SP - 1010 EP - 1018 JF - Phytotherapy research : PTR JO - Phytother Res VL - 33 IS - 4 N2 - Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/30672036/Nigella_sativa_essential_oil_and_its_bioactive_compounds_as_resistance_modifiers_against_Staphylococcus_aureus_ L2 - https://doi.org/10.1002/ptr.6294 DB - PRIME DP - Unbound Medicine ER -