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Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China.
Plant Dis 2017; 101(4):557-561PD

Abstract

The blast (Magnaporthe oryzae) resistance (R) gene is the most economical and environmental method to control rice blast disease. Characterization of molecular identity and pathogenicity of M. oryzae benefits the deployment of effective blast R genes. In order to identify blast R genes that would be effective in Hunan Province,182 M. oryzae strains were analyzed with a Chinese differential system (CDS), repetitive element-based polymerase chain reaction (rep-PCR), and the presence and absence of avirulence (AVR) genes by PCR amplification with gene-specific primers. Identified blast R genes were validated with 24 monogenic lines (ML) carrying 24 major R genes. In total, 28 races (isolates) of M. oryzae was identified with CDS, and classified into 20 distinct groups with rep-PCR. Interestingly, AVR-Pia, AVR-Pik, AVR-Pizt, AVR-Pib, and AVR-Pi9 were detected in more than 86.8% of the isolates; AVR-Pita1 was in 51.3% and AVR-Pii was in only 2.5%. In contrast, pathogenicity assays on 24 ML demonstrated that Pi9, Piz5, Pikh, and Pikm were more effective, with resistant frequencies of 91.6, 91, 87.9, and 87.3%, respectively; Pia, Piks, Pit, Pi12, and Pib were less than 15%. These findings revealed the complexity of a genetic basis of rice blast resistance, and shed light on useful blast R genes in Hunan Province.

Authors+Show Affiliations

State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China.United States Department of Agriculture-Agricultural Research Service, Dale Bumpers National Rice Research Center, Stuttgart, AR 72160.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center.Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, and Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha 410128, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30677362

Citation

Xing, Junjie, et al. "Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China." Plant Disease, vol. 101, no. 4, 2017, pp. 557-561.
Xing J, Jia Y, Peng Z, et al. Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China. Plant Dis. 2017;101(4):557-561.
Xing, J., Jia, Y., Peng, Z., Shi, Y., He, Q., Shu, F., ... Deng, H. (2017). Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China. Plant Disease, 101(4), pp. 557-561. doi:10.1094/PDIS-03-16-0288-RE.
Xing J, et al. Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China. Plant Dis. 2017;101(4):557-561. PubMed PMID: 30677362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China. AU - Xing,Junjie, AU - Jia,Yulin, AU - Peng,Zhirong, AU - Shi,Yinfeng, AU - He,Qiang, AU - Shu,Fu, AU - Zhang,Wuhan, AU - Zhang,Zhen, AU - Deng,Huafeng, Y1 - 2017/01/27/ PY - 2019/1/25/entrez PY - 2017/4/1/pubmed PY - 2017/4/1/medline SP - 557 EP - 561 JF - Plant disease JO - Plant Dis. VL - 101 IS - 4 N2 - The blast (Magnaporthe oryzae) resistance (R) gene is the most economical and environmental method to control rice blast disease. Characterization of molecular identity and pathogenicity of M. oryzae benefits the deployment of effective blast R genes. In order to identify blast R genes that would be effective in Hunan Province,182 M. oryzae strains were analyzed with a Chinese differential system (CDS), repetitive element-based polymerase chain reaction (rep-PCR), and the presence and absence of avirulence (AVR) genes by PCR amplification with gene-specific primers. Identified blast R genes were validated with 24 monogenic lines (ML) carrying 24 major R genes. In total, 28 races (isolates) of M. oryzae was identified with CDS, and classified into 20 distinct groups with rep-PCR. Interestingly, AVR-Pia, AVR-Pik, AVR-Pizt, AVR-Pib, and AVR-Pi9 were detected in more than 86.8% of the isolates; AVR-Pita1 was in 51.3% and AVR-Pii was in only 2.5%. In contrast, pathogenicity assays on 24 ML demonstrated that Pi9, Piz5, Pikh, and Pikm were more effective, with resistant frequencies of 91.6, 91, 87.9, and 87.3%, respectively; Pia, Piks, Pit, Pi12, and Pib were less than 15%. These findings revealed the complexity of a genetic basis of rice blast resistance, and shed light on useful blast R genes in Hunan Province. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/30677362/Characterization_of_Molecular_Identity_and_Pathogenicity_of_Rice_Blast_Fungus_in_Hunan_Province_of_China_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-03-16-0288-RE?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -