Citation
Hodille, Elisabeth, et al. "Evaluation of Xpert MTB/RIF Ultra Performance for Pulmonary Tuberculosis Diagnosis On Smear-negative Respiratory Samples in a French Centre." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 38, no. 3, 2019, pp. 601-605.
Hodille E, Maisson A, Charlet L, et al. Evaluation of Xpert MTB/RIF Ultra performance for pulmonary tuberculosis diagnosis on smear-negative respiratory samples in a French centre. Eur J Clin Microbiol Infect Dis. 2019;38(3):601-605.
Hodille, E., Maisson, A., Charlet, L., Bauduin, C., Genestet, C., Fredenucci, I., Rasigade, J. P., Lina, G., & Dumitrescu, O. (2019). Evaluation of Xpert MTB/RIF Ultra performance for pulmonary tuberculosis diagnosis on smear-negative respiratory samples in a French centre. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 38(3), 601-605. https://doi.org/10.1007/s10096-018-03463-1
Hodille E, et al. Evaluation of Xpert MTB/RIF Ultra Performance for Pulmonary Tuberculosis Diagnosis On Smear-negative Respiratory Samples in a French Centre. Eur J Clin Microbiol Infect Dis. 2019;38(3):601-605. PubMed PMID: 30680567.
TY - JOUR
T1 - Evaluation of Xpert MTB/RIF Ultra performance for pulmonary tuberculosis diagnosis on smear-negative respiratory samples in a French centre.
AU - Hodille,Elisabeth,
AU - Maisson,Audey,
AU - Charlet,Laurine,
AU - Bauduin,Clyde,
AU - Genestet,Charlotte,
AU - Fredenucci,Isabelle,
AU - Rasigade,Jean-Philippe,
AU - Lina,Gérard,
AU - Dumitrescu,Oana,
Y1 - 2019/01/24/
PY - 2018/11/21/received
PY - 2018/12/26/accepted
PY - 2019/1/27/pubmed
PY - 2019/9/13/medline
PY - 2019/1/26/entrez
KW - Diagnosis
KW - High-resource setting
KW - Pulmonary tuberculosis
KW - Smear-negative
KW - Xpert MTB/RIF Ultra
SP - 601
EP - 605
JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
JO - Eur J Clin Microbiol Infect Dis
VL - 38
IS - 3
N2 - Tuberculosis (TB) is a worldwide public health concern, including in high-resource countries with a low prevalence of TB. Xpert MTB/RIF assay was developed to improve TB and rifampicin (RIF) resistance detection, but sensitivity remains poor on smear-negative sputum. Xpert MTB/RIF Ultra assay was designed to enhance the sensitivity of TB detection in clinical samples. Herein, we evaluated retrospectively the performance of this test on smear-negative respiratory samples. Respiratory specimens with smear-negative and a Mycobacterium tuberculosis (MTB) complex-positive culture were retrospectively selected from those taken from patients during routine care, and analysed in the Mycobacteria Laboratory of the Lyon University hospital, France. Specimens were stored at - 20 °C before testing by Xpert MTB/RIF Ultra. For each sample, growth delay and date of anti-TB treatment initiation were recorded. Forty-six samples-29 sputum, 8 bronchial aspirates, 6 broncho-alveolar lavages, and 3 gastric aspirates-were selected. Among samples collected before treatment initiation (n = 33), sensitivity was 81.8% (95% CI [64.5; 93.0]) and there was a significant correlation between the quantitative measurements (Ct) of Xpert MTB/RIF Ultra assay and the time to growth detection in culture. Among samples collected after treatment initiation (n = 12), sensitivity was 100%, without correlation with time to growth detection due to presence of afterglow DNA in samples. In high-resource settings, the Xpert MTB/RIF Ultra test represents a useful tool for pulmonary TB diagnosis, notably for the paucibacillary forms. Moreover, quantitative measurement of Xpert MTB/RIF Ultra could help to predict time to MTB culture positivity and be used as a quality indicator of MTB culture process.
SN - 1435-4373
UR - https://www.unboundmedicine.com/medline/citation/30680567/Evaluation_of_Xpert_MTB/RIF_Ultra_performance_for_pulmonary_tuberculosis_diagnosis_on_smear_negative_respiratory_samples_in_a_French_centre_
DB - PRIME
DP - Unbound Medicine
ER -