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Electrochemiluminecence nanogears aptasensor based on MIL-53(Fe)@CdS for multiplexed detection of kanamycin and neomycin.
Biosens Bioelectron. 2019 Mar 15; 129:100-106.BB

Abstract

A dual gears electrochemiluminecence (ECL) aptasensing strategy for multiple selective determination of kanamycin and neocycin was designed on the basis of the combination of kanamycin and neocycin induced dual gears conversion, the loading platform of metal-organic frameworks (MOFs), surface plasmon resonance (SPR) and ECL resonance energy transfer (ERET) between CdS QDs and AuNPs (or PtNPs). In the absence of target, the dual gears were "off". Then the B1-AuNP (gear B) and aptamer 1-PtNPs acted as signal quenching elements to quench ECL intensity due to ERET process. Upon addition of kanamycin, the aptamer 1-PtNPs were removed from the gear gradually, the ECL was enhanced due to SPR process between AuNPs and CdS QDs. After the incubation of aptamer 2, the dual gears were "off" again and ECL intensity was decreased by ERET process between AuNPs and CdS QDs. In the presence of neomycin, dual gears were "on" again, the ECL signal was enhanced by SPR process between AuNPs and CdS QDs. Under optimal condition, the proposed aptasensor exhibited wide linear ranges of kanamycin (10-10-10-6 M) and neomycin (10-9-10-5 M), and relatively low detection limits to kanamycin (1.7 × 10-11 M) and neomycin (3.5 × 10-10 M). The developed aptasensor realized the multiple ECL detection of kanamycin and neomycin with single luminophore, and was successfully applied to the detection of kanamycin and neomycin in food samples.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning 530008, China.School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning 530008, China. Electronic address: gxunxctan@126.com.School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning 530008, China. Electronic address: eveyeyu@163.com.School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning 530008, China.School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning 530008, China.School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning 530008, China.State Key Laboratory of Agricultural Microbiology, College of Science, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

30685704

Citation

Feng, Defen, et al. "Electrochemiluminecence Nanogears Aptasensor Based On MIL-53(Fe)@CdS for Multiplexed Detection of Kanamycin and Neomycin." Biosensors & Bioelectronics, vol. 129, 2019, pp. 100-106.
Feng D, Tan X, Wu Y, et al. Electrochemiluminecence nanogears aptasensor based on MIL-53(Fe)@CdS for multiplexed detection of kanamycin and neomycin. Biosens Bioelectron. 2019;129:100-106.
Feng, D., Tan, X., Wu, Y., Ai, C., Luo, Y., Chen, Q., & Han, H. (2019). Electrochemiluminecence nanogears aptasensor based on MIL-53(Fe)@CdS for multiplexed detection of kanamycin and neomycin. Biosensors & Bioelectronics, 129, 100-106. https://doi.org/10.1016/j.bios.2018.12.050
Feng D, et al. Electrochemiluminecence Nanogears Aptasensor Based On MIL-53(Fe)@CdS for Multiplexed Detection of Kanamycin and Neomycin. Biosens Bioelectron. 2019 Mar 15;129:100-106. PubMed PMID: 30685704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemiluminecence nanogears aptasensor based on MIL-53(Fe)@CdS for multiplexed detection of kanamycin and neomycin. AU - Feng,Defen, AU - Tan,Xuecai, AU - Wu,Yeyu, AU - Ai,Chenhao, AU - Luo,Yanni, AU - Chen,Quanyou, AU - Han,Heyou, Y1 - 2019/01/09/ PY - 2018/10/27/received PY - 2018/12/19/revised PY - 2018/12/28/accepted PY - 2019/1/28/pubmed PY - 2019/6/25/medline PY - 2019/1/28/entrez KW - Antibiotic KW - Electrochemiluminecence resonance energy transfer KW - Electrochemiluminescence KW - Nanogears KW - Surface plasmon resonance SP - 100 EP - 106 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 129 N2 - A dual gears electrochemiluminecence (ECL) aptasensing strategy for multiple selective determination of kanamycin and neocycin was designed on the basis of the combination of kanamycin and neocycin induced dual gears conversion, the loading platform of metal-organic frameworks (MOFs), surface plasmon resonance (SPR) and ECL resonance energy transfer (ERET) between CdS QDs and AuNPs (or PtNPs). In the absence of target, the dual gears were "off". Then the B1-AuNP (gear B) and aptamer 1-PtNPs acted as signal quenching elements to quench ECL intensity due to ERET process. Upon addition of kanamycin, the aptamer 1-PtNPs were removed from the gear gradually, the ECL was enhanced due to SPR process between AuNPs and CdS QDs. After the incubation of aptamer 2, the dual gears were "off" again and ECL intensity was decreased by ERET process between AuNPs and CdS QDs. In the presence of neomycin, dual gears were "on" again, the ECL signal was enhanced by SPR process between AuNPs and CdS QDs. Under optimal condition, the proposed aptasensor exhibited wide linear ranges of kanamycin (10-10-10-6 M) and neomycin (10-9-10-5 M), and relatively low detection limits to kanamycin (1.7 × 10-11 M) and neomycin (3.5 × 10-10 M). The developed aptasensor realized the multiple ECL detection of kanamycin and neomycin with single luminophore, and was successfully applied to the detection of kanamycin and neomycin in food samples. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/30685704/Electrochemiluminecence_nanogears_aptasensor_based_on_MIL_53_Fe_@CdS_for_multiplexed_detection_of_kanamycin_and_neomycin_ DB - PRIME DP - Unbound Medicine ER -