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Trps1 transcription factor regulates mineralization of dental tissues and proliferation of tooth organ cells.
Mol Genet Metab. 2019 04; 126(4):504-512.MG

Abstract

Mutations of the TRPS1 gene cause trichorhinophalangeal syndrome (TRPS), a skeletal dysplasia with dental abnormalities. TRPS dental phenotypes suggest that TRPS1 regulates multiple aspects of odontogenesis, including the tooth number and size. Previous studies delineating Trps1 expression throughout embryonic tooth development in mice detected strong Trps1 expression in dental mesenchyme, preodontoblasts, and dental follicles, suggesting that TRPS dental phenotypes result from abnormalities in early developmental processes. In this study, Trps1+/- and Trps1-/- mice were analyzed to determine consequences of Trps1 deficiency on odontogenesis. We focused on the aspects of tooth formation that are disturbed in TRPS and on potential molecular abnormalities underlying TRPS dental phenotypes. Microcomputed tomography analyses of molars were used to determine tooth size, crown shape, and mineralization of dental tissues. These analyses uncovered that disruption of one Trps1 allele is sufficient to impair mineralization of dentin in both male and female mice. Enamel mineral density was decreased only in males, while mineralization of the root dental tissues was decreased only in females. In addition, significantly smaller teeth were detected in Trps1+/- females. Histomorphometric analyses of tooth organs showed reduced anterior-posterior diameter in Trps1-/- mice. BrdU-incorporation assay detected reduced proliferation of mesenchymal and epithelial cells in Trps1-/- tooth organs. Immunohistochemistry for Runx2 and Osx osteogenic transcription factors revealed changes in their spatial distribution in Trps1-/- tooth organs and uncovered cell-type specific requirements of Trps1 for Osx expression. In conclusion, this study has demonstrated that Trps1 is a positive regulator of cell proliferation in both dental mesenchyme and epithelium, suggesting that the microdontia in TRPS is likely due to decreased cell proliferation in developing tooth organs. Furthermore, the reduced mineralization observed in Trps1+/- mice may provide some explanation for the extensive dental caries reported in TRPS patients.

Authors+Show Affiliations

Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, AL, USA.Center for Craniofacial Regeneration, Dept. of Oral Biology, McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.Center for Craniofacial Regeneration, Dept. of Oral Biology, McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.Center for Craniofacial Regeneration, Dept. of Oral Biology, McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.Center for Craniofacial Regeneration, Dept. of Oral Biology, McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA. Electronic address: don11@pitt.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30691926

Citation

Goss, Morgan, et al. "Trps1 Transcription Factor Regulates Mineralization of Dental Tissues and Proliferation of Tooth Organ Cells." Molecular Genetics and Metabolism, vol. 126, no. 4, 2019, pp. 504-512.
Goss M, Socorro M, Monier D, et al. Trps1 transcription factor regulates mineralization of dental tissues and proliferation of tooth organ cells. Mol Genet Metab. 2019;126(4):504-512.
Goss, M., Socorro, M., Monier, D., Verdelis, K., & Napierala, D. (2019). Trps1 transcription factor regulates mineralization of dental tissues and proliferation of tooth organ cells. Molecular Genetics and Metabolism, 126(4), 504-512. https://doi.org/10.1016/j.ymgme.2019.01.014
Goss M, et al. Trps1 Transcription Factor Regulates Mineralization of Dental Tissues and Proliferation of Tooth Organ Cells. Mol Genet Metab. 2019;126(4):504-512. PubMed PMID: 30691926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trps1 transcription factor regulates mineralization of dental tissues and proliferation of tooth organ cells. AU - Goss,Morgan, AU - Socorro,Mairobys, AU - Monier,Daisy, AU - Verdelis,Kostas, AU - Napierala,Dobrawa, Y1 - 2019/01/23/ PY - 2018/08/20/received PY - 2019/01/17/revised PY - 2019/01/18/accepted PY - 2020/04/01/pmc-release PY - 2019/1/30/pubmed PY - 2019/11/8/medline PY - 2019/1/30/entrez KW - Cell proliferation KW - Mineralization KW - Tooth development KW - Transcription factor KW - Trichorhinophalangeal syndrome SP - 504 EP - 512 JF - Molecular genetics and metabolism JO - Mol. Genet. Metab. VL - 126 IS - 4 N2 - Mutations of the TRPS1 gene cause trichorhinophalangeal syndrome (TRPS), a skeletal dysplasia with dental abnormalities. TRPS dental phenotypes suggest that TRPS1 regulates multiple aspects of odontogenesis, including the tooth number and size. Previous studies delineating Trps1 expression throughout embryonic tooth development in mice detected strong Trps1 expression in dental mesenchyme, preodontoblasts, and dental follicles, suggesting that TRPS dental phenotypes result from abnormalities in early developmental processes. In this study, Trps1+/- and Trps1-/- mice were analyzed to determine consequences of Trps1 deficiency on odontogenesis. We focused on the aspects of tooth formation that are disturbed in TRPS and on potential molecular abnormalities underlying TRPS dental phenotypes. Microcomputed tomography analyses of molars were used to determine tooth size, crown shape, and mineralization of dental tissues. These analyses uncovered that disruption of one Trps1 allele is sufficient to impair mineralization of dentin in both male and female mice. Enamel mineral density was decreased only in males, while mineralization of the root dental tissues was decreased only in females. In addition, significantly smaller teeth were detected in Trps1+/- females. Histomorphometric analyses of tooth organs showed reduced anterior-posterior diameter in Trps1-/- mice. BrdU-incorporation assay detected reduced proliferation of mesenchymal and epithelial cells in Trps1-/- tooth organs. Immunohistochemistry for Runx2 and Osx osteogenic transcription factors revealed changes in their spatial distribution in Trps1-/- tooth organs and uncovered cell-type specific requirements of Trps1 for Osx expression. In conclusion, this study has demonstrated that Trps1 is a positive regulator of cell proliferation in both dental mesenchyme and epithelium, suggesting that the microdontia in TRPS is likely due to decreased cell proliferation in developing tooth organs. Furthermore, the reduced mineralization observed in Trps1+/- mice may provide some explanation for the extensive dental caries reported in TRPS patients. SN - 1096-7206 UR - https://www.unboundmedicine.com/medline/citation/30691926/Trps1_transcription_factor_regulates_mineralization_of_dental_tissues_and_proliferation_of_tooth_organ_cells L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-7192(18)30506-7 DB - PRIME DP - Unbound Medicine ER -