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Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain.
Microb Ecol. 2019 Aug; 78(2):409-415.ME

Abstract

The aim of this study was to determine the carriage rate of coagulase-positive staphylococci (CoPS) in wild birds and to characterize recovered isolates. Tracheal samples from 324 wild birds, obtained in different Spanish regions during 2015-2016, were screened for CoPS carriage. The antimicrobial resistance profile and the virulence gene content were investigated. Molecular typing was performed by spa, agr, MLST, SCCmec, and S. delphini group classification. CoPS were recovered from 26 samples of wild birds (8.3%), and 27 isolates were further characterized. Two CoPS species were detected: S. aureus (n = 15; eight cinereous vultures and seven magpies) and S. delphini (n = 12; 11 cinereous vultures and one red kite). Thirteen S. aureus were methicillin-resistant (MRSA) and the remaining two strains were methicillin-susceptible (MSSA). Twelve MRSA were mecC-positive, typed as t843-ST1583/ST1945/ST1581/ST1571 (n = 11) and t1535-ST1945 (n = 1) (all of clonal-complex CC130); they were susceptible to the non-β-lactams tested. The remaining MRSA strain carried the mecA gene, was typed as t011-ST398-CC398-agrI-SCCmec-V, and showed a multiresistance phenotype. MSSA isolates were ascribed to lineages ST97-CC97 and ST425-CC425. All S. aureus lacked the studied virulence genes (lukS/F-PV, tst, eta, etb, and etd), and the IEC type E (with scn and sak genes) was detected in four mecC-positive and one MSSA isolates. S. delphini strains were methicillin-susceptible but showed resistance to at least one of the antimicrobials tested, with high penicillin (75%, with blaZ gene) and tetracycline [58%, with tet(K)± tet(L)] resistance rates. All S. delphini isolates presented the virulence genes lukS-I, siet, and se-int, and four carried the clindamycin-resistance lnu(A) gene.

Authors+Show Affiliations

Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain.Grupo SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.SEO/BirdLife, Bird Monitoring Unit, Madrid, Spain. Parque Nacional de la Sierra de Guadarrama, Centro de Investigación, Seguimiento y Evaluación, Rascafría, Madrid, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain.Grupo SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Zoobotánico Jerez, Jerez de la Frontera, Spain.Aquila Foundation, Oropesa, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain.Grupo SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain. carmen.torres@unirioja.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30694341

Citation

Ruiz-Ripa, Laura, et al. "Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain." Microbial Ecology, vol. 78, no. 2, 2019, pp. 409-415.
Ruiz-Ripa L, Gómez P, Alonso CA, et al. Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain. Microb Ecol. 2019;78(2):409-415.
Ruiz-Ripa, L., Gómez, P., Alonso, C. A., Camacho, M. C., de la Puente, J., Fernández-Fernández, R., Ramiro, Y., Quevedo, M. A., Blanco, J. M., Zarazaga, M., Höfle, U., & Torres, C. (2019). Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain. Microbial Ecology, 78(2), 409-415. https://doi.org/10.1007/s00248-019-01328-4
Ruiz-Ripa L, et al. Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain. Microb Ecol. 2019;78(2):409-415. PubMed PMID: 30694341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain. AU - Ruiz-Ripa,Laura, AU - Gómez,Paula, AU - Alonso,Carla Andrea, AU - Camacho,Maria Cruz, AU - de la Puente,Javier, AU - Fernández-Fernández,Rosa, AU - Ramiro,Yolanda, AU - Quevedo,Miguel Angel, AU - Blanco,Juan Manuel, AU - Zarazaga,Myriam, AU - Höfle,Ursula, AU - Torres,Carmen, Y1 - 2019/01/29/ PY - 2018/08/22/received PY - 2019/01/15/accepted PY - 2019/1/30/pubmed PY - 2019/7/19/medline PY - 2019/1/30/entrez KW - CoPS KW - S. aureus KW - S. delphini KW - Wild birds KW - mecA-MRSA KW - mecC-MRSA SP - 409 EP - 415 JF - Microbial ecology JO - Microb Ecol VL - 78 IS - 2 N2 - The aim of this study was to determine the carriage rate of coagulase-positive staphylococci (CoPS) in wild birds and to characterize recovered isolates. Tracheal samples from 324 wild birds, obtained in different Spanish regions during 2015-2016, were screened for CoPS carriage. The antimicrobial resistance profile and the virulence gene content were investigated. Molecular typing was performed by spa, agr, MLST, SCCmec, and S. delphini group classification. CoPS were recovered from 26 samples of wild birds (8.3%), and 27 isolates were further characterized. Two CoPS species were detected: S. aureus (n = 15; eight cinereous vultures and seven magpies) and S. delphini (n = 12; 11 cinereous vultures and one red kite). Thirteen S. aureus were methicillin-resistant (MRSA) and the remaining two strains were methicillin-susceptible (MSSA). Twelve MRSA were mecC-positive, typed as t843-ST1583/ST1945/ST1581/ST1571 (n = 11) and t1535-ST1945 (n = 1) (all of clonal-complex CC130); they were susceptible to the non-β-lactams tested. The remaining MRSA strain carried the mecA gene, was typed as t011-ST398-CC398-agrI-SCCmec-V, and showed a multiresistance phenotype. MSSA isolates were ascribed to lineages ST97-CC97 and ST425-CC425. All S. aureus lacked the studied virulence genes (lukS/F-PV, tst, eta, etb, and etd), and the IEC type E (with scn and sak genes) was detected in four mecC-positive and one MSSA isolates. S. delphini strains were methicillin-susceptible but showed resistance to at least one of the antimicrobials tested, with high penicillin (75%, with blaZ gene) and tetracycline [58%, with tet(K)± tet(L)] resistance rates. All S. delphini isolates presented the virulence genes lukS-I, siet, and se-int, and four carried the clindamycin-resistance lnu(A) gene. SN - 1432-184X UR - https://www.unboundmedicine.com/medline/citation/30694341/Detection_of_MRSA_of_Lineages_CC130_mecC_and_CC398_mecA_and_Staphylococcus_delphini_lnu_A__in_Magpies_and_Cinereous_Vultures_in_Spain_ L2 - https://dx.doi.org/10.1007/s00248-019-01328-4 DB - PRIME DP - Unbound Medicine ER -