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[IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH].

Abstract

AIM

Determine an optimal set of the most effective methods of identification and intraspecies typing ofcausative agents ofglanders and melioidosis. Materials andmethods. Bacteriologic, immunochemical, molecular-genetic methods were used.

RESULTS

A possibility to identify collection strains of pathogenic and closely related Burkholderia in semiautomatic systems is studied. Means of detection of informative variable genome segments ofthe specified microorganisms were developed, methods of their genetic typing were selected. Effectiveness of application of precipitating mAbs for differentiation of Burkholderia was established. Data on diagnostic possibilities of immunoglobulins fluorescing based on monoclonal antibodies of various etiotropic directionality for detection and identification of B. mallei and B. pseudomallei are generalized. Experimental series of amplification test-systems for identification of glanders and melioidosis causative agents in real-time PCR format are created.

CONCLUSION

A number of methods for identification and typing of glanders and melioidosis causative agents is proposed.

Authors

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Pub Type(s)

Journal Article

Language

rus

PubMed ID

30695386

Citation

Korsakova, I I., et al. "[IDENTIFICATION of CAUSATIVE AGENTS of GLANDERS and MELIOIDOSIS BASED ON PRINCIPLES of POLYPHASE TAXONOMIC APPROACH]." Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii, 2016, pp. 25-34.
Korsakova II, Antonov VA, Khrapova NP, et al. [IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH]. Zh Mikrobiol Epidemiol Immunobiol. 2016.
Korsakova, I. I., Antonov, V. A., Khrapova, N. P., Zamarina, T. V., Pimenova, E. V., Kim, E. E., Merinova, L. K., Senina, T. V., Tkachenko, G. A., Savchenko, S. S., Ageeva, N. P., Molchanova, E. V., Lopasteiskaya, Y. A., & Prokhvatilova, E. V. (2016). [IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH]. Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii, (6), 25-34.
Korsakova II, et al. [IDENTIFICATION of CAUSATIVE AGENTS of GLANDERS and MELIOIDOSIS BASED ON PRINCIPLES of POLYPHASE TAXONOMIC APPROACH]. Zh Mikrobiol Epidemiol Immunobiol. 2016;(6)25-34. PubMed PMID: 30695386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH]. AU - Korsakova,I I, AU - Antonov,V A, AU - Khrapova,N P, AU - Zamarina,T V, AU - Pimenova,E V, AU - Kim,E E, AU - Merinova,L K, AU - Senina,T V, AU - Tkachenko,G A, AU - Savchenko,S S, AU - Ageeva,N P, AU - Molchanova,E V, AU - Lopasteiskaya,Ya A, AU - Prokhvatilova,E V, PY - 2019/1/30/entrez PY - 2016/11/1/pubmed PY - 2016/11/1/medline SP - 25 EP - 34 JF - Zhurnal mikrobiologii, epidemiologii i immunobiologii JO - Zh Mikrobiol Epidemiol Immunobiol IS - 6 N2 - AIM: Determine an optimal set of the most effective methods of identification and intraspecies typing ofcausative agents ofglanders and melioidosis. Materials andmethods. Bacteriologic, immunochemical, molecular-genetic methods were used. RESULTS: A possibility to identify collection strains of pathogenic and closely related Burkholderia in semiautomatic systems is studied. Means of detection of informative variable genome segments ofthe specified microorganisms were developed, methods of their genetic typing were selected. Effectiveness of application of precipitating mAbs for differentiation of Burkholderia was established. Data on diagnostic possibilities of immunoglobulins fluorescing based on monoclonal antibodies of various etiotropic directionality for detection and identification of B. mallei and B. pseudomallei are generalized. Experimental series of amplification test-systems for identification of glanders and melioidosis causative agents in real-time PCR format are created. CONCLUSION: A number of methods for identification and typing of glanders and melioidosis causative agents is proposed. SN - 0372-9311 UR - https://www.unboundmedicine.com/medline/citation/30695386/[IDENTIFICATION_OF_CAUSATIVE_AGENTS_OF_GLANDERS_AND_MELIOIDOSIS_BASED_ON_PRINCIPLES_OF_POLYPHASE_TAXONOMIC_APPROACH]_ L2 - http://www.diseaseinfosearch.org/result/3062 DB - PRIME DP - Unbound Medicine ER -