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The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n-6 and n-3 Polyunsaturated Fatty Acids in Male and Female Rats.
Lipids. 2019 01; 54(1):67-80.L

Abstract

Oxylipins are bioactive lipids formed by the monooxygenation of polyunsaturated fatty acids (PUFA). Eicosanoids derived from arachidonic acid (ARA) are the most well-studied class of oxylipins that influence brain functions in normal health and in disease. However, comprehensive profiling of brain oxylipins from other PUFA with differing functions, and the examination of the effects of dietary PUFA and sex differences in oxylipins are warranted. Therefore, female and male Sprague-Dawley rats were provided standard rodent diets that provided additional levels of the individual n-3 PUFA α-linolenic acid (ALA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), or the n-6 PUFA linoleic acid (LNA) alone or with ALA (LNA + ALA) compared to essential fatty acid-sufficient control diets. Oxylipins and PUFA were quantified in whole brains using HPLC-MS/MS and GC, respectively. Eighty-seven oxylipins were present at quantifiable levels: 51% and 17% of these were derived from ARA and DHA, respectively. At the mass level, ARA and DHA oxylipins comprised 81-90% and 6-12% of total oxylipins, while phospholipid ARA and DHA represented 25-35% and 49-62% of PUFA mass, respectively. Increasing dietary n-3 PUFA resulted in higher levels of oxylipins derived from their precursor PUFA; otherwise, the brain oxylipin profile was largely resistant to modulation by diet. Approximately 25% of oxylipins were higher in males, and this was largely unaffected by diet, further revealing a tight regulation of brain oxylipin levels. These fundamental data on brain oxylipin composition, diet effects, and sex differences will help guide future studies examining the functions of oxylipins in the brain.

Authors+Show Affiliations

Department of Food and Human Nutritional Sciences, 190 Dysart Road, University of Manitoba Winnipeg, Canada R3T 2N2. Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Ave, Winnipeg, Canada R2H 2A6.Department of Food and Human Nutritional Sciences, 190 Dysart Road, University of Manitoba Winnipeg, Canada R3T 2N2. Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Ave, Winnipeg, Canada R2H 2A6.Department of Food and Human Nutritional Sciences, 190 Dysart Road, University of Manitoba Winnipeg, Canada R3T 2N2. Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Ave, Winnipeg, Canada R2H 2A6.Department of Food and Human Nutritional Sciences, 190 Dysart Road, University of Manitoba Winnipeg, Canada R3T 2N2. Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Ave, Winnipeg, Canada R2H 2A6.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30697757

Citation

Ferdouse, Afroza, et al. "The Brain Oxylipin Profile Is Resistant to Modulation By Dietary N-6 and N-3 Polyunsaturated Fatty Acids in Male and Female Rats." Lipids, vol. 54, no. 1, 2019, pp. 67-80.
Ferdouse A, Leng S, Winter T, et al. The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n-6 and n-3 Polyunsaturated Fatty Acids in Male and Female Rats. Lipids. 2019;54(1):67-80.
Ferdouse, A., Leng, S., Winter, T., & Aukema, H. M. (2019). The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n-6 and n-3 Polyunsaturated Fatty Acids in Male and Female Rats. Lipids, 54(1), 67-80. https://doi.org/10.1002/lipd.12122
Ferdouse A, et al. The Brain Oxylipin Profile Is Resistant to Modulation By Dietary N-6 and N-3 Polyunsaturated Fatty Acids in Male and Female Rats. Lipids. 2019;54(1):67-80. PubMed PMID: 30697757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Brain Oxylipin Profile Is Resistant to Modulation by Dietary n-6 and n-3 Polyunsaturated Fatty Acids in Male and Female Rats. AU - Ferdouse,Afroza, AU - Leng,Shan, AU - Winter,Tanja, AU - Aukema,Harold M, Y1 - 2019/01/29/ PY - 2018/10/30/received PY - 2018/12/17/revised PY - 2018/12/18/accepted PY - 2019/1/31/pubmed PY - 2019/9/3/medline PY - 2019/1/31/entrez KW - Brain KW - Dietary n-3 PUFA KW - Dietary n-6 PUFA KW - Eicosanoids KW - Oxylipins KW - Sex SP - 67 EP - 80 JF - Lipids JO - Lipids VL - 54 IS - 1 N2 - Oxylipins are bioactive lipids formed by the monooxygenation of polyunsaturated fatty acids (PUFA). Eicosanoids derived from arachidonic acid (ARA) are the most well-studied class of oxylipins that influence brain functions in normal health and in disease. However, comprehensive profiling of brain oxylipins from other PUFA with differing functions, and the examination of the effects of dietary PUFA and sex differences in oxylipins are warranted. Therefore, female and male Sprague-Dawley rats were provided standard rodent diets that provided additional levels of the individual n-3 PUFA α-linolenic acid (ALA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), or the n-6 PUFA linoleic acid (LNA) alone or with ALA (LNA + ALA) compared to essential fatty acid-sufficient control diets. Oxylipins and PUFA were quantified in whole brains using HPLC-MS/MS and GC, respectively. Eighty-seven oxylipins were present at quantifiable levels: 51% and 17% of these were derived from ARA and DHA, respectively. At the mass level, ARA and DHA oxylipins comprised 81-90% and 6-12% of total oxylipins, while phospholipid ARA and DHA represented 25-35% and 49-62% of PUFA mass, respectively. Increasing dietary n-3 PUFA resulted in higher levels of oxylipins derived from their precursor PUFA; otherwise, the brain oxylipin profile was largely resistant to modulation by diet. Approximately 25% of oxylipins were higher in males, and this was largely unaffected by diet, further revealing a tight regulation of brain oxylipin levels. These fundamental data on brain oxylipin composition, diet effects, and sex differences will help guide future studies examining the functions of oxylipins in the brain. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/30697757/The_Brain_Oxylipin_Profile_Is_Resistant_to_Modulation_by_Dietary_n_6_and_n_3_Polyunsaturated_Fatty_Acids_in_Male_and_Female_Rats_ L2 - https://doi.org/10.1002/lipd.12122 DB - PRIME DP - Unbound Medicine ER -