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Scaffolding adapter protein Gab1 impairs bFGF-induced bio-activity via affecting PI3K-AKT pathway.
J Biol Regul Homeost Agents 2019 Jan-Feb,; 33(1):53-62JB

Abstract

The role of Grb2-associated binder 1 (Gab1) in bFGF-activated PI3K-AKT pathway of endothelial cells remains largely unknown. To elucidate this role, a set of studies with siRNA knockdown of Gab1 was performed. Knockdown of Gab1 using siRNA was performed in fused endothelial cell line EA.hy926 and the low level of Gab1 was confirmed with quantitative R-T PCR and Western blotting. Effects of Gab1 down-regulation were examined on several aspects: bFGF-induced AKT phosphorylation, proliferation, migration and vessel tubing formation of EA.hy926 cells. The bFGF-induced AKT phosphorylation of wild-type EA.hy926 cells was both dose-dependent and time dependent with a peak at 10 ng/ml and about 30 min after bFGF treatment. The AKT activation was significantly reduced in Gab1 siRNA-treated EA.hy926 cells. The blocking of Gab1-AKT path resulted in a set of biological alterations of EA.hy926 cells: (i) reduced proliferation; (ii) impaired migration; (iii) decreased vessel tubing formation in both 2D and 3D culture. All data support that Gab1 is associated with angiogenesis function of EA.hy926 endothelium cells via PI3K-Akt signaling pathway.

Authors+Show Affiliations

Department of Vascular Surgery, the First Affiliated Hospital of Fujian Medical University, Abdominal Surgery Institute of Fujian Province, Fuzhou, China.Department of Vascular Surgery, the First Affiliated Hospital of Fujian Medical University, Abdominal Surgery Institute of Fujian Province, Fuzhou, China.Department of Vascular Surgery, the First Affiliated Hospital of Fujian Medical University, Abdominal Surgery Institute of Fujian Province, Fuzhou, China.Department of Vascular Surgery, the First Affiliated Hospital of Fujian Medical University, Abdominal Surgery Institute of Fujian Province, Fuzhou, China.Fujian Key Laboratory of Individualized Active Immunotherapy and Key Laboratory of Radiation Biology, Fujian Universities, Fuzhou, China. Department of Radiation Oncology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, China. Department of Radiation Oncology, University of Florida, Gainesville, Florida, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30697991

Citation

Zhang, J C., et al. "Scaffolding Adapter Protein Gab1 Impairs bFGF-induced Bio-activity Via Affecting PI3K-AKT Pathway." Journal of Biological Regulators and Homeostatic Agents, vol. 33, no. 1, 2019, pp. 53-62.
Zhang JC, Li WX, Wu L, et al. Scaffolding adapter protein Gab1 impairs bFGF-induced bio-activity via affecting PI3K-AKT pathway. J Biol Regul Homeost Agents. 2019;33(1):53-62.
Zhang, J. C., Li, W. X., Wu, L., Liu, X., & Zhang, L. (2019). Scaffolding adapter protein Gab1 impairs bFGF-induced bio-activity via affecting PI3K-AKT pathway. Journal of Biological Regulators and Homeostatic Agents, 33(1), pp. 53-62.
Zhang JC, et al. Scaffolding Adapter Protein Gab1 Impairs bFGF-induced Bio-activity Via Affecting PI3K-AKT Pathway. J Biol Regul Homeost Agents. 2019;33(1):53-62. PubMed PMID: 30697991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Scaffolding adapter protein Gab1 impairs bFGF-induced bio-activity via affecting PI3K-AKT pathway. AU - Zhang,J C, AU - Li,W X, AU - Wu,L, AU - Liu,X, AU - Zhang,L, PY - 2019/1/31/entrez PY - 2019/1/31/pubmed PY - 2019/6/18/medline SP - 53 EP - 62 JF - Journal of biological regulators and homeostatic agents JO - J. Biol. Regul. Homeost. Agents VL - 33 IS - 1 N2 - The role of Grb2-associated binder 1 (Gab1) in bFGF-activated PI3K-AKT pathway of endothelial cells remains largely unknown. To elucidate this role, a set of studies with siRNA knockdown of Gab1 was performed. Knockdown of Gab1 using siRNA was performed in fused endothelial cell line EA.hy926 and the low level of Gab1 was confirmed with quantitative R-T PCR and Western blotting. Effects of Gab1 down-regulation were examined on several aspects: bFGF-induced AKT phosphorylation, proliferation, migration and vessel tubing formation of EA.hy926 cells. The bFGF-induced AKT phosphorylation of wild-type EA.hy926 cells was both dose-dependent and time dependent with a peak at 10 ng/ml and about 30 min after bFGF treatment. The AKT activation was significantly reduced in Gab1 siRNA-treated EA.hy926 cells. The blocking of Gab1-AKT path resulted in a set of biological alterations of EA.hy926 cells: (i) reduced proliferation; (ii) impaired migration; (iii) decreased vessel tubing formation in both 2D and 3D culture. All data support that Gab1 is associated with angiogenesis function of EA.hy926 endothelium cells via PI3K-Akt signaling pathway. SN - 0393-974X UR - https://www.unboundmedicine.com/medline/citation/30697991/Scaffolding_adapter_protein_Gab1_impairs_bFGF-induced_bio-activity_via_affecting_PI3K-AKT_pathway DB - PRIME DP - Unbound Medicine ER -