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Amplification of different ColE1 plasmids in an Escherichia coli relA strain.
J Basic Microbiol. 1988; 28(8):553-5.JB

Abstract

Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColE1 related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation in order to determine the upper plasmid level in amino acid starved cells. In all cases we measured a 4 to 6 fold increase of the plasmid copy number in comparison to log-phase cells independent of the size, the number of origins per plasmid molecule or the copy number in log-phase cells. The plasmid copy number in amino acid starved cells varies from about 200 (pBR322-dimer) to about 2000 (high copy number plasmid pERIII-BPL4, see Boros et al. 1986). Rop+ and rop- plasmids show the same amplification rate under the used conditions.

Authors+Show Affiliations

Sektion Biologie, WB Allgemeine Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3070008

Citation

Schroeter, A, et al. "Amplification of Different ColE1 Plasmids in an Escherichia Coli relA Strain." Journal of Basic Microbiology, vol. 28, no. 8, 1988, pp. 553-5.
Schroeter A, Riethdorf S, Hecker M. Amplification of different ColE1 plasmids in an Escherichia coli relA strain. J Basic Microbiol. 1988;28(8):553-5.
Schroeter, A., Riethdorf, S., & Hecker, M. (1988). Amplification of different ColE1 plasmids in an Escherichia coli relA strain. Journal of Basic Microbiology, 28(8), 553-5.
Schroeter A, Riethdorf S, Hecker M. Amplification of Different ColE1 Plasmids in an Escherichia Coli relA Strain. J Basic Microbiol. 1988;28(8):553-5. PubMed PMID: 3070008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amplification of different ColE1 plasmids in an Escherichia coli relA strain. AU - Schroeter,A, AU - Riethdorf,S, AU - Hecker,M, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 553 EP - 5 JF - Journal of basic microbiology JO - J. Basic Microbiol. VL - 28 IS - 8 N2 - Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColE1 related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation in order to determine the upper plasmid level in amino acid starved cells. In all cases we measured a 4 to 6 fold increase of the plasmid copy number in comparison to log-phase cells independent of the size, the number of origins per plasmid molecule or the copy number in log-phase cells. The plasmid copy number in amino acid starved cells varies from about 200 (pBR322-dimer) to about 2000 (high copy number plasmid pERIII-BPL4, see Boros et al. 1986). Rop+ and rop- plasmids show the same amplification rate under the used conditions. SN - 0233-111X UR - https://www.unboundmedicine.com/medline/citation/3070008/Amplification_of_different_ColE1_plasmids_in_an_Escherichia_coli_relA_strain_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0233-111X&date=1988&volume=28&issue=8&spage=553 DB - PRIME DP - Unbound Medicine ER -