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Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan.
Trop Anim Health Prod. 2019 Jun; 51(5):1259-1265.TA

Abstract

Avian pathogenic Escherichia coli (APEC) causes colibacillosis that leads to high morbidity and mortality among poultry birds. To date, there is a lack of knowledge about virulence-associated genes (VAGs) and multidrug resistance of APEC isolates from Pakistan. In this study, we determined the VAGs and antibiotic resistance profiles of APEC isolates recovered from colibacillosis affected broilers in Faisalabad region of Pakistan. A total of 84 diseased and dead birds from different local broilers farms were collected and examined for the gross lesions of colibacillosis by conducting postmortem examination. Of these, APEC isolates were recovered from 75 (89.2%) birds. Antibiotic susceptibility tests against 11 antimicrobial agents showed the highest resistance against ampicillin (98.6%) followed by tetracycline (97.3%) and ciprofloxacin (72%). The presence of 11 virulence-associated genes (VAGs) was detected by multiplex polymerase chain reaction (PCR). Of the 75 APEC, 32 (42.6%) harbored > 5 VAGs. Most commonly found genes were increased serum survival (iss; 84%), iron transport (iutA; 74.6%), and colicin V (ColV; 60%). Twenty-two isolates (29.3%) were found to possess a combination of VAGs; iss, tsh, iroN, and iutA, in addition to other VAGs. To the best of our knowledge, this is the first report on the detection of virulence-associated genes and multidrug resistance among APEC isolates in Pakistan. In the future, the strains with the predominant set of VAGs can be used for colibacillosis diagnosis and as a potential vaccine candidate.

Authors+Show Affiliations

Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan.Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan. mashkoormohsin@uaf.edu.pk.Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan.Department of Pathology, University of Agriculture, Faisalabad, 38040, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30701453

Citation

Azam, Mariya, et al. "Virulence-associated Genes and Antimicrobial Resistance Among Avian Pathogenic Escherichia Coli From Colibacillosis Affected Broilers in Pakistan." Tropical Animal Health and Production, vol. 51, no. 5, 2019, pp. 1259-1265.
Azam M, Mohsin M, Sajjad-Ur-Rahman , et al. Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan. Trop Anim Health Prod. 2019;51(5):1259-1265.
Azam, M., Mohsin, M., Sajjad-Ur-Rahman, ., & Saleemi, M. K. (2019). Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan. Tropical Animal Health and Production, 51(5), 1259-1265. https://doi.org/10.1007/s11250-019-01823-3
Azam M, et al. Virulence-associated Genes and Antimicrobial Resistance Among Avian Pathogenic Escherichia Coli From Colibacillosis Affected Broilers in Pakistan. Trop Anim Health Prod. 2019;51(5):1259-1265. PubMed PMID: 30701453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan. AU - Azam,Mariya, AU - Mohsin,Mashkoor, AU - Sajjad-Ur-Rahman,, AU - Saleemi,Muhammad Kashif, Y1 - 2019/01/30/ PY - 2018/11/08/received PY - 2019/01/21/accepted PY - 2019/2/1/pubmed PY - 2019/7/30/medline PY - 2019/2/1/entrez KW - Antimicrobial resistance KW - Avian pathogenic E. coli KW - Colibacillosis KW - Poultry KW - Virulence genes SP - 1259 EP - 1265 JF - Tropical animal health and production JO - Trop Anim Health Prod VL - 51 IS - 5 N2 - Avian pathogenic Escherichia coli (APEC) causes colibacillosis that leads to high morbidity and mortality among poultry birds. To date, there is a lack of knowledge about virulence-associated genes (VAGs) and multidrug resistance of APEC isolates from Pakistan. In this study, we determined the VAGs and antibiotic resistance profiles of APEC isolates recovered from colibacillosis affected broilers in Faisalabad region of Pakistan. A total of 84 diseased and dead birds from different local broilers farms were collected and examined for the gross lesions of colibacillosis by conducting postmortem examination. Of these, APEC isolates were recovered from 75 (89.2%) birds. Antibiotic susceptibility tests against 11 antimicrobial agents showed the highest resistance against ampicillin (98.6%) followed by tetracycline (97.3%) and ciprofloxacin (72%). The presence of 11 virulence-associated genes (VAGs) was detected by multiplex polymerase chain reaction (PCR). Of the 75 APEC, 32 (42.6%) harbored > 5 VAGs. Most commonly found genes were increased serum survival (iss; 84%), iron transport (iutA; 74.6%), and colicin V (ColV; 60%). Twenty-two isolates (29.3%) were found to possess a combination of VAGs; iss, tsh, iroN, and iutA, in addition to other VAGs. To the best of our knowledge, this is the first report on the detection of virulence-associated genes and multidrug resistance among APEC isolates in Pakistan. In the future, the strains with the predominant set of VAGs can be used for colibacillosis diagnosis and as a potential vaccine candidate. SN - 1573-7438 UR - https://www.unboundmedicine.com/medline/citation/30701453/Virulence_associated_genes_and_antimicrobial_resistance_among_avian_pathogenic_Escherichia_coli_from_colibacillosis_affected_broilers_in_Pakistan_ L2 - https://dx.doi.org/10.1007/s11250-019-01823-3 DB - PRIME DP - Unbound Medicine ER -