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Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity.
Int J Nanomedicine. 2019; 14:667-687.IJ

Abstract

Purpose

The biomedical applications of silver nanoparticles (AgNPs) are heavily investigated due to their cytotoxic and antimicrobial properties. However, the scientific literature is lacking in data on the aggregation behavior of nanoparticles, especially regarding its impact on biological activity. Therefore, to assess the potential of AgNPs in therapeutic applications, two different AgNP samples were compared under biorelevant conditions.

Methods

Citrate-capped nanosilver was produced by classical chemical reduction and stabilization with sodium citrate (AgNP@C), while green tea extract was used to produce silver nanoparticles in a green synthesis approach (AgNP@GTs). Particle size, morphology, and crystallinity were characterized using transmission electron microscopy. To observe the effects of the most important biorelevant conditions on AgNP colloidal stability, aggregation grade measurements were carried out using UV-Vis spectroscopy and dynamic light scatterig, while MTT assay and a microdilution method were performed to evaluate the effects of aggregation on cytotoxicity and antimicrobial activity in a time-dependent manner.

Results

The aggregation behavior of AgNPs is mostly affected by pH and electrolyte concentration, while the presence of biomolecules can improve particle stability due to the biomolecular corona effect. We demonstrated that high aggregation grade in both AgNP samples attenuated their toxic effect toward living cells. However, AgNP@GT proved less prone to aggregation thus retained a degree of its toxicity.

Conclusion

To our knowledge, this is the first systematic examination regarding AgNP aggregation behavior with simultaneous measurements of its effect on biological activity. We showed that nanoparticle behavior in complex systems can be estimated by simple compounds like sodium chloride and glutamine. Electrostatic stabilization might not be suitable for biomedical AgNP applications, while green synthesis approaches could offer new frontiers to preserve nanoparticle toxicity by enhancing colloidal stability. The importance of properly selected synthesis methods must be emphasized as they profoundly influence colloidal stability, and therefore biological activity.

Authors+Show Affiliations

Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary, konya@chem.u-szeged.hu.Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary, konya@chem.u-szeged.hu. Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary, konya@chem.u-szeged.hu.Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary, konya@chem.u-szeged.hu. MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Szeged, Hungary, konya@chem.u-szeged.hu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30705586

Citation

Bélteky, Péter, et al. "Silver Nanoparticles: Aggregation Behavior in Biorelevant Conditions and Its Impact On Biological Activity." International Journal of Nanomedicine, vol. 14, 2019, pp. 667-687.
Bélteky P, Rónavári A, Igaz N, et al. Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity. Int J Nanomedicine. 2019;14:667-687.
Bélteky, P., Rónavári, A., Igaz, N., Szerencsés, B., Tóth, I. Y., Pfeiffer, I., Kiricsi, M., & Kónya, Z. (2019). Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity. International Journal of Nanomedicine, 14, 667-687. https://doi.org/10.2147/IJN.S185965
Bélteky P, et al. Silver Nanoparticles: Aggregation Behavior in Biorelevant Conditions and Its Impact On Biological Activity. Int J Nanomedicine. 2019;14:667-687. PubMed PMID: 30705586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity. AU - Bélteky,Péter, AU - Rónavári,Andrea, AU - Igaz,Nóra, AU - Szerencsés,Bettina, AU - Tóth,Ildikó Y, AU - Pfeiffer,Ilona, AU - Kiricsi,Mónika, AU - Kónya,Zoltán, Y1 - 2019/01/18/ PY - 2019/2/2/entrez PY - 2019/2/2/pubmed PY - 2019/3/21/medline KW - antimicrobial activity KW - colloidal stability KW - cytotoxicity KW - green synthesis SP - 667 EP - 687 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 14 N2 - Purpose: The biomedical applications of silver nanoparticles (AgNPs) are heavily investigated due to their cytotoxic and antimicrobial properties. However, the scientific literature is lacking in data on the aggregation behavior of nanoparticles, especially regarding its impact on biological activity. Therefore, to assess the potential of AgNPs in therapeutic applications, two different AgNP samples were compared under biorelevant conditions. Methods: Citrate-capped nanosilver was produced by classical chemical reduction and stabilization with sodium citrate (AgNP@C), while green tea extract was used to produce silver nanoparticles in a green synthesis approach (AgNP@GTs). Particle size, morphology, and crystallinity were characterized using transmission electron microscopy. To observe the effects of the most important biorelevant conditions on AgNP colloidal stability, aggregation grade measurements were carried out using UV-Vis spectroscopy and dynamic light scatterig, while MTT assay and a microdilution method were performed to evaluate the effects of aggregation on cytotoxicity and antimicrobial activity in a time-dependent manner. Results: The aggregation behavior of AgNPs is mostly affected by pH and electrolyte concentration, while the presence of biomolecules can improve particle stability due to the biomolecular corona effect. We demonstrated that high aggregation grade in both AgNP samples attenuated their toxic effect toward living cells. However, AgNP@GT proved less prone to aggregation thus retained a degree of its toxicity. Conclusion: To our knowledge, this is the first systematic examination regarding AgNP aggregation behavior with simultaneous measurements of its effect on biological activity. We showed that nanoparticle behavior in complex systems can be estimated by simple compounds like sodium chloride and glutamine. Electrostatic stabilization might not be suitable for biomedical AgNP applications, while green synthesis approaches could offer new frontiers to preserve nanoparticle toxicity by enhancing colloidal stability. The importance of properly selected synthesis methods must be emphasized as they profoundly influence colloidal stability, and therefore biological activity. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/30705586/Silver_nanoparticles:_aggregation_behavior_in_biorelevant_conditions_and_its_impact_on_biological_activity_ L2 - https://dx.doi.org/10.2147/IJN.S185965 DB - PRIME DP - Unbound Medicine ER -