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Structure and immunoregulatory activity of β-d-galactofuranose-containing polysaccharides from the medicinal fungus Shiraia bambusicola.
Int J Biol Macromol. 2019 May 15; 129:530-537.IJ

Abstract

To explore bioactive polysaccharides from medicinal fungi, a homogeneous heteropolysaccharide SB1-1 was extracted from the stroma of Shiraia bambusicola by enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatographies. Chemical and spectroscopic analyses showed that SB1-1 was a neutral polysaccharide composed of mannose, glucose and galactose at a molar ratio of 1.1:1.9:1.0, and had a molecular weight of 18.3 kDa. SB1-1 was composed of a mannan core and glucose and galactose side chains. The mannan core was composed of (1 → 2)-α-Manp substituted by the side chains (1 → 6)-α-d-Manp, (1 → 4)-α-d-Glcp and (1 → 6)-α-d-Glcp with different degrees of polymerization at the C-6. The galactose side chains had the backbone of (1 → 6)-β-d-Galf. There were two branch sites on every five sugars on average at the O-2 of →6)-β-Galf(1→, and the branches consisted of →2)-β-d-Galf(1→ and terminal β-d-Galf(1→. The potential immunomodulatory activities of SB1-1 on RAW264.7 macrophages were investigated. The results showed that SB1-1 could activate macrophage and significantly improve its phagocytic ability by neutral red uptake assay. Additionally, SB1-1 enhanced the productions of IL-1, IL-6, IL-12 and TNF-α in the RAW264.7 macrophages by stimulation of SB1-1, while significantly increased the production of inducible nitric oxide synthase (iNOS) production. This study demonstrated that the galactofuranose-containing polysaccharide SB1-1 from S. bambusicola which is a novel mannogalactoglucan, could enhance the activation of macrophages.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhou D
College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Li P
Marine School, Ningbo University, 818 Fenghua Road, Ningbo 315000, People's Republic of China; Zhejiang Mariculture Research Institute, 28 Tiyu Road, Zhoushan 316000, People's Republic of China.
Dong Z
College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Wang T
College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Sun K
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Zhao Y
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Wang B
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Chen Y
College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, People's Republic of China; Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, 1 South Haida Road, Zhoushan 316000, People's Republic of China. Electronic address: mojojo1984@163.com.

MeSH

AnimalsAscomycotaCarbohydrate SequenceFungal PolysaccharidesGalactoseHydrogen-Ion ConcentrationHydrolysisImmunologic FactorsMacrophagesMethylationMiceMonosaccharidesRAW 264.7 Cells

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30710588

Citation

Zhou, Dejian, et al. "Structure and Immunoregulatory Activity of Β-d-galactofuranose-containing Polysaccharides From the Medicinal Fungus Shiraia Bambusicola." International Journal of Biological Macromolecules, vol. 129, 2019, pp. 530-537.
Zhou D, Li P, Dong Z, et al. Structure and immunoregulatory activity of β-d-galactofuranose-containing polysaccharides from the medicinal fungus Shiraia bambusicola. Int J Biol Macromol. 2019;129:530-537.
Zhou, D., Li, P., Dong, Z., Wang, T., Sun, K., Zhao, Y., Wang, B., & Chen, Y. (2019). Structure and immunoregulatory activity of β-d-galactofuranose-containing polysaccharides from the medicinal fungus Shiraia bambusicola. International Journal of Biological Macromolecules, 129, 530-537. https://doi.org/10.1016/j.ijbiomac.2019.01.179
Zhou D, et al. Structure and Immunoregulatory Activity of Β-d-galactofuranose-containing Polysaccharides From the Medicinal Fungus Shiraia Bambusicola. Int J Biol Macromol. 2019 May 15;129:530-537. PubMed PMID: 30710588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure and immunoregulatory activity of β-d-galactofuranose-containing polysaccharides from the medicinal fungus Shiraia bambusicola. AU - Zhou,Dejian, AU - Li,Peipei, AU - Dong,Zhe, AU - Wang,Teng, AU - Sun,Kunlai, AU - Zhao,Yuqin, AU - Wang,Bin, AU - Chen,Yin, Y1 - 2019/01/30/ PY - 2018/11/26/received PY - 2019/01/03/revised PY - 2019/01/28/accepted PY - 2019/2/3/pubmed PY - 2019/7/13/medline PY - 2019/2/3/entrez KW - Galactofuranose KW - Macrophages KW - Medical fungi KW - Polysaccharide KW - Structure SP - 530 EP - 537 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 129 N2 - To explore bioactive polysaccharides from medicinal fungi, a homogeneous heteropolysaccharide SB1-1 was extracted from the stroma of Shiraia bambusicola by enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatographies. Chemical and spectroscopic analyses showed that SB1-1 was a neutral polysaccharide composed of mannose, glucose and galactose at a molar ratio of 1.1:1.9:1.0, and had a molecular weight of 18.3 kDa. SB1-1 was composed of a mannan core and glucose and galactose side chains. The mannan core was composed of (1 → 2)-α-Manp substituted by the side chains (1 → 6)-α-d-Manp, (1 → 4)-α-d-Glcp and (1 → 6)-α-d-Glcp with different degrees of polymerization at the C-6. The galactose side chains had the backbone of (1 → 6)-β-d-Galf. There were two branch sites on every five sugars on average at the O-2 of →6)-β-Galf(1→, and the branches consisted of →2)-β-d-Galf(1→ and terminal β-d-Galf(1→. The potential immunomodulatory activities of SB1-1 on RAW264.7 macrophages were investigated. The results showed that SB1-1 could activate macrophage and significantly improve its phagocytic ability by neutral red uptake assay. Additionally, SB1-1 enhanced the productions of IL-1, IL-6, IL-12 and TNF-α in the RAW264.7 macrophages by stimulation of SB1-1, while significantly increased the production of inducible nitric oxide synthase (iNOS) production. This study demonstrated that the galactofuranose-containing polysaccharide SB1-1 from S. bambusicola which is a novel mannogalactoglucan, could enhance the activation of macrophages. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30710588/Structure_and_immunoregulatory_activity_of_β_d_galactofuranose_containing_polysaccharides_from_the_medicinal_fungus_Shiraia_bambusicola_ DB - PRIME DP - Unbound Medicine ER -