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Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt.
Food Chem 2019; 282:48-57FC

Abstract

High cost and enzyme deactivation in purification process are the two main obstacles for the use of enzyme as green catalyst. The objective of this work was to overcome these limitations by developing a cost-effective aqueous two-phase system (ATPS) for efficient purification of enzymes with remarkable separation efficiency and high retention of enzyme activity. The ATPS was formed by thermo-responsive block copolymer PEG113-b-PNIPAM149 and salt as phase-forming components combining economy, recovery and sustainability. This strategy fabricated block copolymer with specified molecular weight and low LCST, which not only achieved better phase splitting but also ensured easy recycling for block copolymer. The developed ATPS demonstrated excellent extraction and biocompatibility for bromelain in real sample with 94.87% separation efficiency and 77.06% activity, which were remarkably higher than those obtained in EOPOEO-based ATPS. The recycling of copolymer was introduced to minimize cost, with recovery efficiency of 90% in the five cycles.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: yunwang@ujs.edu.cn.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30711105

Citation

Wang, Lei, et al. "Green Separation of Bromelain in Food Sample With High Retention of Enzyme Activity Using Recyclable Aqueous Two-phase System Containing a New Synthesized Thermo-responsive Copolymer and Salt." Food Chemistry, vol. 282, 2019, pp. 48-57.
Wang L, Li W, Liu Y, et al. Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt. Food Chem. 2019;282:48-57.
Wang, L., Li, W., Liu, Y., Zhi, W., Han, J., Wang, Y., & Ni, L. (2019). Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt. Food Chemistry, 282, pp. 48-57. doi:10.1016/j.foodchem.2019.01.005.
Wang L, et al. Green Separation of Bromelain in Food Sample With High Retention of Enzyme Activity Using Recyclable Aqueous Two-phase System Containing a New Synthesized Thermo-responsive Copolymer and Salt. Food Chem. 2019 Jun 1;282:48-57. PubMed PMID: 30711105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt. AU - Wang,Lei, AU - Li,Wenxuan, AU - Liu,Yuanyuan, AU - Zhi,Wenjing, AU - Han,Juan, AU - Wang,Yun, AU - Ni,Liang, Y1 - 2019/01/06/ PY - 2018/07/21/received PY - 2018/11/17/revised PY - 2019/01/03/accepted PY - 2019/2/4/entrez PY - 2019/2/4/pubmed PY - 2019/3/6/medline KW - Aqueous two-phase system KW - Bromelain KW - Reusability KW - Thermo-responsive block copolymer SP - 48 EP - 57 JF - Food chemistry JO - Food Chem VL - 282 N2 - High cost and enzyme deactivation in purification process are the two main obstacles for the use of enzyme as green catalyst. The objective of this work was to overcome these limitations by developing a cost-effective aqueous two-phase system (ATPS) for efficient purification of enzymes with remarkable separation efficiency and high retention of enzyme activity. The ATPS was formed by thermo-responsive block copolymer PEG113-b-PNIPAM149 and salt as phase-forming components combining economy, recovery and sustainability. This strategy fabricated block copolymer with specified molecular weight and low LCST, which not only achieved better phase splitting but also ensured easy recycling for block copolymer. The developed ATPS demonstrated excellent extraction and biocompatibility for bromelain in real sample with 94.87% separation efficiency and 77.06% activity, which were remarkably higher than those obtained in EOPOEO-based ATPS. The recycling of copolymer was introduced to minimize cost, with recovery efficiency of 90% in the five cycles. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/30711105/Green_separation_of_bromelain_in_food_sample_with_high_retention_of_enzyme_activity_using_recyclable_aqueous_two-phase_system_containing_a_new_synthesized_thermo-responsive_copolymer_and_salt L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(19)30026-3 DB - PRIME DP - Unbound Medicine ER -