Tags

Type your tag names separated by a space and hit enter

Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate induced oxidative stress and DNA damage in Clarias gariepinus.
Environ Pollut. 2019 Apr; 247:678-684.EP

Abstract

Pyrazole derivatives display diverse biological and pharmacological activities. The aim of this study is to investigate the antioxidant properties of a novel pyrazolecarboxamide derivative (4-amino-N-[(4-chlorophenyl)]-3-methyl-1-phenyl-1H-thieno [2, 3-c] pyrazole-5-carboxamide) in African catfish, Clarias gariepinus, exposed to 1 mg/L PbNO3. Fish were intramuscularly injected with pyrazole-5-carboxamidederivative according to the following groupings: Group 1 (control), Group 2 (1 mg/L lead nitrate), Group 3 (1 mg/L lead nitrate + 5 mg pyrazole derivative/kg body weight), and Group 4 (1 mg/L lead nitrate + 10 mg pyrazole derivative/kg body weight) for two weeks and four weeks. Lead nitrate (1 mg/L) caused significant elevation of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, uric acid, cholesterol, and glucose-6-phosphate dehydrogenase (G6PDH) compared to the control group after two and four weeks of exposure, while serum total lipids, alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were significantly reduced compared to the control group. Furthermore, levels of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and total antioxidant capacity (TAC) were reduced in group 2 compared to the control group. However, in group 2, hepatic lipid peroxidation (LPO) and DNA fragmentation percentage were significantly increased compared to the control group. Histopathological changes in the liver of lead-exposed groups included marked disturbance of hepatic tissue organization, degeneration of hepatocytes, dilation of blood sinusoids and the central vein as well as necrosis. Injection of pyrazole derivative for two weeks and four weeks reversed alterations in biochemical parameters, antioxidant biomarkers, lipid peroxidation, hepatic DNA damage, and histopathological changes in liver tissue induced by 1 mg/L lead nitrate. This amelioration was higher in response to high-dose pyrazole derivative (10 mg) at the fourth week of exposure, showing concentration-and time-dependency. Overall, the sensitized derivative pyrazolecarboxamide is likely a useful tool to minimize the effects of lead toxicity due to its potent antioxidant activity.

Authors+Show Affiliations

Department of Zoology, Faculty of Science, Sohag University, 8562, Sohag, Egypt.Department of Zoology, Faculty of Science, Al Azhar University (Assiut Branch), 71524, Assiut, Egypt.Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, 16419, South Korea.Department of Zoology, Faculty of Science, Assiut University, 71516, Assiut, Egypt. Electronic address: alaasayed@aun.edu.eg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30711823

Citation

Soliman, Hamdy A M., et al. "Protective Effects of a Novel Pyrazolecarboxamide Derivative Against Lead Nitrate Induced Oxidative Stress and DNA Damage in Clarias Gariepinus." Environmental Pollution (Barking, Essex : 1987), vol. 247, 2019, pp. 678-684.
Soliman HAM, Hamed M, Lee JS, et al. Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate induced oxidative stress and DNA damage in Clarias gariepinus. Environ Pollut. 2019;247:678-684.
Soliman, H. A. M., Hamed, M., Lee, J. S., & Sayed, A. E. H. (2019). Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate induced oxidative stress and DNA damage in Clarias gariepinus. Environmental Pollution (Barking, Essex : 1987), 247, 678-684. https://doi.org/10.1016/j.envpol.2019.01.074
Soliman HAM, et al. Protective Effects of a Novel Pyrazolecarboxamide Derivative Against Lead Nitrate Induced Oxidative Stress and DNA Damage in Clarias Gariepinus. Environ Pollut. 2019;247:678-684. PubMed PMID: 30711823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate induced oxidative stress and DNA damage in Clarias gariepinus. AU - Soliman,Hamdy A M, AU - Hamed,Mohamed, AU - Lee,Jae-Seong, AU - Sayed,Alaa El-Din H, Y1 - 2019/01/25/ PY - 2018/06/20/received PY - 2019/01/15/revised PY - 2019/01/20/accepted PY - 2019/2/4/pubmed PY - 2019/4/4/medline PY - 2019/2/4/entrez KW - Catfish KW - Hepatotoxic KW - LDH KW - Lead nitrate KW - Pyrazole-5-carboxamide KW - TAC SP - 678 EP - 684 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 247 N2 - Pyrazole derivatives display diverse biological and pharmacological activities. The aim of this study is to investigate the antioxidant properties of a novel pyrazolecarboxamide derivative (4-amino-N-[(4-chlorophenyl)]-3-methyl-1-phenyl-1H-thieno [2, 3-c] pyrazole-5-carboxamide) in African catfish, Clarias gariepinus, exposed to 1 mg/L PbNO3. Fish were intramuscularly injected with pyrazole-5-carboxamidederivative according to the following groupings: Group 1 (control), Group 2 (1 mg/L lead nitrate), Group 3 (1 mg/L lead nitrate + 5 mg pyrazole derivative/kg body weight), and Group 4 (1 mg/L lead nitrate + 10 mg pyrazole derivative/kg body weight) for two weeks and four weeks. Lead nitrate (1 mg/L) caused significant elevation of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, uric acid, cholesterol, and glucose-6-phosphate dehydrogenase (G6PDH) compared to the control group after two and four weeks of exposure, while serum total lipids, alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were significantly reduced compared to the control group. Furthermore, levels of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and total antioxidant capacity (TAC) were reduced in group 2 compared to the control group. However, in group 2, hepatic lipid peroxidation (LPO) and DNA fragmentation percentage were significantly increased compared to the control group. Histopathological changes in the liver of lead-exposed groups included marked disturbance of hepatic tissue organization, degeneration of hepatocytes, dilation of blood sinusoids and the central vein as well as necrosis. Injection of pyrazole derivative for two weeks and four weeks reversed alterations in biochemical parameters, antioxidant biomarkers, lipid peroxidation, hepatic DNA damage, and histopathological changes in liver tissue induced by 1 mg/L lead nitrate. This amelioration was higher in response to high-dose pyrazole derivative (10 mg) at the fourth week of exposure, showing concentration-and time-dependency. Overall, the sensitized derivative pyrazolecarboxamide is likely a useful tool to minimize the effects of lead toxicity due to its potent antioxidant activity. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/30711823/Protective_effects_of_a_novel_pyrazolecarboxamide_derivative_against_lead_nitrate_induced_oxidative_stress_and_DNA_damage_in_Clarias_gariepinus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(18)32832-X DB - PRIME DP - Unbound Medicine ER -