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Effect of long-term and short-term diabetes on the parathyroid hormone sensitive rat renal adenylate cyclase: correlation with vitamin D metabolism.
Can J Physiol Pharmacol. 1988 Oct; 66(10):1313-8.CJ

Abstract

In short-term experiments, male Wistar rats were made diabetic for 10 days with a single injection of streptozotocin (65 mg/kg body weight). One group of diabetic rats was treated with insulin for 3 days prior to sacrifice. In long-term experiments, vitamin D replete or vitamin D depleted rats were made diabetic for 6 weeks. Criteria for diabetes were loss of weight, glycosuria (Tes-Tape), and hyperglycemia. In long-term diabetic rats the activity of renal mitochondrial 25-hydroxyvitamin D3 (25-(OH)D3) 1 alpha-hydroxylase was significantly decreased and that of 25-(OH)D3 24-hydroxylase increased. However, the parathyroid hormone (PTH) sensitive renal adenylate cyclase activity of diabetic rats was not different from that of the nondiabetic rats in either the vitamin D replete group or the vitamin D depleted group. On the other hand, the PTH-sensitive renal adenylate cyclase activity was significantly higher in short-term diabetic rats than in control and insulin-treated rats. These differences were observed at doses of 10(-8) to 10(-5) M of PTH. This study has demonstrated for the first time that there are differences in the PTH-sensitive adenylate cyclase response between long-term and short-term diabetic rats. The hypersensitivity to PTH of the renal adenylate cyclase observed in short-term diabetic rats probably represents a response to insulin deficiency during the early development of diabetes mellitus in the rats.

Authors+Show Affiliations

Division of Endocrinology and Metabolism, St. Michael's Hospital, Toronto, Ont., Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3071395

Citation

Rao, L G., and M S. Kung. "Effect of Long-term and Short-term Diabetes On the Parathyroid Hormone Sensitive Rat Renal Adenylate Cyclase: Correlation With Vitamin D Metabolism." Canadian Journal of Physiology and Pharmacology, vol. 66, no. 10, 1988, pp. 1313-8.
Rao LG, Kung MS. Effect of long-term and short-term diabetes on the parathyroid hormone sensitive rat renal adenylate cyclase: correlation with vitamin D metabolism. Can J Physiol Pharmacol. 1988;66(10):1313-8.
Rao, L. G., & Kung, M. S. (1988). Effect of long-term and short-term diabetes on the parathyroid hormone sensitive rat renal adenylate cyclase: correlation with vitamin D metabolism. Canadian Journal of Physiology and Pharmacology, 66(10), 1313-8.
Rao LG, Kung MS. Effect of Long-term and Short-term Diabetes On the Parathyroid Hormone Sensitive Rat Renal Adenylate Cyclase: Correlation With Vitamin D Metabolism. Can J Physiol Pharmacol. 1988;66(10):1313-8. PubMed PMID: 3071395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of long-term and short-term diabetes on the parathyroid hormone sensitive rat renal adenylate cyclase: correlation with vitamin D metabolism. AU - Rao,L G, AU - Kung,M S, PY - 1988/10/1/pubmed PY - 1988/10/1/medline PY - 1988/10/1/entrez SP - 1313 EP - 8 JF - Canadian journal of physiology and pharmacology JO - Can. J. Physiol. Pharmacol. VL - 66 IS - 10 N2 - In short-term experiments, male Wistar rats were made diabetic for 10 days with a single injection of streptozotocin (65 mg/kg body weight). One group of diabetic rats was treated with insulin for 3 days prior to sacrifice. In long-term experiments, vitamin D replete or vitamin D depleted rats were made diabetic for 6 weeks. Criteria for diabetes were loss of weight, glycosuria (Tes-Tape), and hyperglycemia. In long-term diabetic rats the activity of renal mitochondrial 25-hydroxyvitamin D3 (25-(OH)D3) 1 alpha-hydroxylase was significantly decreased and that of 25-(OH)D3 24-hydroxylase increased. However, the parathyroid hormone (PTH) sensitive renal adenylate cyclase activity of diabetic rats was not different from that of the nondiabetic rats in either the vitamin D replete group or the vitamin D depleted group. On the other hand, the PTH-sensitive renal adenylate cyclase activity was significantly higher in short-term diabetic rats than in control and insulin-treated rats. These differences were observed at doses of 10(-8) to 10(-5) M of PTH. This study has demonstrated for the first time that there are differences in the PTH-sensitive adenylate cyclase response between long-term and short-term diabetic rats. The hypersensitivity to PTH of the renal adenylate cyclase observed in short-term diabetic rats probably represents a response to insulin deficiency during the early development of diabetes mellitus in the rats. SN - 0008-4212 UR - https://www.unboundmedicine.com/medline/citation/3071395/Effect_of_long_term_and_short_term_diabetes_on_the_parathyroid_hormone_sensitive_rat_renal_adenylate_cyclase:_correlation_with_vitamin_D_metabolism_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/y88-215?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -