Tags

Type your tag names separated by a space and hit enter

Fabrication of a core-shell-shell magnetic polymeric microsphere with excellent performance for separation and purification of bromelain.
Food Chem 2019; 283:1-10FC

Abstract

Bromelain is an important industrial proteolytic enzyme which has great commercial value and is of wide application in food, beverage, tenderization, cosmetic, textile and pharmaceutical industries. In this work, the core-shell-shell magnetic polymeric microspheres (Fe3O4@SiO2@P(NIPAM-co-AIM)/Ni2+) composed of an SiO2-coated Fe3O4 magnetite core and a Ni2+-immobilized cross-linked poly (N-isopropyl acrylamide-co-propylimidazole) (NIPAM-co-AIM) shell were synthesized via distillation-precipitation polymerization. The Ni2+ cations in the magnetic polymeric microspheres shell provided docking sites for histidine and the microspheres exhibited excellent performance in the separation of bromelain with a binding capacity as high as 198 mg/g, and the recovery of enzyme activity could achieve 80%. It was found that the microspheres showed excellent performance for separation and purification of bromelain from the crude extract of pineapple peel, moreover the enzyme structure remained unchanged before and after elution process.

Authors+Show Affiliations

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.Henan Key Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan 467036, Henan, China.School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: yunwang@ujs.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30722847

Citation

Han, Juan, et al. "Fabrication of a Core-shell-shell Magnetic Polymeric Microsphere With Excellent Performance for Separation and Purification of Bromelain." Food Chemistry, vol. 283, 2019, pp. 1-10.
Han J, Wang L, Wang L, et al. Fabrication of a core-shell-shell magnetic polymeric microsphere with excellent performance for separation and purification of bromelain. Food Chem. 2019;283:1-10.
Han, J., Wang, L., Wang, L., Li, C., Mao, Y., & Wang, Y. (2019). Fabrication of a core-shell-shell magnetic polymeric microsphere with excellent performance for separation and purification of bromelain. Food Chemistry, 283, pp. 1-10. doi:10.1016/j.foodchem.2019.01.016.
Han J, et al. Fabrication of a Core-shell-shell Magnetic Polymeric Microsphere With Excellent Performance for Separation and Purification of Bromelain. Food Chem. 2019 Jun 15;283:1-10. PubMed PMID: 30722847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication of a core-shell-shell magnetic polymeric microsphere with excellent performance for separation and purification of bromelain. AU - Han,Juan, AU - Wang,Li, AU - Wang,Lei, AU - Li,Chunmei, AU - Mao,Yanli, AU - Wang,Yun, Y1 - 2019/01/14/ PY - 2018/08/10/received PY - 2018/11/22/revised PY - 2019/01/03/accepted PY - 2019/2/7/entrez PY - 2019/2/7/pubmed PY - 2019/3/26/medline KW - Bromelain KW - Core-shell-shell structure KW - Magnetic polymeric microspheres KW - Separation and purification SP - 1 EP - 10 JF - Food chemistry JO - Food Chem VL - 283 N2 - Bromelain is an important industrial proteolytic enzyme which has great commercial value and is of wide application in food, beverage, tenderization, cosmetic, textile and pharmaceutical industries. In this work, the core-shell-shell magnetic polymeric microspheres (Fe3O4@SiO2@P(NIPAM-co-AIM)/Ni2+) composed of an SiO2-coated Fe3O4 magnetite core and a Ni2+-immobilized cross-linked poly (N-isopropyl acrylamide-co-propylimidazole) (NIPAM-co-AIM) shell were synthesized via distillation-precipitation polymerization. The Ni2+ cations in the magnetic polymeric microspheres shell provided docking sites for histidine and the microspheres exhibited excellent performance in the separation of bromelain with a binding capacity as high as 198 mg/g, and the recovery of enzyme activity could achieve 80%. It was found that the microspheres showed excellent performance for separation and purification of bromelain from the crude extract of pineapple peel, moreover the enzyme structure remained unchanged before and after elution process. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/30722847/Fabrication_of_a_core_shell_shell_magnetic_polymeric_microsphere_with_excellent_performance_for_separation_and_purification_of_bromelain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(19)30054-8 DB - PRIME DP - Unbound Medicine ER -