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Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi.
Pest Manag Sci. 2019 Oct; 75(10):2652-2662.PM

Abstract

BACKGROUND

One concern with the adoption of RNAi-based genetically engineered (GE) crops is the potential harm to valued non-target organisms. Species of Coccinellidae (Coleoptera) are important natural enemies and might be exposed to the insecticidal dsRNA produced by the plant. To assess their susceptibility to dietary RNAi, we fed Adalia bipunctata and Coccinella septempunctata with a dsRNA designed to target the vATPase A of the western corn rootworm, Diabrotica virgifera virgifera (Dvv dsRNA). Specific dsRNAs designed to target the vATPase A of the two ladybird beetle species served as positive controls.

RESULTS

Our results revealed that both species were sensitive to dietary RNAi when ingesting their own dsRNAs, with C. septempunctata being more sensitive than A. bipunctata. Dvv dsRNA also adversely affected the two ladybird beetles as indicated by a significantly (but marginally) prolonged developmental time for A. bipunctata and a significantly reduced survival rate for C. septempunctata. These results, however, were obtained at Dvv dsRNA concentrations that were orders of magnitude higher than expected to occur in the field. Gene expression analyses confirmed the bioactivity of the dsRNA treatments and the results from the feeding bioassays. These results are consistent with the bioinformatics analyses, which revealed a higher number of 21-nucleotide-long matches, a requirement for effective RNAi, of the Dvv dsRNA with the vATPase A of C. septempunctata (34 matches) than with that of A. bipunctata (six matches).

CONCLUSION

Feeding bioassays revealed that two ladybird species are responsive to dietary RNAi. The two species, however, differed in their sensitivity. © 2019 Society of Chemical Industry.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Haller S
Research Division Agroecology and Environment, Agroscope, Zurich, Switzerland.
Widmer F
Competence Division Method Development and Analytics, Agroscope, Zurich, Switzerland.
Siegfried BD
Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA.
Zhuo X
Department of Entomology, University of Kentucky, Lexington, KY, USA.
Romeis J
Research Division Agroecology and Environment, Agroscope, Zurich, Switzerland.

MeSH

AnimalsColeopteraDietInsect ControlMothsRNA InterferenceRNA, Double-StrandedSpecies Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30729648

Citation

Haller, Simone, et al. "Responses of Two Ladybird Beetle Species (Coleoptera: Coccinellidae) to Dietary RNAi." Pest Management Science, vol. 75, no. 10, 2019, pp. 2652-2662.
Haller S, Widmer F, Siegfried BD, et al. Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi. Pest Manag Sci. 2019;75(10):2652-2662.
Haller, S., Widmer, F., Siegfried, B. D., Zhuo, X., & Romeis, J. (2019). Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi. Pest Management Science, 75(10), 2652-2662. https://doi.org/10.1002/ps.5370
Haller S, et al. Responses of Two Ladybird Beetle Species (Coleoptera: Coccinellidae) to Dietary RNAi. Pest Manag Sci. 2019;75(10):2652-2662. PubMed PMID: 30729648.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi. AU - Haller,Simone, AU - Widmer,Franco, AU - Siegfried,Blair D, AU - Zhuo,Xuguo, AU - Romeis,Jörg, Y1 - 2019/03/20/ PY - 2018/10/22/received PY - 2019/01/10/revised PY - 2019/02/04/accepted PY - 2019/2/8/pubmed PY - 2019/12/18/medline PY - 2019/2/8/entrez KW - Adalia bipunctata KW - Coccinella septempunctata KW - Diabrotica virgifera virgifera KW - dsRNA KW - environmental risk assessment KW - non-target effects KW - vATPase A SP - 2652 EP - 2662 JF - Pest management science JO - Pest Manag Sci VL - 75 IS - 10 N2 - BACKGROUND: One concern with the adoption of RNAi-based genetically engineered (GE) crops is the potential harm to valued non-target organisms. Species of Coccinellidae (Coleoptera) are important natural enemies and might be exposed to the insecticidal dsRNA produced by the plant. To assess their susceptibility to dietary RNAi, we fed Adalia bipunctata and Coccinella septempunctata with a dsRNA designed to target the vATPase A of the western corn rootworm, Diabrotica virgifera virgifera (Dvv dsRNA). Specific dsRNAs designed to target the vATPase A of the two ladybird beetle species served as positive controls. RESULTS: Our results revealed that both species were sensitive to dietary RNAi when ingesting their own dsRNAs, with C. septempunctata being more sensitive than A. bipunctata. Dvv dsRNA also adversely affected the two ladybird beetles as indicated by a significantly (but marginally) prolonged developmental time for A. bipunctata and a significantly reduced survival rate for C. septempunctata. These results, however, were obtained at Dvv dsRNA concentrations that were orders of magnitude higher than expected to occur in the field. Gene expression analyses confirmed the bioactivity of the dsRNA treatments and the results from the feeding bioassays. These results are consistent with the bioinformatics analyses, which revealed a higher number of 21-nucleotide-long matches, a requirement for effective RNAi, of the Dvv dsRNA with the vATPase A of C. septempunctata (34 matches) than with that of A. bipunctata (six matches). CONCLUSION: Feeding bioassays revealed that two ladybird species are responsive to dietary RNAi. The two species, however, differed in their sensitivity. © 2019 Society of Chemical Industry. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/30729648/Responses_of_two_ladybird_beetle_species__Coleoptera:_Coccinellidae__to_dietary_RNAi_ DB - PRIME DP - Unbound Medicine ER -