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Structural, rheological and antioxidant properties of pectins from Equisetum arvense L. and Equisetum sylvaticum L.
Carbohydr Polym 2019; 209:239-249CP

Abstract

The pectins were isolated from sterile stems of E. arvense (EA, yield 5.9%) and E. sylvaticum (ES, yield 4.8%) (Equisetaceae) using ammonium oxalate extraction after preliminary treatment with dilute HCl (рH 4.0). The pectins possessed high molecular weight (Mw, 340-360 kDa), high GalA content (ca. 85%), low degrees of methyl-esterification (14-16%) and acetylation (3-8%). NMR analysis indicated extensive regions of partially methyl-etherified and 3-O-acetylated HG and minor regions of low branched RG in the fragment isolated after hydrolysis of pectin EA by pectinase. Pectin EA produced a higher viscosity solution, formed a stronger and more rigid ionotropic hydrogel than pectin ES. The pectins scavenged DPPH and hydroxyl radicals, but not the superoxide radical and hydrogen peroxide. Phenolic compounds (0.11 and 0.23%) associated with polysaccharide moieties were apparently responsible for the differences in the anti-DPPH scavenging activity of pectins EA and ES (63 and 49%). The findings suggested that pectin from E. arvense should be more perspective than pectin from E. sylvaticum on their use as components of wound healing remedies.

Authors+Show Affiliations

Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., Syktyvkar, 167982, Russia. Electronic address: patova_olga@mail.ru.Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., Syktyvkar, 167982, Russia.Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., Syktyvkar, 167982, Russia.Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., Syktyvkar, 167982, Russia.N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47, Leninsky Prospect, Moscow 119991, Russia.Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., Syktyvkar, 167982, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30732805

Citation

Patova, O A., et al. "Structural, Rheological and Antioxidant Properties of Pectins From Equisetum Arvense L. and Equisetum Sylvaticum L." Carbohydrate Polymers, vol. 209, 2019, pp. 239-249.
Patova OA, Smirnov VV, Golovchenko VV, et al. Structural, rheological and antioxidant properties of pectins from Equisetum arvense L. and Equisetum sylvaticum L. Carbohydr Polym. 2019;209:239-249.
Patova, O. A., Smirnov, V. V., Golovchenko, V. V., Vityazev, F. V., Shashkov, A. S., & Popov, S. V. (2019). Structural, rheological and antioxidant properties of pectins from Equisetum arvense L. and Equisetum sylvaticum L. Carbohydrate Polymers, 209, pp. 239-249. doi:10.1016/j.carbpol.2018.12.098.
Patova OA, et al. Structural, Rheological and Antioxidant Properties of Pectins From Equisetum Arvense L. and Equisetum Sylvaticum L. Carbohydr Polym. 2019 Apr 1;209:239-249. PubMed PMID: 30732805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural, rheological and antioxidant properties of pectins from Equisetum arvense L. and Equisetum sylvaticum L. AU - Patova,O A, AU - Smirnov,V V, AU - Golovchenko,V V, AU - Vityazev,F V, AU - Shashkov,A S, AU - Popov,S V, Y1 - 2019/01/02/ PY - 2018/09/06/received PY - 2018/12/21/revised PY - 2018/12/30/accepted PY - 2019/2/9/entrez PY - 2019/2/9/pubmed PY - 2019/2/9/medline KW - 3,5-Xylenol (PubChem CID: 7948) KW - Calcium chloride (PubChem CID: 5284359) KW - Characterization KW - Ethanol (PubChem CID: 702) KW - Horsetails KW - Hydrochloric acid (PubChem CID: 313) KW - Hydrogen peroxide (PubChem CID: 784) KW - NMR KW - Pectin KW - Radical scavenging activity KW - Sodium chloride (PubChem CID: 5234) KW - Textural property KW - Trolox (PubChem CID: 40634) KW - Xanthine (PubChem CID: 1188) KW - d-galacturonic acid (PubChem CID: 439215) SP - 239 EP - 249 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 209 N2 - The pectins were isolated from sterile stems of E. arvense (EA, yield 5.9%) and E. sylvaticum (ES, yield 4.8%) (Equisetaceae) using ammonium oxalate extraction after preliminary treatment with dilute HCl (рH 4.0). The pectins possessed high molecular weight (Mw, 340-360 kDa), high GalA content (ca. 85%), low degrees of methyl-esterification (14-16%) and acetylation (3-8%). NMR analysis indicated extensive regions of partially methyl-etherified and 3-O-acetylated HG and minor regions of low branched RG in the fragment isolated after hydrolysis of pectin EA by pectinase. Pectin EA produced a higher viscosity solution, formed a stronger and more rigid ionotropic hydrogel than pectin ES. The pectins scavenged DPPH and hydroxyl radicals, but not the superoxide radical and hydrogen peroxide. Phenolic compounds (0.11 and 0.23%) associated with polysaccharide moieties were apparently responsible for the differences in the anti-DPPH scavenging activity of pectins EA and ES (63 and 49%). The findings suggested that pectin from E. arvense should be more perspective than pectin from E. sylvaticum on their use as components of wound healing remedies. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/30732805/Structural,_rheological_and_antioxidant_properties_of_pectins_from_Equisetum_arvense_L._and_Equisetum_sylvaticum_L L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(18)31558-3 DB - PRIME DP - Unbound Medicine ER -