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Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria.
J Appl Microbiol 2019; 126(5):1353-1361JA

Abstract

AIMS

This study investigated the in vitro antibacterial activity of Hesperozygis ringens (Benth.) Epling leaf extracts against fish pathogenic bacteria, as well as the in vivo activity of the most active extract in silver catfish (Rhamdia quelen) experimentally infected with Aeromonas hydrophila. Moreover, the chemical composition of the extract used in the survival assay was evaluated.

METHODS AND RESULTS

Only hexane extract (HEHR) showed in vitro antibacterial activity (MIC and MBC ranging from 1600 to 3200 μg ml-1) against clinical isolates of A. hydrophila, Raoultella ornithinolytica and Citrobacter freundii, obtained from naturally infected silver catfish, and A. hydrophilaATCC 7966. The major compound of the volatile fraction of HEHR was determined as pulegone. HEHR promoted a 93·33% relative survival rate of silver catfish experimentally infected with A. hydrophila 7 days after a single therapeutic bath at 30 mg l-1 , while florfenicol at 4 mg l-1 , which promoted a 60% relative survival rate.

CONCLUSIONS

The antibacterial activity of H. ringens (Benth.) Epling leaf extracts seems to be related to phytochemicals of apolar character, since HEHR promoted better survival rate of infected animals than florfenicol.

SIGNIFICANCE AND IMPACT OF THE STUDY

The HEHR has potential to be used in the control and treatment of bacterial infections in organic aquaculture.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30735293

Citation

Rosa, I A., et al. "Extracts of Hesperozygis Ringens (Benth.) Epling: in Vitro and in Vivo Antibacterial Activity Against Fish Pathogenic Bacteria." Journal of Applied Microbiology, vol. 126, no. 5, 2019, pp. 1353-1361.
Rosa IA, Rodrigues P, Bianchini AE, et al. Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria. J Appl Microbiol. 2019;126(5):1353-1361.
Rosa, I. A., Rodrigues, P., Bianchini, A. E., Silveira, B. P., Ferrari, F. T., Bandeira Junior, G., ... Heinzmann, B. M. (2019). Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria. Journal of Applied Microbiology, 126(5), pp. 1353-1361. doi:10.1111/jam.14219.
Rosa IA, et al. Extracts of Hesperozygis Ringens (Benth.) Epling: in Vitro and in Vivo Antibacterial Activity Against Fish Pathogenic Bacteria. J Appl Microbiol. 2019;126(5):1353-1361. PubMed PMID: 30735293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria. AU - Rosa,I A, AU - Rodrigues,P, AU - Bianchini,A E, AU - Silveira,B P, AU - Ferrari,F T, AU - Bandeira Junior,G, AU - Vargas,A P C, AU - Baldisserotto,B, AU - Heinzmann,B M, Y1 - 2019/03/19/ PY - 2018/10/02/received PY - 2019/01/10/revised PY - 2019/01/29/accepted PY - 2019/2/9/pubmed PY - 2019/5/22/medline PY - 2019/2/9/entrez KW - antibiotics KW - aquaculture KW - diseases KW - fish (live) KW - infection SP - 1353 EP - 1361 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 126 IS - 5 N2 - AIMS: This study investigated the in vitro antibacterial activity of Hesperozygis ringens (Benth.) Epling leaf extracts against fish pathogenic bacteria, as well as the in vivo activity of the most active extract in silver catfish (Rhamdia quelen) experimentally infected with Aeromonas hydrophila. Moreover, the chemical composition of the extract used in the survival assay was evaluated. METHODS AND RESULTS: Only hexane extract (HEHR) showed in vitro antibacterial activity (MIC and MBC ranging from 1600 to 3200 μg ml-1) against clinical isolates of A. hydrophila, Raoultella ornithinolytica and Citrobacter freundii, obtained from naturally infected silver catfish, and A. hydrophilaATCC 7966. The major compound of the volatile fraction of HEHR was determined as pulegone. HEHR promoted a 93·33% relative survival rate of silver catfish experimentally infected with A. hydrophila 7 days after a single therapeutic bath at 30 mg l-1 , while florfenicol at 4 mg l-1 , which promoted a 60% relative survival rate. CONCLUSIONS: The antibacterial activity of H. ringens (Benth.) Epling leaf extracts seems to be related to phytochemicals of apolar character, since HEHR promoted better survival rate of infected animals than florfenicol. SIGNIFICANCE AND IMPACT OF THE STUDY: The HEHR has potential to be used in the control and treatment of bacterial infections in organic aquaculture. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/30735293/Extracts_of_Hesperozygis_ringens__Benth___Epling:_in_vitro_and_in_vivo_antibacterial_activity_against_fish_pathogenic_bacteria_ L2 - https://doi.org/10.1111/jam.14219 DB - PRIME DP - Unbound Medicine ER -