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Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
Int J Biol Macromol 2019; 128:973-984IJ

Abstract

Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan. However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity. In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties. The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix. The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents. An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions. Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization. The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.

Authors+Show Affiliations

School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan; Department of orthopedics, Taipei Medical University Hospital, Taipei 11031, Taiwan.Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan.Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan. Electronic address: flmi530326@tmu.edu.tw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30738901

Citation

Lu, Hsien-Tsung, et al. "Development of Genipin-crosslinked and Fucoidan-adsorbed Nano-hydroxyapatite/hydroxypropyl Chitosan Composite Scaffolds for Bone Tissue Engineering." International Journal of Biological Macromolecules, vol. 128, 2019, pp. 973-984.
Lu HT, Lu TW, Chen CH, et al. Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering. Int J Biol Macromol. 2019;128:973-984.
Lu, H. T., Lu, T. W., Chen, C. H., & Mi, F. L. (2019). Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering. International Journal of Biological Macromolecules, 128, pp. 973-984. doi:10.1016/j.ijbiomac.2019.02.010.
Lu HT, et al. Development of Genipin-crosslinked and Fucoidan-adsorbed Nano-hydroxyapatite/hydroxypropyl Chitosan Composite Scaffolds for Bone Tissue Engineering. Int J Biol Macromol. 2019 May 1;128:973-984. PubMed PMID: 30738901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering. AU - Lu,Hsien-Tsung, AU - Lu,Tzu-Wei, AU - Chen,Chien-Ho, AU - Mi,Fwu-Long, Y1 - 2019/02/08/ PY - 2018/11/30/received PY - 2019/01/07/revised PY - 2019/02/02/accepted PY - 2019/2/11/pubmed PY - 2019/6/20/medline PY - 2019/2/11/entrez KW - Bone tissue engineering KW - Chitosan KW - Fucoidan KW - Genipin KW - Hydroxyapatite KW - Scaffolds SP - 973 EP - 984 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 128 N2 - Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan. However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity. In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties. The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix. The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents. An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions. Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization. The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30738901/Development_of_genipin_crosslinked_and_fucoidan_adsorbed_nano_hydroxyapatite/hydroxypropyl_chitosan_composite_scaffolds_for_bone_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)36607-8 DB - PRIME DP - Unbound Medicine ER -