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Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium tramdenum Bark.
Molecules. 2019 Feb 09; 24(3)M

Abstract

The fruits of Canarium tramdenum are commonly used as foods and cooking ingredients in Vietnam, Laos, and the southeast region of China, whilst the leaves are traditionally used for treating diarrhea and rheumatism. This study was conducted to investigate the potential use of this plant bark as antioxidants, and α-amylase and α-glucosidase inhibitors. Five different extracts of C. tramdenum bark (TDB) consisting of the extract (TDBS) and factional extracts hexane (TDBH), ethyl acetate (TDBE), butanol (TDBB), and water (TDBW) were evaluated. The TDBS extract contained the highest amount of total phenolic (112.14 mg gallic acid equivalent per g dry weight), while the TDBB extract had the most effective antioxidant capacity compared to other extracts. Its IC50 values were 12.33, 47.87, 33.25, and 103.74 µg/mL in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (ABTS), reducing power (RP), and nitric oxide (NO) assays, respectively. Meanwhile, the lipid peroxidation inhibition of the four above extracts was proximate to that of butylated hydroxytoluene (BHT) as a standard antioxidant. The result of porcine pancreatic α-amylase inhibition showed that TDB extracts have promising effects which are in line with the commercial diabetic inhibitor acarbose. Interestingly, the inhibitory ability on α-glucosidase of all the extracts was higher than that of acarbose. Among the extracts, the TDBB extract expressed the strongest activity on the enzymatic reaction (IC50 = 18.93 µg/mL) followed by the TDBW extract (IC50 = 25.27 µg/mL), TDBS (IC50 = 28.17 µg/mL), and TDBE extract (IC50 = 141.37 µg/mL). The phytochemical constituents of the TDB extract were identified by gas chromatography⁻mass spectrometry (GC-MS). The principal constituents included nine phenolics, eight terpenoids, two steroids, and five compounds belonging to other chemical classes, which were the first reported in this plant. Among them, the presence of α- and β-amyrins were identified by GC-MS and appeared as the most dominant constituents in TDB extracts (1.52 mg/g). The results of this study revealed that C. tramdenum bark possessed rich phenolics and terpenoids, which might confer on reducing risks from diabetes. A high quantity of α- and β-amyrins highlighted the potentials of anti-inflammatory, anti-ulcer, anti-hyperlipidemic, anti-tumor, and hepatoprotective properties of C. tramdenum bark.

Authors+Show Affiliations

Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan. nguyenquan26@gmail.com.Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan. tdxuan@hiroshima-u.ac.jp.Department of Biotechnology, NTT Institute of Hi-Technology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh 72820, Vietnam. thdung@ntt.edu.vn.Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan. dieuthuykttb@gmail.com.Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan. trangle9872@gmail.com.Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan. cth1412@gmail.com.Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan. yusufandriana@yahoo.com.Faculty of Forest Resources and Environmental Management, Vietnam National University of Forestry, Xuan Mai, Hanoi 156200 Vietnam. phungtuyen@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30744084

Citation

Quan, Nguyen Van, et al. "Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium Tramdenum Bark." Molecules (Basel, Switzerland), vol. 24, no. 3, 2019.
Quan NV, Xuan TD, Tran HD, et al. Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium tramdenum Bark. Molecules. 2019;24(3).
Quan, N. V., Xuan, T. D., Tran, H. D., Thuy, N. T. D., Trang, L. T., Huong, C. T., Andriana, Y., & Tuyen, P. T. (2019). Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium tramdenum Bark. Molecules (Basel, Switzerland), 24(3). https://doi.org/10.3390/molecules24030605
Quan NV, et al. Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium Tramdenum Bark. Molecules. 2019 Feb 9;24(3) PubMed PMID: 30744084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium tramdenum Bark. AU - Quan,Nguyen Van, AU - Xuan,Tran Dang, AU - Tran,Hoang-Dung, AU - Thuy,Nguyen Thi Dieu, AU - Trang,Le Thu, AU - Huong,Can Thu, AU - Andriana,Yusuf, AU - Tuyen,Phung Thi, Y1 - 2019/02/09/ PY - 2019/01/13/received PY - 2019/02/06/revised PY - 2019/02/07/accepted PY - 2019/2/13/entrez PY - 2019/2/13/pubmed PY - 2019/6/5/medline KW - Canarium tramdenum KW - antioxidants KW - bark KW - biological activity KW - diabetes KW - phenolics KW - terpenoids KW - α-glucosidase inhibitors JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 3 N2 - The fruits of Canarium tramdenum are commonly used as foods and cooking ingredients in Vietnam, Laos, and the southeast region of China, whilst the leaves are traditionally used for treating diarrhea and rheumatism. This study was conducted to investigate the potential use of this plant bark as antioxidants, and α-amylase and α-glucosidase inhibitors. Five different extracts of C. tramdenum bark (TDB) consisting of the extract (TDBS) and factional extracts hexane (TDBH), ethyl acetate (TDBE), butanol (TDBB), and water (TDBW) were evaluated. The TDBS extract contained the highest amount of total phenolic (112.14 mg gallic acid equivalent per g dry weight), while the TDBB extract had the most effective antioxidant capacity compared to other extracts. Its IC50 values were 12.33, 47.87, 33.25, and 103.74 µg/mL in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (ABTS), reducing power (RP), and nitric oxide (NO) assays, respectively. Meanwhile, the lipid peroxidation inhibition of the four above extracts was proximate to that of butylated hydroxytoluene (BHT) as a standard antioxidant. The result of porcine pancreatic α-amylase inhibition showed that TDB extracts have promising effects which are in line with the commercial diabetic inhibitor acarbose. Interestingly, the inhibitory ability on α-glucosidase of all the extracts was higher than that of acarbose. Among the extracts, the TDBB extract expressed the strongest activity on the enzymatic reaction (IC50 = 18.93 µg/mL) followed by the TDBW extract (IC50 = 25.27 µg/mL), TDBS (IC50 = 28.17 µg/mL), and TDBE extract (IC50 = 141.37 µg/mL). The phytochemical constituents of the TDB extract were identified by gas chromatography⁻mass spectrometry (GC-MS). The principal constituents included nine phenolics, eight terpenoids, two steroids, and five compounds belonging to other chemical classes, which were the first reported in this plant. Among them, the presence of α- and β-amyrins were identified by GC-MS and appeared as the most dominant constituents in TDB extracts (1.52 mg/g). The results of this study revealed that C. tramdenum bark possessed rich phenolics and terpenoids, which might confer on reducing risks from diabetes. A high quantity of α- and β-amyrins highlighted the potentials of anti-inflammatory, anti-ulcer, anti-hyperlipidemic, anti-tumor, and hepatoprotective properties of C. tramdenum bark. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30744084/Antioxidant_α_Amylase_and_α_Glucosidase_Inhibitory_Activities_and_Potential_Constituents_of_Canarium_tramdenum_Bark_ L2 - https://www.mdpi.com/resolver?pii=molecules24030605 DB - PRIME DP - Unbound Medicine ER -