Tags

Type your tag names separated by a space and hit enter

Solanum lycopersicum GOLDEN 2-LIKE 2 transcription factor affects fruit quality in a light- and auxin-dependent manner.
PLoS One. 2019; 14(2):e0212224.Plos

Abstract

Plastids are organelles responsible for essential aspects of plant development, including carbon fixation and synthesis of several secondary metabolites. Chloroplast differentiation and activity are highly regulated by light, and several proteins involved in these processes have been characterised. Such is the case of the GOLDEN 2-LIKE (GLK) transcription factors, which induces the expression of genes related to chloroplast differentiation and photosynthesis. The tomato (Solanum lycopersicum) genome harbours two copies of this gene, SlGLK1 and SlGLK2, each with distinct expression patterns. While the former predominates in leaves, the latter is mainly expressed in fruits, precisely at the pedicel region. During tomato domestication, the selection of fruits with uniform ripening fixed the mutation Slglk2, nowadays present in most cultivated varieties, what penalised fruit metabolic composition. In this study, we investigated how SlGLK2 is regulated by light, auxin and cytokinin and determined the effect of SlGLK2 on tocopherol (vitamin E) and sugar metabolism, which are components of the fruit nutritional and industrial quality. To achieve this, transcriptional profiling and biochemical analysis were performed throughout fruit development and ripening from SlGLK2, Slglk2, SlGLK2-overexpressing genotypes, as well as from phytochrome and hormonal deficient mutants. The results revealed that SlGLK2 expression is regulated by phytochrome-mediated light perception, yet this gene can induce chloroplast differentiation even in a phytochrome-independent manner. Moreover, auxin was found to be a negative regulator of SlGLK2 expression, while SlGLK2 enhances cytokinin responsiveness. Additionally, SlGLK2 enhanced chlorophyll content in immature green fruits, leading to an increment in tocopherol level in ripe fruits. Finally, SlGLK2 overexpression resulted in higher total soluble solid content, possibly by the regulation of sugar metabolism enzyme-encoding genes. The results obtained here shed light on the regulatory network that interconnects SlGLK2, phytohormones and light signal, promoting the plastidial activity and consequently, influencing the quality of tomato fruit.

Authors+Show Affiliations

Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Ciências Biológicas, Escola Superior de Agricultura ¨Luiz de Queiroz¨, Universidade de São Paulo, Piracicaba, SP, Brazil.Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30753245

Citation

Lupi, Alessandra Cavalcanti Duarte, et al. "Solanum Lycopersicum GOLDEN 2-LIKE 2 Transcription Factor Affects Fruit Quality in a Light- and Auxin-dependent Manner." PloS One, vol. 14, no. 2, 2019, pp. e0212224.
Lupi ACD, Lira BS, Gramegna G, et al. Solanum lycopersicum GOLDEN 2-LIKE 2 transcription factor affects fruit quality in a light- and auxin-dependent manner. PLoS One. 2019;14(2):e0212224.
Lupi, A. C. D., Lira, B. S., Gramegna, G., Trench, B., Alves, F. R. R., Demarco, D., Peres, L. E. P., Purgatto, E., Freschi, L., & Rossi, M. (2019). Solanum lycopersicum GOLDEN 2-LIKE 2 transcription factor affects fruit quality in a light- and auxin-dependent manner. PloS One, 14(2), e0212224. https://doi.org/10.1371/journal.pone.0212224
Lupi ACD, et al. Solanum Lycopersicum GOLDEN 2-LIKE 2 Transcription Factor Affects Fruit Quality in a Light- and Auxin-dependent Manner. PLoS One. 2019;14(2):e0212224. PubMed PMID: 30753245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solanum lycopersicum GOLDEN 2-LIKE 2 transcription factor affects fruit quality in a light- and auxin-dependent manner. AU - Lupi,Alessandra Cavalcanti Duarte, AU - Lira,Bruno Silvestre, AU - Gramegna,Giovanna, AU - Trench,Bruna, AU - Alves,Frederico Rocha Rodrigues, AU - Demarco,Diego, AU - Peres,Lazaro Eustáquio Pereira, AU - Purgatto,Eduardo, AU - Freschi,Luciano, AU - Rossi,Magdalena, Y1 - 2019/02/12/ PY - 2018/10/05/received PY - 2019/01/29/accepted PY - 2019/2/13/entrez PY - 2019/2/13/pubmed PY - 2019/11/14/medline SP - e0212224 EP - e0212224 JF - PloS one JO - PLoS One VL - 14 IS - 2 N2 - Plastids are organelles responsible for essential aspects of plant development, including carbon fixation and synthesis of several secondary metabolites. Chloroplast differentiation and activity are highly regulated by light, and several proteins involved in these processes have been characterised. Such is the case of the GOLDEN 2-LIKE (GLK) transcription factors, which induces the expression of genes related to chloroplast differentiation and photosynthesis. The tomato (Solanum lycopersicum) genome harbours two copies of this gene, SlGLK1 and SlGLK2, each with distinct expression patterns. While the former predominates in leaves, the latter is mainly expressed in fruits, precisely at the pedicel region. During tomato domestication, the selection of fruits with uniform ripening fixed the mutation Slglk2, nowadays present in most cultivated varieties, what penalised fruit metabolic composition. In this study, we investigated how SlGLK2 is regulated by light, auxin and cytokinin and determined the effect of SlGLK2 on tocopherol (vitamin E) and sugar metabolism, which are components of the fruit nutritional and industrial quality. To achieve this, transcriptional profiling and biochemical analysis were performed throughout fruit development and ripening from SlGLK2, Slglk2, SlGLK2-overexpressing genotypes, as well as from phytochrome and hormonal deficient mutants. The results revealed that SlGLK2 expression is regulated by phytochrome-mediated light perception, yet this gene can induce chloroplast differentiation even in a phytochrome-independent manner. Moreover, auxin was found to be a negative regulator of SlGLK2 expression, while SlGLK2 enhances cytokinin responsiveness. Additionally, SlGLK2 enhanced chlorophyll content in immature green fruits, leading to an increment in tocopherol level in ripe fruits. Finally, SlGLK2 overexpression resulted in higher total soluble solid content, possibly by the regulation of sugar metabolism enzyme-encoding genes. The results obtained here shed light on the regulatory network that interconnects SlGLK2, phytohormones and light signal, promoting the plastidial activity and consequently, influencing the quality of tomato fruit. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30753245/Solanum_lycopersicum_GOLDEN_2_LIKE_2_transcription_factor_affects_fruit_quality_in_a_light__and_auxin_dependent_manner_ L2 - https://dx.plos.org/10.1371/journal.pone.0212224 DB - PRIME DP - Unbound Medicine ER -