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Correlation of creatinine- and specific gravity-normalized free and glucuronidated urine cannabinoid concentrations following smoked, vaporized, and oral cannabis in frequent and occasional cannabis users.
Drug Test Anal. 2019 Jul; 11(7):968-975.DT

Abstract

Variability in urine dilution complicates urine cannabinoid test interpretation. Normalizing urine cannabinoid concentrations to specific gravity (SG) or creatinine was proposed to account for donors' hydration states. In this study, all urine voids were individually collected from eight frequent and eight occasional cannabis users for up to 85 hours after each received on separate occasions 50.6 mg Δ9-tetrahydrocannabinol (THC) by smoking, vaporization, and oral ingestion in a randomized, within-subject, double-blind, double-dummy, placebo-controlled protocol. Each urine void was analyzed for 11 cannabinoids and phase I and II metabolites by liquid chromatography-tandem mass spectrometry (LC-MS/MS), SG, and creatinine. Normalized urine concentrations were log10 transformed to create normal distributions, and Pearson correlation coefficients determined the degree of association between the two normalization methods. Repeated-measures linear regression determined if the degree of association differed by frequent or occasional cannabis use, or route of administration after adjusting for gender and time since dosing. Of 1880 urine samples examined, only 11-nor-9-carboxy-THC (THCCOOH), THCCOOH-glucuronide, THC-glucuronide, and 11-nor-9-carboxy-Δ9-tetrahydrocannabivarin (THCVCOOH) were greater than the method's limits of quantification (LOQs). Associations between SG- and creatinine-normalized concentrations exceeded 0.90. Repeated-measures regression analysis found small but statistically significant differences in the degree of association between normalization methods for THCCOOH and THCCOOH-glucuronide in frequent vs occasional smokers, and in THCVCOOH and THC-glucuronide by route of administration. For the first time, SG- and creatinine-normalized urine cannabinoid concentrations were evaluated in frequent and occasional cannabis users and following oral, smoked, and inhaled cannabis. Both normalization methods reduced variability, improving the interpretation of urine cannabinoid concentrations and methods were strongly correlated.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, 21124, USA. Currently at The Lambert Center for the Study of Medicinal Cannabis and Hemp, Institute on Emerging Health Professions, Thomas Jefferson University, Philadelphia, PA, 19107, USA.Division of Laboratory Sciences, Tobacco and Volatiles Branch, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, 30341, USA.Division of Laboratory Sciences, Tobacco and Volatiles Branch, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, 30341, USA.Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, 21124, USA. Currently at the Office of the Chief Medical Examiner, Maryland Department of Health, Baltimore, MD, 21223, USA.Office of the Clinical Director, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, 21124, USA.Huestis and Smith Toxicology, LLC, Severna Park, MD, 21146, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30756523

Citation

Huestis, Marilyn A., et al. "Correlation of Creatinine- and Specific Gravity-normalized Free and Glucuronidated Urine Cannabinoid Concentrations Following Smoked, Vaporized, and Oral Cannabis in Frequent and Occasional Cannabis Users." Drug Testing and Analysis, vol. 11, no. 7, 2019, pp. 968-975.
Huestis MA, Blount BC, Milan DF, et al. Correlation of creatinine- and specific gravity-normalized free and glucuronidated urine cannabinoid concentrations following smoked, vaporized, and oral cannabis in frequent and occasional cannabis users. Drug Test Anal. 2019;11(7):968-975.
Huestis, M. A., Blount, B. C., Milan, D. F., Newmeyer, M. N., Schroeder, J., & Smith, M. L. (2019). Correlation of creatinine- and specific gravity-normalized free and glucuronidated urine cannabinoid concentrations following smoked, vaporized, and oral cannabis in frequent and occasional cannabis users. Drug Testing and Analysis, 11(7), 968-975. https://doi.org/10.1002/dta.2576
Huestis MA, et al. Correlation of Creatinine- and Specific Gravity-normalized Free and Glucuronidated Urine Cannabinoid Concentrations Following Smoked, Vaporized, and Oral Cannabis in Frequent and Occasional Cannabis Users. Drug Test Anal. 2019;11(7):968-975. PubMed PMID: 30756523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation of creatinine- and specific gravity-normalized free and glucuronidated urine cannabinoid concentrations following smoked, vaporized, and oral cannabis in frequent and occasional cannabis users. AU - Huestis,Marilyn A, AU - Blount,Benjamin C, AU - Milan,Daniel F, AU - Newmeyer,Matthew N, AU - Schroeder,Jennifer, AU - Smith,Michael L, Y1 - 2019/03/15/ PY - 2018/12/22/received PY - 2019/02/06/revised PY - 2019/02/07/accepted PY - 2019/2/14/pubmed PY - 2020/1/22/medline PY - 2019/2/14/entrez KW - creatinine KW - specific gravity KW - urine cannabinoids SP - 968 EP - 975 JF - Drug testing and analysis JO - Drug Test Anal VL - 11 IS - 7 N2 - Variability in urine dilution complicates urine cannabinoid test interpretation. Normalizing urine cannabinoid concentrations to specific gravity (SG) or creatinine was proposed to account for donors' hydration states. In this study, all urine voids were individually collected from eight frequent and eight occasional cannabis users for up to 85 hours after each received on separate occasions 50.6 mg Δ9-tetrahydrocannabinol (THC) by smoking, vaporization, and oral ingestion in a randomized, within-subject, double-blind, double-dummy, placebo-controlled protocol. Each urine void was analyzed for 11 cannabinoids and phase I and II metabolites by liquid chromatography-tandem mass spectrometry (LC-MS/MS), SG, and creatinine. Normalized urine concentrations were log10 transformed to create normal distributions, and Pearson correlation coefficients determined the degree of association between the two normalization methods. Repeated-measures linear regression determined if the degree of association differed by frequent or occasional cannabis use, or route of administration after adjusting for gender and time since dosing. Of 1880 urine samples examined, only 11-nor-9-carboxy-THC (THCCOOH), THCCOOH-glucuronide, THC-glucuronide, and 11-nor-9-carboxy-Δ9-tetrahydrocannabivarin (THCVCOOH) were greater than the method's limits of quantification (LOQs). Associations between SG- and creatinine-normalized concentrations exceeded 0.90. Repeated-measures regression analysis found small but statistically significant differences in the degree of association between normalization methods for THCCOOH and THCCOOH-glucuronide in frequent vs occasional smokers, and in THCVCOOH and THC-glucuronide by route of administration. For the first time, SG- and creatinine-normalized urine cannabinoid concentrations were evaluated in frequent and occasional cannabis users and following oral, smoked, and inhaled cannabis. Both normalization methods reduced variability, improving the interpretation of urine cannabinoid concentrations and methods were strongly correlated. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/30756523/Correlation_of_creatinine__and_specific_gravity_normalized_free_and_glucuronidated_urine_cannabinoid_concentrations_following_smoked_vaporized_and_oral_cannabis_in_frequent_and_occasional_cannabis_users_ L2 - https://doi.org/10.1002/dta.2576 DB - PRIME DP - Unbound Medicine ER -