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HR-LC-ESI-Orbitrap-MS based metabolite profiling of Prunus dulcis Mill. (Italian cultivars Toritto and Avola) husks and evaluation of antioxidant activity.
Phytochem Anal. 2019 Jul; 30(4):415-423.PA

Abstract

INTRODUCTION

Prunus dulcis Mill. is a plant cultivated for the production of its edible seeds, known as sweet almonds. In the last decades almond demand has highly increased due to their wide use in the industrial manufacturing of almond-based foods; this has led to a huge production of waste materials, mainly corresponding to shells and husks.

OBJECTIVE

To achieve a deeper understanding of the chemical composition of almond husks with the aim of exploring these byproducts as a source of bioactives to be used in nutraceutical and cosmetic formulations.

METHODS

Methanol, ethanol and ethanol-water extracts of the almond husks were analysed by high-resolution liquid chromatography electrospray ionisation Orbitrap mass spectrometry (HR-LC-ESI-Orbitrap-MS) in negative ion mode. Tandem mass spectrometry (MS/MS) data were acquired by using the Data-Dependent Scan experiment, allowing the precursor ion to be selected as the most intense peak during LC-MS analyses. Nuclear magnetic resonance (NMR) experiments were performed on a Bruker DRX-600 spectrometer. Folin-Ciocalteu, DPPH• (2,2-diphenyl-1-picrylhydrazyl) and TEAC (Trolox equivalent antioxidant capacity) assays were employed to determine the total phenolic content and the radical scavenging activity of the extracts.

RESULTS

The LC-MS/MS analysis of the methanol extract guided the isolation of phloroglucinol derivatives, flavonoids and terpenes. Eco-friendly extraction methods showed to be selective in extracting flavonoids while the comparison of the LC-MS profiles of the Italian cultivars Toritto and Avola showed significant differences, confirming how different growing conditions may influence the metabolome of a plant species.

CONCLUSION

This study led to a deeper insight into the chemical constituents of almond husks and showed how the eco-friendly extraction resulted in an effective method to obtain extracts rich in antioxidant sources.

Authors+Show Affiliations

Dipartimento di Farmacia, Università degli Studi di Salerno, Fisciano, SA, Italy. PhD Program in Drug Discovery and Development, University of Salerno, Fisciano, SA, Italy.Dipartimento di Farmacia, Università degli Studi di Salerno, Fisciano, SA, Italy.Dipartimento di Farmacia, Università degli Studi di Salerno, Fisciano, SA, Italy.Dipartimento di Farmacia, Università degli Studi di Salerno, Fisciano, SA, Italy.Dipartimento di Farmacia, Università degli Studi di Salerno, Fisciano, SA, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30762260

Citation

Bottone, Alfredo, et al. "HR-LC-ESI-Orbitrap-MS Based Metabolite Profiling of Prunus Dulcis Mill. (Italian Cultivars Toritto and Avola) Husks and Evaluation of Antioxidant Activity." Phytochemical Analysis : PCA, vol. 30, no. 4, 2019, pp. 415-423.
Bottone A, Masullo M, Montoro P, et al. HR-LC-ESI-Orbitrap-MS based metabolite profiling of Prunus dulcis Mill. (Italian cultivars Toritto and Avola) husks and evaluation of antioxidant activity. Phytochem Anal. 2019;30(4):415-423.
Bottone, A., Masullo, M., Montoro, P., Pizza, C., & Piacente, S. (2019). HR-LC-ESI-Orbitrap-MS based metabolite profiling of Prunus dulcis Mill. (Italian cultivars Toritto and Avola) husks and evaluation of antioxidant activity. Phytochemical Analysis : PCA, 30(4), 415-423. https://doi.org/10.1002/pca.2824
Bottone A, et al. HR-LC-ESI-Orbitrap-MS Based Metabolite Profiling of Prunus Dulcis Mill. (Italian Cultivars Toritto and Avola) Husks and Evaluation of Antioxidant Activity. Phytochem Anal. 2019;30(4):415-423. PubMed PMID: 30762260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HR-LC-ESI-Orbitrap-MS based metabolite profiling of Prunus dulcis Mill. (Italian cultivars Toritto and Avola) husks and evaluation of antioxidant activity. AU - Bottone,Alfredo, AU - Masullo,Milena, AU - Montoro,Paola, AU - Pizza,Cosimo, AU - Piacente,Sonia, Y1 - 2019/02/14/ PY - 2018/10/19/received PY - 2018/12/11/revised PY - 2019/01/09/accepted PY - 2019/2/15/pubmed PY - 2019/11/19/medline PY - 2019/2/15/entrez KW - HR-LC-ESI-Orbitrap-MS KW - Prunus dulcis KW - antioxidant activity KW - phenolics KW - terpenes SP - 415 EP - 423 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 30 IS - 4 N2 - INTRODUCTION: Prunus dulcis Mill. is a plant cultivated for the production of its edible seeds, known as sweet almonds. In the last decades almond demand has highly increased due to their wide use in the industrial manufacturing of almond-based foods; this has led to a huge production of waste materials, mainly corresponding to shells and husks. OBJECTIVE: To achieve a deeper understanding of the chemical composition of almond husks with the aim of exploring these byproducts as a source of bioactives to be used in nutraceutical and cosmetic formulations. METHODS: Methanol, ethanol and ethanol-water extracts of the almond husks were analysed by high-resolution liquid chromatography electrospray ionisation Orbitrap mass spectrometry (HR-LC-ESI-Orbitrap-MS) in negative ion mode. Tandem mass spectrometry (MS/MS) data were acquired by using the Data-Dependent Scan experiment, allowing the precursor ion to be selected as the most intense peak during LC-MS analyses. Nuclear magnetic resonance (NMR) experiments were performed on a Bruker DRX-600 spectrometer. Folin-Ciocalteu, DPPH• (2,2-diphenyl-1-picrylhydrazyl) and TEAC (Trolox equivalent antioxidant capacity) assays were employed to determine the total phenolic content and the radical scavenging activity of the extracts. RESULTS: The LC-MS/MS analysis of the methanol extract guided the isolation of phloroglucinol derivatives, flavonoids and terpenes. Eco-friendly extraction methods showed to be selective in extracting flavonoids while the comparison of the LC-MS profiles of the Italian cultivars Toritto and Avola showed significant differences, confirming how different growing conditions may influence the metabolome of a plant species. CONCLUSION: This study led to a deeper insight into the chemical constituents of almond husks and showed how the eco-friendly extraction resulted in an effective method to obtain extracts rich in antioxidant sources. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/30762260/HR_LC_ESI_Orbitrap_MS_based_metabolite_profiling_of_Prunus_dulcis_Mill___Italian_cultivars_Toritto_and_Avola__husks_and_evaluation_of_antioxidant_activity_ L2 - https://doi.org/10.1002/pca.2824 DB - PRIME DP - Unbound Medicine ER -