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The scolicidal activity of the essential oil obtained from the needles of Pinus nigra Arn. subsp. pallasiana (Lamb.) Holmboe on hydatid cyst.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

This study aimed to evaluate the in vitro activity of the essential oil of Pinus nigra subsp. pallasiana against protoscoleces of Echinococcus granulosus sensu lato. In traditional Turkish folk medicine, the essential oils and resin of P. nigra and P. sylvestris are used externally for wound healing. They are also used in cattle for treating worms and as boiling essence against cold and stomachache. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts.

MATERIALS AND METHODS

Three concentrations (10, 30, and 50 mg/mL) of the essential oil from P. nigra subsp. pallasiana cones were used for 10-60 min. The essential oil was applied to protoscoleces of hydatid cyst to determine the scolicidal activity of the essential oil. Viability of protoscoleces was observed by 0.1% eosin staining.

RESULTS

The rate of dead protoscoleces was found to be 2.27% in the control group, which was not treated with essential oil. The rate of dead protoscoleces increased to 61.69%, 69.39%, 75.98% and 71.76% respectively after, 10, 20, 30, and 60 min when the protoscoleces were exposed to the P. nigra subsp. pallasiana essential oil at 10 mg/mL concentration. When protoscoleces were exposed to the essential oil of P. nigra subsp. pallasiana for 10, 20, 30, and 60 min at 30 mg/mL concentration, the mortality rates of protoscoleces were determined as 3.82%, 91.33%, 94.67% and 96.74%, respectively. The mortality rate of 100% was determined at 50 mg/mL concentration after 60 min of exposure. According to statistical analysis, P. nigra subsp. pallasiana essential oil at all concentrations had significant scolicidal effects compared to the control group at all exposure times.

CONCLUSIONS

The essential oil of P. nigra subsp. pallasiana needles showed scolicidal activity and it could be a new scolicidal agent for using in hydatid cyst treatment.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Parasitology, 03200 Afyonkarahisar, Turkey.

    ,

    Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, Etiler 06330, Ankara, Turkey; Department of Pharmacognosy, Faculty of Pharmacy, Yüzüncü Yıl University, Tuşba 65080, Van, Turkey.

    ,

    Bartin University, Faculty of Forestry, Department of Forest Products Chemistry, 74100 Bartin, Turkey.

    Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, Etiler 06330, Ankara, Turkey. Electronic address: esrak@gazi.edu.tr.

    Source

    Journal of ethnopharmacology 235: 2019 May 10 pg 243-247

    MeSH

    Animals
    Anticestodal Agents
    Disease Models, Animal
    Dose-Response Relationship, Drug
    Echinococcosis, Hepatic
    Medicine, Traditional
    Oils, Volatile
    Pinus
    Sheep
    Time Factors
    Turkey

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30763692

    Citation

    Kozan, Esma, et al. "The Scolicidal Activity of the Essential Oil Obtained From the Needles of Pinus Nigra Arn. Subsp. Pallasiana (Lamb.) Holmboe On Hydatid Cyst." Journal of Ethnopharmacology, vol. 235, 2019, pp. 243-247.
    Kozan E, Ilhan M, Tümen I, et al. The scolicidal activity of the essential oil obtained from the needles of Pinus nigra Arn. subsp. pallasiana (Lamb.) Holmboe on hydatid cyst. J Ethnopharmacol. 2019;235:243-247.
    Kozan, E., Ilhan, M., Tümen, I., & Küpeli Akkol, E. (2019). The scolicidal activity of the essential oil obtained from the needles of Pinus nigra Arn. subsp. pallasiana (Lamb.) Holmboe on hydatid cyst. Journal of Ethnopharmacology, 235, pp. 243-247. doi:10.1016/j.jep.2019.02.018.
    Kozan E, et al. The Scolicidal Activity of the Essential Oil Obtained From the Needles of Pinus Nigra Arn. Subsp. Pallasiana (Lamb.) Holmboe On Hydatid Cyst. J Ethnopharmacol. 2019 May 10;235:243-247. PubMed PMID: 30763692.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The scolicidal activity of the essential oil obtained from the needles of Pinus nigra Arn. subsp. pallasiana (Lamb.) Holmboe on hydatid cyst. AU - Kozan,Esma, AU - Ilhan,Mert, AU - Tümen,Ibrahim, AU - Küpeli Akkol,Esra, Y1 - 2019/02/11/ PY - 2018/10/02/received PY - 2019/01/17/revised PY - 2019/02/10/accepted PY - 2019/2/15/pubmed PY - 2019/4/25/medline PY - 2019/2/15/entrez KW - Echinococcosis KW - Essential oil KW - Hydatid cyst KW - Pinaceae KW - Pinus nigra subsp. pallasiana SP - 243 EP - 247 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 235 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: This study aimed to evaluate the in vitro activity of the essential oil of Pinus nigra subsp. pallasiana against protoscoleces of Echinococcus granulosus sensu lato. In traditional Turkish folk medicine, the essential oils and resin of P. nigra and P. sylvestris are used externally for wound healing. They are also used in cattle for treating worms and as boiling essence against cold and stomachache. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. MATERIALS AND METHODS: Three concentrations (10, 30, and 50 mg/mL) of the essential oil from P. nigra subsp. pallasiana cones were used for 10-60 min. The essential oil was applied to protoscoleces of hydatid cyst to determine the scolicidal activity of the essential oil. Viability of protoscoleces was observed by 0.1% eosin staining. RESULTS: The rate of dead protoscoleces was found to be 2.27% in the control group, which was not treated with essential oil. The rate of dead protoscoleces increased to 61.69%, 69.39%, 75.98% and 71.76% respectively after, 10, 20, 30, and 60 min when the protoscoleces were exposed to the P. nigra subsp. pallasiana essential oil at 10 mg/mL concentration. When protoscoleces were exposed to the essential oil of P. nigra subsp. pallasiana for 10, 20, 30, and 60 min at 30 mg/mL concentration, the mortality rates of protoscoleces were determined as 3.82%, 91.33%, 94.67% and 96.74%, respectively. The mortality rate of 100% was determined at 50 mg/mL concentration after 60 min of exposure. According to statistical analysis, P. nigra subsp. pallasiana essential oil at all concentrations had significant scolicidal effects compared to the control group at all exposure times. CONCLUSIONS: The essential oil of P. nigra subsp. pallasiana needles showed scolicidal activity and it could be a new scolicidal agent for using in hydatid cyst treatment. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/30763692/The_scolicidal_activity_of_the_essential_oil_obtained_from_the_needles_of_Pinus_nigra_Arn__subsp__pallasiana__Lamb___Holmboe_on_hydatid_cyst_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(18)33636-5 DB - PRIME DP - Unbound Medicine ER -