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UDP-Glycosyltransferases are involved in imidacloprid resistance in the Asian citrus psyllid, Diaphorina citri (Hemiptera: Lividae).
Pestic Biochem Physiol. 2019 Feb; 154:23-31.PB

Abstract

UDP-glycosyltransferases (UGTs), as phase II detoxification enzymes, are widely distributed within living organisms and play vital roles in the biotransformation of endobiotics and xenobiotics in insects. Insects increase the expression of detoxification enzymes to cope with the stress of xenobiotics, including insecticides. However, the roles of UGTs in insecticide resistance are still seldom reported. In this study, two UGT inhibitors, namely, 5-nitrouracil and sulfinpyrazone, were found to synergistically increase the toxicity of imidacloprid in the resistant population of Diaphorina citri. Based on transcriptome data, a total of 17 putative UGTs were identified. Quantitative real-time PCR showed that fourteen of the 17 UGT genes were overexpressed in the resistant population relative to the susceptible population. Using RNA interference technology to knockdown six UGT genes, the results suggested that silencing the selected UGT375A1, UGT383A1, UGT383B1, and UGT384A1 genes dramatically increased the toxicity of imidacloprid in the resistant population. However, silencing the UGT362B1 and UGT379A1 genes did not result in a significant increase in the toxicity of imidacloprid in the resistant population. These findings revealed that some upregulated UGT genes were involved in imidacloprid resistance in D. citri. These results shed some light upon and further our understanding of the mechanisms of insecticide resistance in insects.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tian F
College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Wang Z
College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Li C
College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Liu J
College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Zeng X
College of Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: zengxn@scau.edu.cn.

MeSH

AnimalsFemaleGlucuronosyltransferaseHemipteraInsect ProteinsInsecticide ResistanceInsecticidesMaleNeonicotinoidsNitro CompoundsRNA, Messenger

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30765053

Citation

Tian, Fajun, et al. "UDP-Glycosyltransferases Are Involved in Imidacloprid Resistance in the Asian Citrus Psyllid, Diaphorina Citri (Hemiptera: Lividae)." Pesticide Biochemistry and Physiology, vol. 154, 2019, pp. 23-31.
Tian F, Wang Z, Li C, et al. UDP-Glycosyltransferases are involved in imidacloprid resistance in the Asian citrus psyllid, Diaphorina citri (Hemiptera: Lividae). Pestic Biochem Physiol. 2019;154:23-31.
Tian, F., Wang, Z., Li, C., Liu, J., & Zeng, X. (2019). UDP-Glycosyltransferases are involved in imidacloprid resistance in the Asian citrus psyllid, Diaphorina citri (Hemiptera: Lividae). Pesticide Biochemistry and Physiology, 154, 23-31. https://doi.org/10.1016/j.pestbp.2018.12.010
Tian F, et al. UDP-Glycosyltransferases Are Involved in Imidacloprid Resistance in the Asian Citrus Psyllid, Diaphorina Citri (Hemiptera: Lividae). Pestic Biochem Physiol. 2019;154:23-31. PubMed PMID: 30765053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - UDP-Glycosyltransferases are involved in imidacloprid resistance in the Asian citrus psyllid, Diaphorina citri (Hemiptera: Lividae). AU - Tian,Fajun, AU - Wang,Zhengbing, AU - Li,Chaofeng, AU - Liu,Jiali, AU - Zeng,Xinnian, Y1 - 2018/12/18/ PY - 2018/09/19/received PY - 2018/12/17/revised PY - 2018/12/17/accepted PY - 2019/2/16/entrez PY - 2019/2/16/pubmed PY - 2019/3/6/medline KW - Diaphorina citri KW - Imidacloprid KW - Insecticide resistance KW - RNAi KW - UDP-glycosyltransferases SP - 23 EP - 31 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 154 N2 - UDP-glycosyltransferases (UGTs), as phase II detoxification enzymes, are widely distributed within living organisms and play vital roles in the biotransformation of endobiotics and xenobiotics in insects. Insects increase the expression of detoxification enzymes to cope with the stress of xenobiotics, including insecticides. However, the roles of UGTs in insecticide resistance are still seldom reported. In this study, two UGT inhibitors, namely, 5-nitrouracil and sulfinpyrazone, were found to synergistically increase the toxicity of imidacloprid in the resistant population of Diaphorina citri. Based on transcriptome data, a total of 17 putative UGTs were identified. Quantitative real-time PCR showed that fourteen of the 17 UGT genes were overexpressed in the resistant population relative to the susceptible population. Using RNA interference technology to knockdown six UGT genes, the results suggested that silencing the selected UGT375A1, UGT383A1, UGT383B1, and UGT384A1 genes dramatically increased the toxicity of imidacloprid in the resistant population. However, silencing the UGT362B1 and UGT379A1 genes did not result in a significant increase in the toxicity of imidacloprid in the resistant population. These findings revealed that some upregulated UGT genes were involved in imidacloprid resistance in D. citri. These results shed some light upon and further our understanding of the mechanisms of insecticide resistance in insects. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/30765053/UDP_Glycosyltransferases_are_involved_in_imidacloprid_resistance_in_the_Asian_citrus_psyllid_Diaphorina_citri__Hemiptera:_Lividae__ DB - PRIME DP - Unbound Medicine ER -
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