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Analysis of Acetyl Fentanyl in Postmortem Specimens by Gas Chromatography-Mass Spectrometry (GC-MS): Method Validation and Case Report.
J Anal Toxicol. 2019 Jun 01; 43(5):392-398.JA

Abstract

In 2013, the Centers for Disease Control and Prevention released a warning regarding a new recreational drug, acetyl fentanyl. Acetyl fentanyl is a μ-opioid receptor agonist, and its pharmacological effects include euphoria, altered mood, miosis and central nervous system depression. The objective of this report was to develop a sensitive and specific method for the quantitation of acetyl fentanyl by gas chromatography-mass spectrometry in postmortem casework. Acetyl fentanyl was isolated from biological matrices using solid-phase extraction and acetyl fentanyl-13C6 was employed as an internal standard. The method was validated utilizing the Scientific Working Group for Forensic Toxicology's published method validation parameters, and the biological matrices used for analysis were postmortem blood and urine. In addition to the quantitation of acetyl fentanyl, a demographic study of cases obtained from the Rhode Island Office of State Medical Examiners and the University of Florida Health Pathology Laboratories-Forensic Toxicology Laboratory was performed to examine potential risk factors for acetyl fentanyl use. The results from this study found that the blood concentrations in these individuals ranged from 17 to 945 ng/mL. This suggests acetyl fentanyl is less potent than its prototype drug, fentanyl and requires an increased dose to achieve its desired effects. The demographic analysis indicated white males aged 21-40 years and individuals with a previous history of drug use have the highest risk for acetyl fentanyl abuse.

Authors+Show Affiliations

Miami-Dade County Medical Examiner Department, Toxicology Laboratory, 1851 NW 10th Avenue, Miami FL, USA.UF Health Pathology Laboratories-Forensic Toxicology Laboratory, Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, 4800 SW 35th Drive, Gainesville FL, USA.Office of the Chief Medical Examiner, 720 Albany Street, Boston MA, USA.State Health Laboratories, State of Rhode Island Department of Health, 50 Orms Street, Providence RI, USA.UF Health Pathology Laboratories-Forensic Toxicology Laboratory, Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, 4800 SW 35th Drive, Gainesville FL, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30767008

Citation

Finkelstein, Marissa J., et al. "Analysis of Acetyl Fentanyl in Postmortem Specimens By Gas Chromatography-Mass Spectrometry (GC-MS): Method Validation and Case Report." Journal of Analytical Toxicology, vol. 43, no. 5, 2019, pp. 392-398.
Finkelstein MJ, Chronister CW, Stanley C, et al. Analysis of Acetyl Fentanyl in Postmortem Specimens by Gas Chromatography-Mass Spectrometry (GC-MS): Method Validation and Case Report. J Anal Toxicol. 2019;43(5):392-398.
Finkelstein, M. J., Chronister, C. W., Stanley, C., Ogilvie, L. M., & Goldberger, B. A. (2019). Analysis of Acetyl Fentanyl in Postmortem Specimens by Gas Chromatography-Mass Spectrometry (GC-MS): Method Validation and Case Report. Journal of Analytical Toxicology, 43(5), 392-398. https://doi.org/10.1093/jat/bky108
Finkelstein MJ, et al. Analysis of Acetyl Fentanyl in Postmortem Specimens By Gas Chromatography-Mass Spectrometry (GC-MS): Method Validation and Case Report. J Anal Toxicol. 2019 Jun 1;43(5):392-398. PubMed PMID: 30767008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Acetyl Fentanyl in Postmortem Specimens by Gas Chromatography-Mass Spectrometry (GC-MS): Method Validation and Case Report. AU - Finkelstein,Marissa J, AU - Chronister,Chris W, AU - Stanley,Christina, AU - Ogilvie,Laurie M, AU - Goldberger,Bruce A, PY - 2018/06/08/received PY - 2018/12/17/revised PY - 2019/2/16/pubmed PY - 2020/1/8/medline PY - 2019/2/16/entrez KW - acetyl fentanyl. GC–MS KW - method validation KW - postmortem toxicology SP - 392 EP - 398 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 43 IS - 5 N2 - In 2013, the Centers for Disease Control and Prevention released a warning regarding a new recreational drug, acetyl fentanyl. Acetyl fentanyl is a μ-opioid receptor agonist, and its pharmacological effects include euphoria, altered mood, miosis and central nervous system depression. The objective of this report was to develop a sensitive and specific method for the quantitation of acetyl fentanyl by gas chromatography-mass spectrometry in postmortem casework. Acetyl fentanyl was isolated from biological matrices using solid-phase extraction and acetyl fentanyl-13C6 was employed as an internal standard. The method was validated utilizing the Scientific Working Group for Forensic Toxicology's published method validation parameters, and the biological matrices used for analysis were postmortem blood and urine. In addition to the quantitation of acetyl fentanyl, a demographic study of cases obtained from the Rhode Island Office of State Medical Examiners and the University of Florida Health Pathology Laboratories-Forensic Toxicology Laboratory was performed to examine potential risk factors for acetyl fentanyl use. The results from this study found that the blood concentrations in these individuals ranged from 17 to 945 ng/mL. This suggests acetyl fentanyl is less potent than its prototype drug, fentanyl and requires an increased dose to achieve its desired effects. The demographic analysis indicated white males aged 21-40 years and individuals with a previous history of drug use have the highest risk for acetyl fentanyl abuse. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/30767008/Analysis_of_Acetyl_Fentanyl_in_Postmortem_Specimens_by_Gas_Chromatography_Mass_Spectrometry__GC_MS_:_Method_Validation_and_Case_Report_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bky108 DB - PRIME DP - Unbound Medicine ER -