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Evaluation of biomarkers of kidney injury following 4% succinylated gelatin and 6% hydroxyethyl starch 130/0.4 administration in a canine hemorrhagic shock model.
J Vet Emerg Crit Care (San Antonio). 2019 Mar; 29(2):132-142.JV

Abstract

OBJECTIVE

To investigate the association between synthetic colloids and biomarkers of acute kidney injury (AKI) in dogs with hemorrhagic shock.

DESIGN

Experimental interventional study.

SETTING

University.

ANIMALS

Twenty-four healthy ex-racing Greyhounds.

INTERVENTIONS

Anesthetized Greyhounds subjected to hemorrhage for 60 min were resuscitated with 20 mL/kg of fresh whole blood (FWB), 6% hydroxyethyl starch (HES) 130/0.4, 4% succinylated gelatin (GELO), or 80 mL/kg of isotonic crystalloid (CRYST) over 20 min (n = 6 per treatment). Concentrations of biomarkers of AKI were measured at baseline, end of hemorrhage, and at 40 (T60), 100 (T120), and 160 (T180) min after fluid bolus. Biomarkers included neutrophil gelatinase-associated lipocalin in urine and serum (uNGAL; sNGAL), and urine cystatin C (uCYSC), kidney injury molecule-1 (uKIM), clusterin (uCLUST), osteopontin, gamma-glutamyl transferase, monocyte chemoattractant protein-1 (uMCP), interleukin-6, interleukin-8, protein (uPROT), hyaluronan, and F2 -isoprostanes. Renal histology was scored for tubular injury and microvesiculation. Biomarker fold-change from baseline was compared between groups using mixed effects models (Bonferroni-Holm corrected P<0.05). Frequencies of histology scores were compared by Fisher's exact test.

MEASUREMENTS AND MAIN RESULTS

In dogs treated with GELO, uNGAL fold-change was markedly greater compared with all other groups at T60, T120, and T180 (all P<0.001), and uCYSC was greater at T60 compared with CRYST (P<0.001), and at T120 and T180 compared with all other groups (all P<0.001). Smaller, albeit significant, between-group differences in uKIM, uCLUST, uMCP, and urine protein concentration were observed across the FWB, GELO, and HES groups, compared with CRYST. The GELO group more frequently had marked tubular microvesiculation than the other groups (P = 0.015) although tubular injury scores were comparable.

CONCLUSION

In dogs with hemorrhagic shock, GELO was associated with greater magnitude increases in urine biomarkers of AKI and more frequent marked tubular microvesiculation, compared with FWB, CRYST, and HES.

Authors+Show Affiliations

School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA.Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, WA, Australia. Emergency Medicine, University of Western Australia, WA, Australia.School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.Medical School, Royal Perth Hospital Unit, University of Western Australia, Perth, WA, Australia.Medical School, Royal Perth Hospital Unit, University of Western Australia, Perth, WA, Australia.School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia. Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, WA, Australia. Emergency Medicine, University of Western Australia, WA, Australia.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30767375

Citation

Boyd, Corrin J., et al. "Evaluation of Biomarkers of Kidney Injury Following 4% Succinylated Gelatin and 6% Hydroxyethyl Starch 130/0.4 Administration in a Canine Hemorrhagic Shock Model." Journal of Veterinary Emergency and Critical Care (San Antonio, Tex. : 2001), vol. 29, no. 2, 2019, pp. 132-142.
Boyd CJ, Claus MA, Raisis AL, et al. Evaluation of biomarkers of kidney injury following 4% succinylated gelatin and 6% hydroxyethyl starch 130/0.4 administration in a canine hemorrhagic shock model. J Vet Emerg Crit Care (San Antonio). 2019;29(2):132-142.
Boyd, C. J., Claus, M. A., Raisis, A. L., Cianciolo, R., Bosio, E., Hosgood, G., Nabity, M., Mori, T., Barden, A., Sharp, C. R., & Smart, L. (2019). Evaluation of biomarkers of kidney injury following 4% succinylated gelatin and 6% hydroxyethyl starch 130/0.4 administration in a canine hemorrhagic shock model. Journal of Veterinary Emergency and Critical Care (San Antonio, Tex. : 2001), 29(2), 132-142. https://doi.org/10.1111/vec.12814
Boyd CJ, et al. Evaluation of Biomarkers of Kidney Injury Following 4% Succinylated Gelatin and 6% Hydroxyethyl Starch 130/0.4 Administration in a Canine Hemorrhagic Shock Model. J Vet Emerg Crit Care (San Antonio). 2019;29(2):132-142. PubMed PMID: 30767375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of biomarkers of kidney injury following 4% succinylated gelatin and 6% hydroxyethyl starch 130/0.4 administration in a canine hemorrhagic shock model. AU - Boyd,Corrin J, AU - Claus,Melissa A, AU - Raisis,Anthea L, AU - Cianciolo,Rachel, AU - Bosio,Erika, AU - Hosgood,Giselle, AU - Nabity,Mary, AU - Mori,Trevor, AU - Barden,Anne, AU - Sharp,Claire R, AU - Smart,Lisa, Y1 - 2019/02/14/ PY - 2018/10/30/received PY - 2018/12/20/revised PY - 2019/01/24/accepted PY - 2019/2/16/pubmed PY - 2019/5/6/medline PY - 2019/2/16/entrez KW - cystatin C KW - fluid therapy KW - neutrophil gelatinase-associated lipocalin KW - synthetic colloids KW - urinary biomarkers SP - 132 EP - 142 JF - Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001) JO - J Vet Emerg Crit Care (San Antonio) VL - 29 IS - 2 N2 - OBJECTIVE: To investigate the association between synthetic colloids and biomarkers of acute kidney injury (AKI) in dogs with hemorrhagic shock. DESIGN: Experimental interventional study. SETTING: University. ANIMALS: Twenty-four healthy ex-racing Greyhounds. INTERVENTIONS: Anesthetized Greyhounds subjected to hemorrhage for 60 min were resuscitated with 20 mL/kg of fresh whole blood (FWB), 6% hydroxyethyl starch (HES) 130/0.4, 4% succinylated gelatin (GELO), or 80 mL/kg of isotonic crystalloid (CRYST) over 20 min (n = 6 per treatment). Concentrations of biomarkers of AKI were measured at baseline, end of hemorrhage, and at 40 (T60), 100 (T120), and 160 (T180) min after fluid bolus. Biomarkers included neutrophil gelatinase-associated lipocalin in urine and serum (uNGAL; sNGAL), and urine cystatin C (uCYSC), kidney injury molecule-1 (uKIM), clusterin (uCLUST), osteopontin, gamma-glutamyl transferase, monocyte chemoattractant protein-1 (uMCP), interleukin-6, interleukin-8, protein (uPROT), hyaluronan, and F2 -isoprostanes. Renal histology was scored for tubular injury and microvesiculation. Biomarker fold-change from baseline was compared between groups using mixed effects models (Bonferroni-Holm corrected P<0.05). Frequencies of histology scores were compared by Fisher's exact test. MEASUREMENTS AND MAIN RESULTS: In dogs treated with GELO, uNGAL fold-change was markedly greater compared with all other groups at T60, T120, and T180 (all P<0.001), and uCYSC was greater at T60 compared with CRYST (P<0.001), and at T120 and T180 compared with all other groups (all P<0.001). Smaller, albeit significant, between-group differences in uKIM, uCLUST, uMCP, and urine protein concentration were observed across the FWB, GELO, and HES groups, compared with CRYST. The GELO group more frequently had marked tubular microvesiculation than the other groups (P = 0.015) although tubular injury scores were comparable. CONCLUSION: In dogs with hemorrhagic shock, GELO was associated with greater magnitude increases in urine biomarkers of AKI and more frequent marked tubular microvesiculation, compared with FWB, CRYST, and HES. SN - 1476-4431 UR - https://www.unboundmedicine.com/medline/citation/30767375/Evaluation_of_biomarkers_of_kidney_injury_following_4_succinylated_gelatin_and_6_hydroxyethyl_starch_130/0_4_administration_in_a_canine_hemorrhagic_shock_model_ L2 - https://doi.org/10.1111/vec.12814 DB - PRIME DP - Unbound Medicine ER -