Tags

Type your tag names separated by a space and hit enter

Impact of Bioconversion of Gallated Catechins and Flavonol Glycosides on Bioaccessibility and Intestinal Cellular Uptake of Catechins.
J Agric Food Chem. 2019 Feb 27; 67(8):2331-2339.JA

Abstract

Two bioconversions were applied to green tea extracts (GTE) and flavonol glycoside rich fraction (FVNg) derived from insoluble green tea extract by tannase and cellulase treatment in order to obtain gallated catechins (EnzGTE) and flavonol aglycone rich fraction (FVNa), respectively. The bioaccessibility of epicatechins from GTE increased with the addition of FVNg, FVNa, and flavonol aglycone rich fraction of commercial production (FVNap). Epigallocatechin-gallate (EGCG) and epicatechin-gallate (ECG) were highly recovered 4- and 125-fold, respectively, by adding FVNap. They were mostly affected by the radical scavenging activity provided from FVNap, showing remarkable 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (10769.3 μg/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (8341.5 μg/g) values. The intestinal cellular uptake of epicatechins in GTE increased with the FVNap addition as follows: EGCG (332.46 ± 136.18%) > ECG (273.92 ± 97.92%) > epicatechin (EC) (150.22 ± 12.59%) > epigallocatechin (EGC) (131.21 ± 8.51%). EnzGTE and EnzGTE + FVNa were revealed to have a significant downregulation on the expression of P-glycoprotein (P-gp), up to 0.06- and 0.6-fold, respectively. The gene expression of multidrug resistance associated proteins 2 (MRP2) was reduced in EnzGTE + FVNap. The results suggest that coconsumption GTE or EnzGTE with GTE-derived flavonols could improve the bioavailability of epicatechins.

Authors+Show Affiliations

Department of Food Science and Biotechnology , Sejong University , 98 Gunja-dong , Gwangjin-gu, Seoul 143-747 , Republic of Korea.Vital Beautie Research Institute , AmorePacific R&D Center , Yongin 17074 , Republic of Korea.Department of Food Science and Biotechnology , Sejong University , 98 Gunja-dong , Gwangjin-gu, Seoul 143-747 , Republic of Korea.Department of Food Science and Biotechnology , Kyung Hee University , Yongin 17104 , Republic of Korea.Department of Food Science and Biotechnology , Sejong University , 98 Gunja-dong , Gwangjin-gu, Seoul 143-747 , Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30767525

Citation

Choi, Eun-Hye, et al. "Impact of Bioconversion of Gallated Catechins and Flavonol Glycosides On Bioaccessibility and Intestinal Cellular Uptake of Catechins." Journal of Agricultural and Food Chemistry, vol. 67, no. 8, 2019, pp. 2331-2339.
Choi EH, Rha CS, Balusamy SR, et al. Impact of Bioconversion of Gallated Catechins and Flavonol Glycosides on Bioaccessibility and Intestinal Cellular Uptake of Catechins. J Agric Food Chem. 2019;67(8):2331-2339.
Choi, E. H., Rha, C. S., Balusamy, S. R., Kim, D. O., & Shim, S. M. (2019). Impact of Bioconversion of Gallated Catechins and Flavonol Glycosides on Bioaccessibility and Intestinal Cellular Uptake of Catechins. Journal of Agricultural and Food Chemistry, 67(8), 2331-2339. https://doi.org/10.1021/acs.jafc.8b05733
Choi EH, et al. Impact of Bioconversion of Gallated Catechins and Flavonol Glycosides On Bioaccessibility and Intestinal Cellular Uptake of Catechins. J Agric Food Chem. 2019 Feb 27;67(8):2331-2339. PubMed PMID: 30767525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of Bioconversion of Gallated Catechins and Flavonol Glycosides on Bioaccessibility and Intestinal Cellular Uptake of Catechins. AU - Choi,Eun-Hye, AU - Rha,Chan-Su, AU - Balusamy,Sri Renukadevi, AU - Kim,Dae-Ok, AU - Shim,Soon-Mi, Y1 - 2019/02/15/ PY - 2019/2/16/pubmed PY - 2019/3/9/medline PY - 2019/2/16/entrez KW - bioaccessibility KW - catechin KW - green tea derived flavonols KW - intestinal absorption KW - tannase treated green tea SP - 2331 EP - 2339 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 67 IS - 8 N2 - Two bioconversions were applied to green tea extracts (GTE) and flavonol glycoside rich fraction (FVNg) derived from insoluble green tea extract by tannase and cellulase treatment in order to obtain gallated catechins (EnzGTE) and flavonol aglycone rich fraction (FVNa), respectively. The bioaccessibility of epicatechins from GTE increased with the addition of FVNg, FVNa, and flavonol aglycone rich fraction of commercial production (FVNap). Epigallocatechin-gallate (EGCG) and epicatechin-gallate (ECG) were highly recovered 4- and 125-fold, respectively, by adding FVNap. They were mostly affected by the radical scavenging activity provided from FVNap, showing remarkable 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (10769.3 μg/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (8341.5 μg/g) values. The intestinal cellular uptake of epicatechins in GTE increased with the FVNap addition as follows: EGCG (332.46 ± 136.18%) > ECG (273.92 ± 97.92%) > epicatechin (EC) (150.22 ± 12.59%) > epigallocatechin (EGC) (131.21 ± 8.51%). EnzGTE and EnzGTE + FVNa were revealed to have a significant downregulation on the expression of P-glycoprotein (P-gp), up to 0.06- and 0.6-fold, respectively. The gene expression of multidrug resistance associated proteins 2 (MRP2) was reduced in EnzGTE + FVNap. The results suggest that coconsumption GTE or EnzGTE with GTE-derived flavonols could improve the bioavailability of epicatechins. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/30767525/Impact_of_Bioconversion_of_Gallated_Catechins_and_Flavonol_Glycosides_on_Bioaccessibility_and_Intestinal_Cellular_Uptake_of_Catechins_ L2 - https://doi.org/10.1021/acs.jafc.8b05733 DB - PRIME DP - Unbound Medicine ER -