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Aegle marmelos leaf extract ameliorates the cognitive impairment and oxidative stress induced by intracerebroventricular streptozotocin in male rats.
Life Sci. 2019 Mar 15; 221:196-203.LS

Abstract

AIMS

Aegle marmelos (L.) Correa (A. marmelos) has been used in Ayurvedic medicine as a brain tonic however its neuroprotective effect against streptozotocin (STZ) induced cognitive impairment and oxidative stress has not been reported yet in vivo. Therefore, the present study was attempted to investigate the neuroprotective potential of ethanolic extract of A. marmelos leaves (AME) on STZ induced memory impairment in male rats.

MAIN METHODS

Albino Wistar rats were pre-treated orally with AME at the doses 200 and 400 mg/kg for two weeks, followed by intracerebroventricular (i.c.v.) injection of STZ (3 mg/kg) on day 1 and 3. Two weeks after STZ administration, behavioural parameters were monitored using Morris water maze task. Biochemical and histopathological studies were carried out after three weeks of STZ administration. The levels of oxidative stress markers (malondialdehyde (MDA), glutathione, nitrite, catalase) neuroinflammatory mediators; tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and acetylcholinesterase (AChE) activity were estimated in hippocampus of rat brain. Donepezil (5 mg/kg) was taken as a standard drug.

KEY FINDINGS

The levels of MDA, nitrite, TNF-α and IL-6 were significantly increased while glutathione levels were significantly decreased in hippocampus of STZ-treated rats. Further, a significant decrease in the activity of catalase and increase in AChE activity was observed indicating cholinergic hypofunction and neuronal damage in STZ-treated animals. All these alterations were significantly ameliorated by AME in a dose dependent manner.

SIGNIFICANCE

The neuroprotective potential of A. marmelos against STZ induced oxidative stress and cognitive deficit in rats indicates its therapeutic value in Alzheimer's disease (AD).

Authors+Show Affiliations

IKG Punjab Technical University, Jalandhar, Kapurthala 144603, Punjab, India; Jan Nayak Ch. Devi Lal Memorial College of Pharmacy, Sirsa 125055, Haryana, India.IKG Punjab Technical University, Jalandhar, Kapurthala 144603, Punjab, India; Jan Nayak Ch. Devi Lal Memorial College of Pharmacy, Sirsa 125055, Haryana, India.Chandigarh College of Pharmacy, Landran, Mohali 140307, Punjab, India.Amity Institute of Pharmacy, Amity University, Sector-125, Noida 201313, India.Amity Institute of Molecular Medicine & Stem Cell Research, Amity University, Sector-125, Noida 201313, India. Electronic address: dpandita@amity.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30771313

Citation

Raheja, Shikha, et al. "Aegle Marmelos Leaf Extract Ameliorates the Cognitive Impairment and Oxidative Stress Induced By Intracerebroventricular Streptozotocin in Male Rats." Life Sciences, vol. 221, 2019, pp. 196-203.
Raheja S, Girdhar A, Kamboj A, et al. Aegle marmelos leaf extract ameliorates the cognitive impairment and oxidative stress induced by intracerebroventricular streptozotocin in male rats. Life Sci. 2019;221:196-203.
Raheja, S., Girdhar, A., Kamboj, A., Lather, V., & Pandita, D. (2019). Aegle marmelos leaf extract ameliorates the cognitive impairment and oxidative stress induced by intracerebroventricular streptozotocin in male rats. Life Sciences, 221, 196-203. https://doi.org/10.1016/j.lfs.2019.02.032
Raheja S, et al. Aegle Marmelos Leaf Extract Ameliorates the Cognitive Impairment and Oxidative Stress Induced By Intracerebroventricular Streptozotocin in Male Rats. Life Sci. 2019 Mar 15;221:196-203. PubMed PMID: 30771313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aegle marmelos leaf extract ameliorates the cognitive impairment and oxidative stress induced by intracerebroventricular streptozotocin in male rats. AU - Raheja,Shikha, AU - Girdhar,Amit, AU - Kamboj,Anjoo, AU - Lather,Viney, AU - Pandita,Deepti, Y1 - 2019/02/14/ PY - 2018/10/16/received PY - 2019/02/06/revised PY - 2019/02/13/accepted PY - 2019/2/17/pubmed PY - 2019/4/4/medline PY - 2019/2/17/entrez KW - A. marmelos KW - Alzheimer's disease KW - Memory impairment KW - Oxidative stress KW - Streptozotocin SP - 196 EP - 203 JF - Life sciences JO - Life Sci VL - 221 N2 - AIMS: Aegle marmelos (L.) Correa (A. marmelos) has been used in Ayurvedic medicine as a brain tonic however its neuroprotective effect against streptozotocin (STZ) induced cognitive impairment and oxidative stress has not been reported yet in vivo. Therefore, the present study was attempted to investigate the neuroprotective potential of ethanolic extract of A. marmelos leaves (AME) on STZ induced memory impairment in male rats. MAIN METHODS: Albino Wistar rats were pre-treated orally with AME at the doses 200 and 400 mg/kg for two weeks, followed by intracerebroventricular (i.c.v.) injection of STZ (3 mg/kg) on day 1 and 3. Two weeks after STZ administration, behavioural parameters were monitored using Morris water maze task. Biochemical and histopathological studies were carried out after three weeks of STZ administration. The levels of oxidative stress markers (malondialdehyde (MDA), glutathione, nitrite, catalase) neuroinflammatory mediators; tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and acetylcholinesterase (AChE) activity were estimated in hippocampus of rat brain. Donepezil (5 mg/kg) was taken as a standard drug. KEY FINDINGS: The levels of MDA, nitrite, TNF-α and IL-6 were significantly increased while glutathione levels were significantly decreased in hippocampus of STZ-treated rats. Further, a significant decrease in the activity of catalase and increase in AChE activity was observed indicating cholinergic hypofunction and neuronal damage in STZ-treated animals. All these alterations were significantly ameliorated by AME in a dose dependent manner. SIGNIFICANCE: The neuroprotective potential of A. marmelos against STZ induced oxidative stress and cognitive deficit in rats indicates its therapeutic value in Alzheimer's disease (AD). SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/30771313/Aegle_marmelos_leaf_extract_ameliorates_the_cognitive_impairment_and_oxidative_stress_induced_by_intracerebroventricular_streptozotocin_in_male_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(19)30122-5 DB - PRIME DP - Unbound Medicine ER -