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Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress.
Ecotoxicol Environ Saf. 2019 May 30; 173:214-224.EE

Abstract

The enrichment of Cadmium in vegetables is threatening human health. The study aimed to screen Cd low-enriched leafy vegetables and explore whether antioxidative enzymes and heavy metal chelators are synergistic defensive mechanisms. In this paper, the Cd accumulation and translocation of garland chrysanthemum (Chrysanthemum coronarium L.), spinach (Spinacia oleracea L.), and lettuce (Lactuca sativa L.) were examined by soil pot culture and hydroponic experiments. The responses of oxidative stress markers, antioxidative enzymes activity, and thiol pool (cysteine, γ-glutamylcysteine, glutathione, and phytochelatins) content to Cd stress were assayed. The results showed that Garland chrysanthemum was Cd low-uptake species. The soil Cd safety thresholds for spinach, lettuce, and garland chrysanthemum were 0.41, 0.49, and 9.10 mg kg-1, respectively. The order of root phytochelatins (PCs) concentration was consistent with that of plant tolerance index (TI): garland chrysanthemum > spinach > lettuce. While the order of the ratio of shoot Cd to root Cd (SR ratio) was exactly the opposite of that of TI. In lettuce root, activity of superoxide dismutase, peroxidase, and catalase decreased significantly under Cd stress. Nevertheless those parameters in the roots of spinach and lettuce maintained steady, or even enhanced. In conclusion, the Cd translocation and partition in plant, antioxidative defense, and PCs homeostasis played an important role in the Cd tolerance of vegetables.

Authors+Show Affiliations

College of Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.College of Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.College of Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: linquanw@nwsuaf.edu.cn.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: zhchju@nwsuaf.edu.cn.Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30772711

Citation

Meng, Yuan, et al. "Antioxidative Enzymes Activity and Thiol Metabolism in Three Leafy Vegetables Under Cd Stress." Ecotoxicology and Environmental Safety, vol. 173, 2019, pp. 214-224.
Meng Y, Zhang L, Wang L, et al. Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress. Ecotoxicol Environ Saf. 2019;173:214-224.
Meng, Y., Zhang, L., Wang, L., Zhou, C., Shangguan, Y., & Yang, Y. (2019). Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress. Ecotoxicology and Environmental Safety, 173, 214-224. https://doi.org/10.1016/j.ecoenv.2019.02.026
Meng Y, et al. Antioxidative Enzymes Activity and Thiol Metabolism in Three Leafy Vegetables Under Cd Stress. Ecotoxicol Environ Saf. 2019 May 30;173:214-224. PubMed PMID: 30772711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress. AU - Meng,Yuan, AU - Zhang,Liang, AU - Wang,Linquan, AU - Zhou,Chunju, AU - Shangguan,Yuxian, AU - Yang,Yang, Y1 - 2019/02/14/ PY - 2018/12/22/received PY - 2019/02/05/revised PY - 2019/02/07/accepted PY - 2019/2/18/pubmed PY - 2019/4/17/medline PY - 2019/2/18/entrez KW - GSH KW - Oxidative stress markers KW - PCs KW - POD KW - SOD KW - SR ratio SP - 214 EP - 224 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 173 N2 - The enrichment of Cadmium in vegetables is threatening human health. The study aimed to screen Cd low-enriched leafy vegetables and explore whether antioxidative enzymes and heavy metal chelators are synergistic defensive mechanisms. In this paper, the Cd accumulation and translocation of garland chrysanthemum (Chrysanthemum coronarium L.), spinach (Spinacia oleracea L.), and lettuce (Lactuca sativa L.) were examined by soil pot culture and hydroponic experiments. The responses of oxidative stress markers, antioxidative enzymes activity, and thiol pool (cysteine, γ-glutamylcysteine, glutathione, and phytochelatins) content to Cd stress were assayed. The results showed that Garland chrysanthemum was Cd low-uptake species. The soil Cd safety thresholds for spinach, lettuce, and garland chrysanthemum were 0.41, 0.49, and 9.10 mg kg-1, respectively. The order of root phytochelatins (PCs) concentration was consistent with that of plant tolerance index (TI): garland chrysanthemum > spinach > lettuce. While the order of the ratio of shoot Cd to root Cd (SR ratio) was exactly the opposite of that of TI. In lettuce root, activity of superoxide dismutase, peroxidase, and catalase decreased significantly under Cd stress. Nevertheless those parameters in the roots of spinach and lettuce maintained steady, or even enhanced. In conclusion, the Cd translocation and partition in plant, antioxidative defense, and PCs homeostasis played an important role in the Cd tolerance of vegetables. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/30772711/Antioxidative_enzymes_activity_and_thiol_metabolism_in_three_leafy_vegetables_under_Cd_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(19)30176-9 DB - PRIME DP - Unbound Medicine ER -