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Prevalence and antimicrobial resistance of Shigella species isolated from diarrheal patients in Ahvaz, southwest Iran.
Infect Drug Resist 2019; 12:249-253ID

Abstract

Introduction

Shigellosis is a significant global human health problem, and Shigella is in charge of almost 165 million cases of this disease annually, of whom 163 million cases are in developing countries and 1.5 million cases are in developed countries. The main aims of the current survey were to identify Shigella spp. isolated from diarrheal patients by conventional biochemical tests, determine the antimicrobial susceptibility profiles by disk diffusion method, and detect the ipaH gene using the PCR assay.

Methods

The bacterial isolates were identified as Shigella spp. by microbiological tests and were serogrouped by the slide agglutination test. Antimicrobial susceptibility testing was performed using the disk diffusion method. PCR was performed to detect the ipaH gene.

Results

The Shigella strains were isolated from 522 patients with various diarrhea, including bloody diarrhea (3%), mucoid plus bloody diarrhea (1.9%), mucoid diarrhea (3.2%), and watery diarrhea (3.2%). Overall, 69 (13.2%) isolates were positive for Shigella spp., of which 34 (49.3%) serotypes were identified as Shigella flexneri, 22 (31.9%) serotypes were identified as Shigella sonnei, 9 (13%) serotypes were identified as Shigella boydii, and 4 (5.8%) serotypes were identified as Shigella dysenteriae. Antibiotic susceptibility tests revealed that the highest resistance percentage was related to ampicillin (82%) and trimethoprim-sulfamethoxazole (77%), and ciprofloxacin and ceftriaxone were the best antibiotics against Shigella isolates.

Conclusion

We concluded that Shigella spp. can be considered as an etiological agent of diarrhea in southwest Iran. Since the drug resistance pattern of Shigella differs geographically and over time within a country, continuous and regular surveillance program is necessary.

Authors+Show Affiliations

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences,Tehran, Iran. Student Research Committee, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.Abadan School of Medical Sciences, Abadan, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Saeed.Khoshnood22@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30774392

Citation

Sheikh, Ahmad Farajzadeh, et al. "Prevalence and Antimicrobial Resistance of Shigella Species Isolated From Diarrheal Patients in Ahvaz, Southwest Iran." Infection and Drug Resistance, vol. 12, 2019, pp. 249-253.
Sheikh AF, Moosavian M, Abdi M, et al. Prevalence and antimicrobial resistance of Shigella species isolated from diarrheal patients in Ahvaz, southwest Iran. Infect Drug Resist. 2019;12:249-253.
Sheikh, A. F., Moosavian, M., Abdi, M., Heidary, M., Shahi, F., Jomehzadeh, N., ... Khoshnood, S. (2019). Prevalence and antimicrobial resistance of Shigella species isolated from diarrheal patients in Ahvaz, southwest Iran. Infection and Drug Resistance, 12, pp. 249-253. doi:10.2147/IDR.S187861.
Sheikh AF, et al. Prevalence and Antimicrobial Resistance of Shigella Species Isolated From Diarrheal Patients in Ahvaz, Southwest Iran. Infect Drug Resist. 2019;12:249-253. PubMed PMID: 30774392.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and antimicrobial resistance of Shigella species isolated from diarrheal patients in Ahvaz, southwest Iran. AU - Sheikh,Ahmad Farajzadeh, AU - Moosavian,Mojtaba, AU - Abdi,Mahtab, AU - Heidary,Mohsen, AU - Shahi,Fatemeh, AU - Jomehzadeh,Nabi, AU - Seyed-Mohammadi,Sakineh, AU - Saki,Morteza, AU - Khoshnood,Saeed, Y1 - 2019/01/22/ PY - 2019/2/19/entrez PY - 2019/2/19/pubmed PY - 2019/2/19/medline KW - Iran KW - Shigella KW - diarrhea KW - resistance SP - 249 EP - 253 JF - Infection and drug resistance JO - Infect Drug Resist VL - 12 N2 - Introduction: Shigellosis is a significant global human health problem, and Shigella is in charge of almost 165 million cases of this disease annually, of whom 163 million cases are in developing countries and 1.5 million cases are in developed countries. The main aims of the current survey were to identify Shigella spp. isolated from diarrheal patients by conventional biochemical tests, determine the antimicrobial susceptibility profiles by disk diffusion method, and detect the ipaH gene using the PCR assay. Methods: The bacterial isolates were identified as Shigella spp. by microbiological tests and were serogrouped by the slide agglutination test. Antimicrobial susceptibility testing was performed using the disk diffusion method. PCR was performed to detect the ipaH gene. Results: The Shigella strains were isolated from 522 patients with various diarrhea, including bloody diarrhea (3%), mucoid plus bloody diarrhea (1.9%), mucoid diarrhea (3.2%), and watery diarrhea (3.2%). Overall, 69 (13.2%) isolates were positive for Shigella spp., of which 34 (49.3%) serotypes were identified as Shigella flexneri, 22 (31.9%) serotypes were identified as Shigella sonnei, 9 (13%) serotypes were identified as Shigella boydii, and 4 (5.8%) serotypes were identified as Shigella dysenteriae. Antibiotic susceptibility tests revealed that the highest resistance percentage was related to ampicillin (82%) and trimethoprim-sulfamethoxazole (77%), and ciprofloxacin and ceftriaxone were the best antibiotics against Shigella isolates. Conclusion: We concluded that Shigella spp. can be considered as an etiological agent of diarrhea in southwest Iran. Since the drug resistance pattern of Shigella differs geographically and over time within a country, continuous and regular surveillance program is necessary. SN - 1178-6973 UR - https://www.unboundmedicine.com/medline/citation/30774392/Prevalence_and_antimicrobial_resistance_of_Shigella_species_isolated_from_diarrheal_patients_in_Ahvaz_southwest_Iran_ L2 - https://dx.doi.org/10.2147/IDR.S187861 DB - PRIME DP - Unbound Medicine ER -