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First Report of Soybean Rust Caused by Phakopsora pachyrhizi in Ghana.
Plant Dis 2007; 91(8):1057PD

Abstract

Nigeria is the only country in West Africa where soybean rust, caused by Phakopsora pachyrhizi, has been officially reported (1). During a disease survey in Ghana during October 2006, soybean (Glycine max) leaves with rust symptoms (tan, angular lesions with erumpent sori exuding urediniospores) were observed in 11 fields in the following districts: Kassena Nankana in the Upper East Region; East Gonja, Central Gonja, and Tolon-Kumbungu in the Northern Region; and Ejisu-Juabeng in the Ashanti Region. Disease incidence in these fields ranged from 50 to 100% and disease severity ranged between 3 and 40% of the leaf area on infected plants. Urediniospores were hyaline, minutely echinulate, and 23 to 31 × 14 to 18 μm. Within a week of collection, leaf samples were sent to the USDA-ARS Foreign Disease-Weed Science Research Unit for verification of pathogen identity. DNA was extracted from leaf pieces containing sori with the Qiagen DNeasy Plant Mini kit (Valencia, CA), and all 11 field samples amplified in a real-time fluorescent PCR with the P. pachyrhizi-specific primers Ppm1 and Ppa2 (2). Sequence alignment of the internal transcribed spacer (ITS) region 2 further confirmed the identification as P. pachyrhizi (2). Infected leaves from three fields were separately washed in sterile water to collect urediniospores that were used to separately inoculate three detached leaves (for each isolate) of susceptible cultivar TGx 1485-1D (3). The abaxial surface of detached leaves was sprayed with 400 μl of spore suspension (1 × 106 spores per ml). A single leaf piece was placed in a 9-cm-diameter petri dish with adaxial side appressed on 1% technical agar amended with 10 μg/ml of kinetin. Lactic acid (1.5 ml/liter) and benomyl (12.5 mg/liter) were added to the agar medium to inhibit growth of saprophytic fungi and bacteria. Petri dishes were incubated at 20°C with a 12-h light/12-h dark cycle. Lesions on inoculated leaves developed 5 to 6 days after inoculation (DAI), and pustules (105 to 120 μm) formed 7 to 8 DAI and erupted 3 days later exuding columns of urediniospores similar in size to the initially collected isolates. Inoculating another set of detached leaves with a spore suspension (1 × 106 spores per ml) from the first set of detached leaves resulted in typical rust symptoms. The PCR assay, alignment of ITS region 2, morphological characters of the isolates, and pathogenicity tests demonstrate that P. pachyrhizi occurs in Ghana. To our knowledge, this is the first report of P. pachyrhizi in Ghana. References: (1) O. A. Akinsanmi et al. Plant Dis. 85:97, 2001. (2) R. D. Frederick et al. Phytopathology 92:217, 2002. (3) M. Twizeyimana et al. Online publication. http://www.plantmanagementnetwork.org/ infocenter/topic/soybeanrust/2006/posters/41.asp. Plant Management Network, 2006.

Authors+Show Affiliations

International Institute of Tropical Agriculture (IITA), PMB 5320, Ibadan, Nigeria.International Institute of Tropical Agriculture (IITA), PMB 5320, Ibadan, Nigeria.International Institute of Tropical Agriculture (IITA), PMB 5320, Ibadan, Nigeria.Crop Research Institute, Kumasi, Ghana.USDA-ARS Foreign Disease-Weed Science Research Unit, 1301 Ditto Ave., Fort Detrick, MD 21702.USDA-ARS Foreign Disease-Weed Science Research Unit, 1301 Ditto Ave., Fort Detrick, MD 21702.USDA-ARS Foreign Disease-Weed Science Research Unit, 1301 Ditto Ave., Fort Detrick, MD 21702.USDA-ARS and Department of Crop Sciences, University of Illinois, Urbana.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30780460

Citation

Bandyopadhyay, R, et al. "First Report of Soybean Rust Caused By Phakopsora Pachyrhizi in Ghana." Plant Disease, vol. 91, no. 8, 2007, p. 1057.
Bandyopadhyay R, Ojiambo PS, Twizeyimana M, et al. First Report of Soybean Rust Caused by Phakopsora pachyrhizi in Ghana. Plant Dis. 2007;91(8):1057.
Bandyopadhyay, R., Ojiambo, P. S., Twizeyimana, M., Asafo-Adjei, B., Frederick, R. D., Pedley, K. F., ... Hartman, G. L. (2007). First Report of Soybean Rust Caused by Phakopsora pachyrhizi in Ghana. Plant Disease, 91(8), p. 1057. doi:10.1094/PDIS-91-8-1057B.
Bandyopadhyay R, et al. First Report of Soybean Rust Caused By Phakopsora Pachyrhizi in Ghana. Plant Dis. 2007;91(8):1057. PubMed PMID: 30780460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First Report of Soybean Rust Caused by Phakopsora pachyrhizi in Ghana. AU - Bandyopadhyay,R, AU - Ojiambo,P S, AU - Twizeyimana,M, AU - Asafo-Adjei,B, AU - Frederick,R D, AU - Pedley,K F, AU - Stone,C L, AU - Hartman,G L, PY - 2019/2/21/entrez PY - 2007/8/1/pubmed PY - 2007/8/1/medline SP - 1057 EP - 1057 JF - Plant disease JO - Plant Dis. VL - 91 IS - 8 N2 - Nigeria is the only country in West Africa where soybean rust, caused by Phakopsora pachyrhizi, has been officially reported (1). During a disease survey in Ghana during October 2006, soybean (Glycine max) leaves with rust symptoms (tan, angular lesions with erumpent sori exuding urediniospores) were observed in 11 fields in the following districts: Kassena Nankana in the Upper East Region; East Gonja, Central Gonja, and Tolon-Kumbungu in the Northern Region; and Ejisu-Juabeng in the Ashanti Region. Disease incidence in these fields ranged from 50 to 100% and disease severity ranged between 3 and 40% of the leaf area on infected plants. Urediniospores were hyaline, minutely echinulate, and 23 to 31 × 14 to 18 μm. Within a week of collection, leaf samples were sent to the USDA-ARS Foreign Disease-Weed Science Research Unit for verification of pathogen identity. DNA was extracted from leaf pieces containing sori with the Qiagen DNeasy Plant Mini kit (Valencia, CA), and all 11 field samples amplified in a real-time fluorescent PCR with the P. pachyrhizi-specific primers Ppm1 and Ppa2 (2). Sequence alignment of the internal transcribed spacer (ITS) region 2 further confirmed the identification as P. pachyrhizi (2). Infected leaves from three fields were separately washed in sterile water to collect urediniospores that were used to separately inoculate three detached leaves (for each isolate) of susceptible cultivar TGx 1485-1D (3). The abaxial surface of detached leaves was sprayed with 400 μl of spore suspension (1 × 106 spores per ml). A single leaf piece was placed in a 9-cm-diameter petri dish with adaxial side appressed on 1% technical agar amended with 10 μg/ml of kinetin. Lactic acid (1.5 ml/liter) and benomyl (12.5 mg/liter) were added to the agar medium to inhibit growth of saprophytic fungi and bacteria. Petri dishes were incubated at 20°C with a 12-h light/12-h dark cycle. Lesions on inoculated leaves developed 5 to 6 days after inoculation (DAI), and pustules (105 to 120 μm) formed 7 to 8 DAI and erupted 3 days later exuding columns of urediniospores similar in size to the initially collected isolates. Inoculating another set of detached leaves with a spore suspension (1 × 106 spores per ml) from the first set of detached leaves resulted in typical rust symptoms. The PCR assay, alignment of ITS region 2, morphological characters of the isolates, and pathogenicity tests demonstrate that P. pachyrhizi occurs in Ghana. To our knowledge, this is the first report of P. pachyrhizi in Ghana. References: (1) O. A. Akinsanmi et al. Plant Dis. 85:97, 2001. (2) R. D. Frederick et al. Phytopathology 92:217, 2002. (3) M. Twizeyimana et al. Online publication. http://www.plantmanagementnetwork.org/ infocenter/topic/soybeanrust/2006/posters/41.asp. Plant Management Network, 2006. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/30780460/First_Report_of_Soybean_Rust_Caused_by_Phakopsora_pachyrhizi_in_Ghana_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-91-8-1057B?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -