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Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model.
Int J Mol Sci 2019; 20(4)IJ

Abstract

This study was designed to investigate the potential effects and underlying mechanism of adipose tissue-derived mesenchymal stem cells (MSCs) on allergic inflammation compared to Montelukast as an antileukotriene drug in a rat model of allergic rhinitis (AR). The effect of MSCs was evaluated in albino rats that were randomly divided into four (control, AR, AR + Montelukast, and AR + MSCs) groups. Rats of AR group were sensitized by ovalbumin (OVA) and then challenged with daily nasal drops of OVA diluted in sterile physiological saline (50 μL/nostril, 100 mg/mL, 10% OVA) from day 15 to day 21 of treatment with/without Montelukast (1 h before each challenge) or MSCs I/P injection (1 × 10⁶ MCSs; weekly for three constitutive weeks). Both Montelukast and MSCs treatment started from day 15 of the experiment. At the end of the 5th week, blood samples were collected from all rats for immunological assays, histological, and molecular biology examinations. Both oral Montelukast and intraperitoneal injection of MSCs significantly reduced allergic symptoms and OVA-specific immunoglobulin E (IgE), IgG1, IgG2a and histamine as well as increasing prostaglandin E2 (PGE2). Further analysis revealed that induction of nasal innate cytokines, such as interleukin (IL)-4 and TNF-α; and chemokines, such as CCL11 and vascular cell adhesion molecule-1 (VCAM-1), were suppressed; and transforming growth factor-β (TGF-β) was up-regulated in Montelukast and MSCs-treated groups with superior effect to MSCs, which explained their underlying mechanism. In addition, the adipose tissue-derived MSCs-treated group had more restoring effects on nasal mucosa structure demonstrated by electron microscopical examination.

Authors+Show Affiliations

Department of Histology and Cell Biology, Benha University, Benha, Qalyubia 13518, Egypt. nesrien.salem@fmed.bu.edu.eg. Stem Cell Unit, Benha University, Benha, Qalyubia 13518, Egypt. nesrien.salem@fmed.bu.edu.eg.Department of Otorhinolaryngology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt. yasser.mandour@fmed.bu.edu.eg.Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalyubia 13736, Egypt. ayman.samir@fvtm.bu.edu.eg.Zoology Department, Faculty of Science, Benha University, Benha 13518, Egypt. ebtesam.nafe@fsc.bu.edu.eg.Department of Microbiology, Faculty of Science, Tanta University, Tanta 31527, Egypt. a.shenawy.32016@alexu.edu.eg.Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. Miriam.safwat@kaseralainy.edu.eg.Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. radwa.taha@kaseralainy.edu.eg.Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. dinasabry@kasralainy.edu.eg. Molecular Biology and Stem Cell Unit, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. dinasabry@kasralainy.edu.eg.Department of Clinical Pharmacology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt. safwa.sorour@fmed.bu.edu.eg.Department of Otorhinolaryngology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt. ahmed.alrefaai@fmed.bu.edu.eg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30781605

Citation

Ebrahim, Nesrine, et al. "Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model." International Journal of Molecular Sciences, vol. 20, no. 4, 2019.
Ebrahim N, Mandour YMH, Farid AS, et al. Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model. Int J Mol Sci. 2019;20(4).
Ebrahim, N., Mandour, Y. M. H., Farid, A. S., Nafie, E., Mohamed, A. Z., Safwat, M., ... Refae, A. (2019). Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model. International Journal of Molecular Sciences, 20(4), doi:10.3390/ijms20040873.
Ebrahim N, et al. Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model. Int J Mol Sci. 2019 Feb 18;20(4) PubMed PMID: 30781605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model. AU - Ebrahim,Nesrine, AU - Mandour,Yasser Mohammad Hassan, AU - Farid,Ayman Samir, AU - Nafie,Ebtesam, AU - Mohamed,Amira Zaky, AU - Safwat,Miriam, AU - Taha,Radwa, AU - Sabry,Dina, AU - Sorour,Safwa M, AU - Refae,Ahmed, Y1 - 2019/02/18/ PY - 2019/01/30/received PY - 2019/02/13/revised PY - 2019/02/14/accepted PY - 2019/2/21/entrez PY - 2019/2/20/pubmed PY - 2019/2/20/medline KW - CCL11 KW - Montelukast KW - VCAM-1 KW - allergic rhinitis KW - antileukotriene KW - immunoglobulins KW - mesenchymal stem cells JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 4 N2 - This study was designed to investigate the potential effects and underlying mechanism of adipose tissue-derived mesenchymal stem cells (MSCs) on allergic inflammation compared to Montelukast as an antileukotriene drug in a rat model of allergic rhinitis (AR). The effect of MSCs was evaluated in albino rats that were randomly divided into four (control, AR, AR + Montelukast, and AR + MSCs) groups. Rats of AR group were sensitized by ovalbumin (OVA) and then challenged with daily nasal drops of OVA diluted in sterile physiological saline (50 μL/nostril, 100 mg/mL, 10% OVA) from day 15 to day 21 of treatment with/without Montelukast (1 h before each challenge) or MSCs I/P injection (1 × 10⁶ MCSs; weekly for three constitutive weeks). Both Montelukast and MSCs treatment started from day 15 of the experiment. At the end of the 5th week, blood samples were collected from all rats for immunological assays, histological, and molecular biology examinations. Both oral Montelukast and intraperitoneal injection of MSCs significantly reduced allergic symptoms and OVA-specific immunoglobulin E (IgE), IgG1, IgG2a and histamine as well as increasing prostaglandin E2 (PGE2). Further analysis revealed that induction of nasal innate cytokines, such as interleukin (IL)-4 and TNF-α; and chemokines, such as CCL11 and vascular cell adhesion molecule-1 (VCAM-1), were suppressed; and transforming growth factor-β (TGF-β) was up-regulated in Montelukast and MSCs-treated groups with superior effect to MSCs, which explained their underlying mechanism. In addition, the adipose tissue-derived MSCs-treated group had more restoring effects on nasal mucosa structure demonstrated by electron microscopical examination. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/30781605/Adipose_Tissue_Derived_Mesenchymal_Stem_Cell_Modulates_the_Immune_Response_of_Allergic_Rhinitis_in_a_Rat_Model_ L2 - http://www.mdpi.com/resolver?pii=ijms20040873 DB - PRIME DP - Unbound Medicine ER -